Callus induction of leaf expalnts of Talinum paniculatum

Prianangkai, Karuppiah; Thong, Weng Hing

doi:10.15224/978-1-63248-108-5-02

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Research on callus induction on Talinum paniculatum leaf explants conducted by Karuppiah and Thong (2016) stated that explants grown on MS media added 2.0 mg L -1 2.4-D and 2.5 mg L -1 BAP was the best percentage that is, 100% and the resulting callus is white and friable.…”

Section: Effect Of Pitcher Plant Species and 24-d Concentration On Callus Morphologymentioning

confidence: 99%

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

Novitasari

Isnaini²

2021

Agric

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In vitro propagation of pitcher plants is still limited only using seeds, while the other in vitro methods using leaf explants such as embryogenesis, organogenesis, and callus culture has not been widely reported. The research aims to study the growth response of leaf explants in two species of pitcher plants (Nepenthes gracilis and Nepenthes reinwardtiana), which formed callus in several treatment media combinations. Leaf pieces were taken from a 4-month pitcher plant culture were grown on the treatment media, namely modified media of Murashige & Skoog with a half concentration (½ MS) added 2.4-Dichlorophenoxoxyacetate (2.4-D) with a concentration of 0, 0.5, 1, 1.5, and 2 mg L-1 and kinetin (0.5 mg L-1). The treatment was then stored in a dark environment to induce callus formation. The observation for 12 weeks showed that the time of callus formation in two species of pitcher plant observed was not significantly different. Both species of pitcher plants begin to form callus in the fourth week after treatment. None of the leaf explants were planted on the control medium without hormones formed callus. The best medium for callus induction in N. gracilis is ½ MS medium added with 2 mg L-1 2,4-D and 0.5 mg L-1 kinetin, with callus morphology brownish-white with friable texture. In comparison, the optimum callus media from leaf explant of N. reinwardtiana has not been obtained yet. Thus further research is still needed.

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Section: Effect Of Pitcher Plant Species and 24-d Concentration On Callus Morphologymentioning

confidence: 99%

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

Novitasari

Isnaini²

2021

Agric

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“…Penambahan BA juga diketahui memberikan hasil tertinggi pada pengamatan tinggi tunas. Karuppiah & Hing (2016) menyatakan bahwa inisiasi kalus pada tanaman Talinum paniculatum dengan perlakuan kombinasi ZPT 2,4-D 2 mg/l dan BA 2,5 mg/l menunjukkan hasil terbaik dengan persentase tumbuhnya kalus sebanyak 100%. Perlakuan dengan persentase tumbuhnya kalus terendah diperoleh pada perlakuan ZPT yang auksin dan sitokinin digunakan secara tunggal dengan persentase tumbuh kalus 0%.…”

unclassified

Inisiasi Kalus Daun Talinum triangulare (Jacq.) Willd pada Beberapa Kombinasi Konsentrasi Zat Pengatur Tumbuh 2,4-Dichlorophenoxyatic Acid dan Benzyl Adenine

Anggraeni

Ismaini²,

Surya³

et al. 2022

Agrikultura

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Ginseng Jawa (Talinum triangular) merupakan tanaman yang seluruh bagian tanamannya memiliki khasiat sebagai obat. Tujuan penelitian ini adalah optimalisasi zat pengatur tumbuh untuk inisasi kalus ginseng jawa. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Kebun Raya Cibodas – Badan Riset dan Inovasi Nasional (BRIN) pada bulan Agustus sampai bulan November 2021. Metode penelitian yang digunakan adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan sembilan kombinasi perlakuan 2,4-D (Dichlorophenoxyatic Acid) and Benzyl Adenine (BA) dengan tiga ulangan. Eksplan daun berdiameter 8 mm ditanam pada botol kultur berisi media Murashige and Skoog yang mengandung kombinasi konsentrasi zat pengatur tumbuh 2,4-D (0,5 ppm, 1 ppm, dan 1,5 ppm) dan BA (0,5 ppm, 1 ppm, dan 1,5 ppm). Eksplan yang ditanaman mampu menghasilkan kalus dengan waktu awal muncul kalus antara 13-22 hari setelah inisiasi dan memiliki tekstur remah pada semua perlakuan. Terdapat empat warna kalus yang dihasilkan dari perlakuan ini, yaitu moderate yellow green, grayish yellow green, pale olive dan pale brown. Terdapat kombinasi konsentrasi terbaik diperoleh pada perlakuan D2B3 (1 ppm 2,4-D + 1,5 ppm BA) yang memberikan pertumbuhan optimal pada parameter panjang kalus sebesar 3,17 cm dan parameter berat basah kalus sebesar 2,16 gram serta berat kering kalus sebesar 0,21 gram.

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Callus induction of leaf expalnts of Talinum paniculatum

Cited by 2 publications

References 5 publications

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

PROPAGATION OF PITCHER PLANTS (Nepenthes gracilis KORTH. AND Nepenthes reinwardtiana MIQ.) THROUGH CALLUS INDUCTION

Inisiasi Kalus Daun Talinum triangulare (Jacq.) Willd pada Beberapa Kombinasi Konsentrasi Zat Pengatur Tumbuh 2,4-Dichlorophenoxyatic Acid dan Benzyl Adenine

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