A bacterial strain capable of rapidly degrading di-2-ethylhexyl phthalate (DEHP) was isolated from soil and identified as Bacillus subtilis. The organism also utilized di-butyl phthalate, di-ethyl phthalate, di-pentyl phthalate, di-propyl phthalate, and phthalic acid as sole carbon sources; and their biodegradation ratio was over 99%, when the incubation was performed for 5 days at 30 degrees C. The microorganism degraded di-2-ethylhexyl phthalate and di-butyl phthalate through the intermediate formation of mono-2-ethylhexyl phthalate and mono-butyl phthalate, which were then metabolized to phthalic acid and further by a protocatechuate pathway, as evidenced by oxygen uptake studies and GC-MS analysis. The decontamination of soil polluted with di-2-ethylhexyl phthalate by B. subtilis was investigated. Experimental results showed that the strain could degrade about 80% of 5 mM DEHP simply by adding 8% culture medium to soil, indicating that the degradation can occur even when other organisms are present.
Fiber optic taper has been used more and more widely as a relay optical component in the integrated taper assembly image intensified sensors for military and medical imaging application. In this paper, the transmission characteristic of energy in the taper is analyzed, and following the generalized definition of the modulation transfer function for sampled imaging system, a spatial averaged impulse response and a corresponding MTF component that are inherent in the sampling process of taper are deduced, and the mathematical model for evaluating the modulation transfer function of fiber optic taper is built. Finally, the dynamic and static modulation transfer function curves simulated by computer have been exhibited.
A bacterial strain, designated LYBFD3-16A2(T), was isolated from tribenuron methyl contaminated wheat soil. Cells were observed to be Gram-negative short rods with a single flagellum. The strain was found to utilize methanol, glucose, maltose and mannitol as carbon and energy sources, and utilized glutamate, leucine, phenylalanine as organic nitrogen sources. Strain LYBFD3-16A2(T) was found to be aerobic, to form urease, produce hydrogen sulfide and reduce nitrate to nitrite. The indole test in tryptone broth was observed to be positive. The major cellular fatty acids were identified as C18:1ω7c (81.3 %), 11-methylC18:1ω7c (7.9 %), C18:0 (3.0 %) and C16:0 (3.0 %). The major phospholipids were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidyglycerol and diphosphatidylglycerol. The main ubiquinone was identified as Q-10. The DNA G+C content was determined to be between 70.2 and 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated the affiliation of strain LYBFD3-16A2 to members of the genus Methylopila. The DNA-DNA hybridization values of the novel strain with the type strains of the most closely related species Methylopila musalis MUSA(T) and Methylopila jiangsuensis JZL-4(T) were 35.4 % and 31.4 % respectively. The genotypic and phenotypic characterization, along with chemotaxonomic properties of strain LYBFD3-16A2(T), showed that the strain represents a novel species of the genus Methylopila for which the name Methylopila henanense sp. nov. is proposed. The type strain is LYBFD3-16A2(T) (=CGMCC1.10703(T) = LMG 25959(T)).
As a relay optical component, fiber optic taper has become more important and more widely used in the integrated taper assembly image intensified sensors for military and medical imaging application. However, the method for evaluating the quality of output images from the fiber optic taper is few. In this article, the Modulation Transfer Function (MTF) is introduced and measured to evaluate the image quality of fiber optic taper. Because fiber optic taper is of a mosaic array architecture, there are some new problems need to be resolved in defining MTF of it. And there also should be some special requirements in MTF measurement. Two methods including edge scanning and point imaging analyzing are introduced in measuring MTF of fiber optic taper, in which the incident light, scanning step and scanning range are specially limited. Experiments show that the results of the measurements consist with the theoretical analysis simulated by computer. And the spatial averaged MTF of fiber optic taper has been gotten by two means. It is concluded that the MTF value measured by point imaging is always some higher than measured by edge scanning, because the point imaging is more weakly effected by the fiber arrangement in taper.
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