Methylopila henanense sp. nov., a novel methylotrophic bacterium isolated from tribenuron methyl-contaminated wheat soil

Abstract

A bacterial strain, designated LYBFD3-16A2(T), was isolated from tribenuron methyl contaminated wheat soil. Cells were observed to be Gram-negative short rods with a single flagellum. The strain was found to utilize methanol, glucose, maltose and mannitol as carbon and energy sources, and utilized glutamate, leucine, phenylalanine as organic nitrogen sources. Strain LYBFD3-16A2(T) was found to be aerobic, to form urease, produce hydrogen sulfide and reduce nitrate to nitrite. The indole test in tryptone broth was observed to be positive. The major cellular fatty acids were identified as C18:1ω7c (81.3 %), 11-methylC18:1ω7c (7.9 %), C18:0 (3.0 %) and C16:0 (3.0 %). The major phospholipids were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidyglycerol and diphosphatidylglycerol. The main ubiquinone was identified as Q-10. The DNA G+C content was determined to be between 70.2 and 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated the affiliation of strain LYBFD3-16A2 to members of the genus Methylopila. The DNA-DNA hybridization values of the novel strain with the type strains of the most closely related species Methylopila musalis MUSA(T) and Methylopila jiangsuensis JZL-4(T) were 35.4 % and 31.4 % respectively. The genotypic and phenotypic characterization, along with chemotaxonomic properties of strain LYBFD3-16A2(T), showed that the strain represents a novel species of the genus Methylopila for which the name Methylopila henanense sp. nov. is proposed. The type strain is LYBFD3-16A2(T) (=CGMCC1.10703(T) = LMG 25959(T)).

Microbial contamination in methanol biofilters inoculated with a pure strain of Pichia pastoris: A potential limitation for waste revalorization

“…31,32 Other found microorganisms were Stenotrophomonas sp., Achromobacter sp., Actinomyces sp., Alcaligenes faecalis, Burkholderia cepacia, Burkholderia sp., Enterobacter sp., Lactococcus lactis, Mesorhizobium sp., Methylopila henanense, Pseudomonas sp., Serratia sp., and Stenotrophomonas sp., which might be involved in the consumption of formaldehyde and formate ( Table 2). 27,[30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] Altogether, the cloning and sequencing results clearly suggested that most of the bacteria, most of the microorganisms, that colonized the biofilters were potentially associated to the consumption of formaldehyde. However, the presence of Methylopila henanense and Pseudomonas sp.…”

“…Moreover, metabolic reconstruction from genomic analysis has indicated that K. oxytoca can also degrade and use formaldehyde as carbon source or even use methanol if H 2 O 2 is present . Other found microorganisms were Stenotrophomonas sp., Achromobacter sp., Actinomyces sp., Alcaligenes faecalis , Burkholderia cepacia , Burkholderia sp., Enterobacter sp., Lactococcus lactis , Mesorhizobium sp., Methylopila henanense , Pseudomonas sp., Serratia sp., and Stenotrophomonas sp., which might be involved in the consumption of formaldehyde and formate (Table ) . Altogether, the cloning and sequencing results clearly suggested that most of the bacteria, most of the microorganisms, that colonized the biofilters were potentially associated to the consumption of formaldehyde.…”

“…However, the presence of Methylopila henanense and Pseudomonas sp. was of special interest because they were the only two species (apart of P. pastoris ) capable to oxidize methanol . These organisms were only found in BF1 at the end of Stage 1 and in BF3 at the end of Stage 3.…”

Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales)

“…In the API ZYM, both strains were positive for alkaline phosphatase, esterase, esterase lipase, acid phosphatase, and naphthol-AS-BIphosphohydrolase, and were negative for all tests except for the enzymatic activities, where differences were observed as shown below. Data from two other strains of the Hyphomicrobiales (Rhizobiales) were used for comparison purposes; 3, Pinisolibacter [36]; 4, Methylopila [37].…”