A Defined Culture Medium Suitable for Sensitive Phalaenopsis Species 
 Seedlings

Choong, Chieh Wean; Choong, Siew Mee; Thong, Weng Hing

doi:10.3923/biotech.2013.163.167

Cited by 2 publications

(4 citation statements)

References 8 publications

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“…This was for the sterilization of the seedpods. The seedpods were then flamed briefly until traces of ethanol burnt off, excised with a scalpel and seeds within the seedpods were inoculated onto CCT medium (Choong et al, 2013). Seedlings were maintained on the same medium with a 60-day subculturing regime.…”

Section: Methodsmentioning

confidence: 99%

“…Each treatment had 23 replicates. CCT medium contained (mgL) NH 4 NO 3 (190) NaCl (20), half strength of MS micro-nutrients, myo-inositol (100), niacin (0.5), pyridoxine HCl (0.5), thiamine HCl (0.1), glycine (2), sucrose (25,000) and gellan gum (2,200) at pH 5.2 (Choong et al, 2013). The amount of KNO 3 was reduced from 370 to 350 mg L 1 to achieve NPK ratio of 2.0:0.8:3.0.…”

Section: Methodsmentioning

confidence: 99%

“…Phalaenopsis species seedlings were found to grow well on (Choong et al, 2013) medium which was shown to be non-genotype selective. However, optimum level of MS micronutrient in this medium is unknown; therefore the optimum MS micronutrient level for P. deliciosa seedling yield on CCT medium was investigated.…”

Section: Ojbsmentioning

confidence: 99%

“…Therefore, deficient in Mn supply gave rise to low enzyme activity and subsequently may lead to reduction of protein, nucleic acid and chlorophyll synthesis. ------------------------------------------------------------------------------------------------------------ Choong et al (2013), New Dogashima Medium (NGM)- Tokuhara and Mii (1993); Ernst (1994)…”

Section: Micronutrient Deficiencymentioning

confidence: 99%

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OPTIMUM MICRONUTRIENT LEVEL FOR PHALAENOPSIS DELICIOSA ORCHID SEEDLING IN VITRO GROWTH

Xiong¹,

Choong²

2014

OnLine Journal of Biological Sciences

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There were many researches about the influence of micronutrient concentration toward growth of plants. However, there was no clear statement about the micronutrient level for growth of Phalaenopsis seedlings. Hence it was worth to investigate the optimum level of micronutrient for Phalaenopsis, an endangered orchid species. Using Phalaenopsis deliciosa as the subject, germinated seedlings were grown on defined culture media containing different MS micronutrient level. After 90 days with a subculture at day 45, fresh and dry weights of shoots and roots of the seedlings were measured. It was found that the optimum micronutrient level for P. deliciosa seedling growth was observed between 0.50× and 1.00× of MS micronutrient level. Higher micronutrient level caused roots and seedlings to deterioration except for a minority of seedling variants that grew exceptionally well, suggesting that high micronutrient level was selective for a small number of variants. The study demonstrated the importance of appropriate micronutrient level for supporting growth and development for wide range of genotypes in P. deliciosa. This micronutrient level may as well be optimum for other species under the genus Phalaenopsis and should be considered for maintaining genotype diversity in vitro.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Ojbsmentioning

confidence: 99%

Section: Micronutrient Deficiencymentioning

confidence: 99%

See 2 more Smart Citations

OPTIMUM MICRONUTRIENT LEVEL FOR PHALAENOPSIS DELICIOSA ORCHID SEEDLING IN VITRO GROWTH

Xiong¹,

Choong²

2014

OnLine Journal of Biological Sciences

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Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth

Choong¹,

Lim²,

Xiong³

et al. 2016

OnLine Journal of Biological Sciences

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Abstract:In a continuous effort to conserve Phalaenopsis species, the optimization of a previously designed defined medium was carried out to understand the effect of in vitro nitrogen nutrition on Phalaenopsis deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient level instead of solely Nitrogen (N) was adjusted. Seedlings were obtained from in vitro germination and randomly inoculated on media with various macronutrient levels (0.3×, 0.5×, 1×, 2× and 3×). Maximum shoot yield was observed at 2× macronutrient level. However, the best macronutrient level, which was 1× macronutrient level, resulted in the maximum root and seedling yield. This macronutrient level was applied on the subsequent experiment where seedlings were inoculated on media with various nitratenitrogen (NO 3 -N) to ammonium-nitrogen (NH 4 -N) ratios. Seedling yield was the highest at NO 3 -N/NH 4 -N ratio of 2.0 while Root to Shoot ratio (R/S) was the highest at NO 3 -N/NH 4 -N ratio of 5.0. NO 3 -N/NH 4 -N ratio significantly affected Water Content (WC) of seedlings and the response to NO 3 -N/NH 4 -N ratio was similar to R/S, correlating better root development to higher WC. By altering NO 3 -N/NH 4 -N ratio, plant quality in terms of yield and organ development could be encouraged.

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A Defined Culture Medium Suitable for Sensitive Phalaenopsis Species Seedlings

Cited by 2 publications

References 8 publications

OPTIMUM MICRONUTRIENT LEVEL FOR <i>PHALAENOPSIS DELICIOSA</i> ORCHID SEEDLING <i>IN VITRO</i> GROWTH

OPTIMUM MICRONUTRIENT LEVEL FOR <i>PHALAENOPSIS DELICIOSA</i> ORCHID SEEDLING <i>IN VITRO</i> GROWTH

Effect of <i>In Vitro</i> Nitrogen Nutrition to <i>Phalaenopsis deliciosa</i> Seedling Growth

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