Feline alkaline phosphatase and alkaline phosphatase isoenzymes have been studied in tissue and serum. Alkaline phosphatase from various organs was quantitated and then subjected to cellulose acetate electrophoresis. The effects of bile duct ligation, prednisolone treatment and phenobarbital treatment on serum alkaline phosphatase was measured. The diagnostic importance of feline serum alkaline phosphatase levels is discussed in light of the results of this and other studies.

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The primary purpose of this study was to establish plasma biochemistry parameters for healthy recently wild-caught purple mouth moray eels (Gymnothorax vicinus) to provide a baseline of data for improved medical care in an aquarium or zoologic setting and for wild health assessments. Thirty-one clinically healthy purple mouth moray eels of unknown age and sex were caught from the wild, and were anesthetized 50 days following capture for blood collection from the ventral coccygeal vein. The median plasma biochemistry values were as follows: hematocrit = 21%, creatinine kinase = 2,100 U/L, lactate dehydrogenase = 97 U/L, aspartate aminotransferase = 88 U/L, alanine aminotransferase = 51 U/L, alkaline phosphatase 3,939 U/L, gamma-glutamyl transpeptidase = 1 U/L, amylase = 40 U/L, blood urea nitrogen = < 11 mg/dl, glucose = 21 mg/dl, calcium = 12.5 mg/dl, triglyceride = 206 mg/dl, creatinine = 0.1 mg/dl, cholesterol = 334 mg/dl, total bilirubin = < 0.1 mg/dl, phosphorus = 6.5 mg/dl, total protein = 4.2 g/dl, albumin = 1.5 g/dl, globulin = 2.7 g/dl, albumin/ globulin ratio = 0.6, sodium = 185 mmol/L, potassium = 3.7 mmol/L, and chloride = 175 mmol/L. Alkaline phosphatase isoenzyme results indicate that the majority of the plasma alkaline phosphatase is the liver isoenzyme. The data acquired in this study also provide baseline values for cholesterol and triglycerides in recently wild-caught moray eels to aid in monitoring elevations to these values in an aquarium setting over time so adjustments to the dietary regime may be utilized to prevent or improve conditions such as lipid keratopathy.

A 2-year-old Holstein heifer with a swollen brisket, jugular vein distention, muffled heart sounds, tachycardia, and free gas bloat was examined. Thymic lymphosarcoma was suspected based on a negative agar gel immunodiffusion test for bovine leukemia virus, presence of atypical lymphocytes in pleural fluid, and detection of a mass in the thoracic inlet. Right-sided cardiac catheterization was performed, and markedly increased jugular venous pressures (41 m m Hg) hymic lymphosarcoma, juvenile multicentric lympho-T sarcoma, and cutaneous lymphosarcoma are 3 sporadic forms of bovine lymphosarcoma that are not associated with bovine leukemia virus (BLV) infection.' Thymic lymphosarcoma produces clinical signs related to an anterior mediastinal mass, such as cervical and submandibular edema, bilateral jugular vein distention, and varying degrees of ruminal tympany, as well as dyspnea, anorexia, and weight loss.',' The clinical signs of thymic lymphosarcoma can therefore be difficult to differentiate from right-sided heart failure (RSHF). In this report, we describe the clinical and pathological findings in a heifer with thymic lymphosarcoma. Thoracocentesis, ultrasonography, electrocardiography, cardiac catheterization, and immunohistochemical staining pennitted detailed characterization and classification of this case. Case DescriptionA 2-year-old Holstein heifer was examined because of a 6-day history of a swollen brisket, anorexia, and free gas bloat. The heifer had been previously treated for bloat by oral-ruminal passage of a stomach tube and intraruminal administration of liquid fat and magnesium hydroxide. A magnet (PO), procaine penicillin G (IM), and ceftiofur sodium (IM) (doses unknown) had also been administered for treatment of suspected traumatic reticulopericarditis.The heifer appeared depressed and had bilateral abdominal distention. Rectal temperature and respiratory rate were within normal limits, while the pulse rate was increased (1 05 beatshinute). The heart and lung sounds were difficult to auscultate over the entire left side of the thorax, but could be heard normally over the right side of the thorax. Pitting edema extended from the thoracic inlet to the ramus of the mandible, and both jugular veins were greatly distended. Edema and venous distention were not evident caudal to the brisket. Palpation of the brisket revealed a 10-cm diameter, firm mass that extended deep into the thoracic inlet. The mass was fixed in place and surrounded by a markedly edematous subcutis, and the cervical skin was cold to the touch. Palpation per rectum revealed ruminal gas distention and a gravid uterus of approximately 7 months gestation. The feces were dark green and contained frank blood and mucus. A 3-cm diameter stomach tube was easily passed into the mmen and a large volume of gas released with subsequent decrease in abdominal distention.The heifer had a normal blood leukocyte count (9,000 cells/pL; normal, 4,000 to 12,000 cells/pL), normal lymphocyte count (3,150 cells/pL; normal, 2,500 to 7,500 cells/pL),...

Swine were fed a diet containing hexachlorobenzene (HCB) in order to generate contaminated pork which was subsequently cooked and fed to female cats. Feeding the fresh weight equivalent of 50 g cooked pork/day/cat (1 < mg HCB/kg/day) caused marginal weight loss. Oral dosing with 10 mg/kg/day purified HCB resulted in significant weight loss, mild anemia, neutropenia and hepatomegaly. Using the residue of HCB accumulating in the cat adipose tissue as the criterion, the low doses in the pork were absorbed and retained about five times more effectively than the larger oral bolus.

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