Characterization and localization of alkaline phosphatase in canine seminal plasma and gonadal tissues

Kutzler, Michelle Anne; Solter, Philip F.; Hoffman, W. E.; Volkmann, D.H.

doi:10.1016/s0093-691x(02)01366-3

Cited by 27 publications

(17 citation statements)

References 17 publications

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“…The average AP level in elephants was 500.78±615.9 (U/L) and was substantially lower compared to values reported for other species (e.g. boar [35], canine [36], stallion [33], [37], rhino [31], and beluga whale [38]). Although our results failed to find a significant relationship between AP levels and sperm quality (i.e.…”

Section: Discussioncontrasting

confidence: 58%

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

et al. 2013

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Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and physiology, including sperm motility, the objectives of this study were to characterize the chemistry and protein profiles of Asian elephant seminal plasma and to determine the relationships between seminal plasma components and semen quality. Ejaculates exhibiting good sperm motility (≥65%) expressed higher percentages of spermatozoa with normal morphology (80.3±13.0 vs. 44.9±30.8%) and positive Spermac staining (51.9±14.5 vs. 7.5±14.4%), in addition to higher total volume (135.1±89.6 vs. 88.8±73.1 ml) and lower sperm concentration (473.0±511.2 vs. 1313.8±764.7×106 cells ml−1) compared to ejaculates exhibiting poor sperm motility (≤10%; P<0.05). Comparison of seminal plasma from ejaculates with good versus poor sperm motility revealed significant differences in concentrations of creatine phosphokinase, alanine aminotransferase, phosphorus, sodium, chloride, magnesium, and glucose. These observations suggest seminal plasma influences semen quality in elephants. One- and two-dimensional (2D) gel electrophoresis revealed largely similar compositional profiles of seminal plasma proteins between good and poor motility ejaculates. However, a protein of ∼80 kDa was abundant in 85% of ejaculates with good motility, and was absent in 90% of poor motility ejaculates (P<0.05). We used mass spectrometry to identify this protein as lactotransferrin, and immunoblot analysis to confirm this identification. Together, these findings lay a functional foundation for understanding the contributions of seminal plasma in the regulation of Asian elephant sperm motility, and for improving semen collection and storage in this endangered species.

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Section: Discussioncontrasting

confidence: 58%

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

et al. 2013

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“…It may be that such a high decrease in ALP activity was due to temperature denaturation of this protein. The high coefficients of correlation (between sperm concentration and ALP activity) support earlier opinions that in the case of canid semen, the highest alkaline phos-phatase activity is characteristic of the secretion from epididymides, which serve important functions in the sperm maturation process (Kutzler et al 2003). In turn, acid phosphatase, which is found in the sperm cell membrane, plays an important role in the conversion of proacrosin into acrosin (Fraser et al 2006).…”

Section: Discussionsupporting

confidence: 76%

Use of biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)

Stasiak¹,

Demianowicz²,

Kowalski³

et al. 2014

Polish Journal of Veterinary Sciences

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The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according to the stage of cryopreservation (fresh -measurement A; equilibrated -measurement B; frozen/thawed -measurement C). Furthermore, the seminal plasma and supernatants were analysed after equilibration and freeze/thawing for the activity of the enzymes alkaline phosphatase (ALP), acid phosphatase (AcP), lactate dehydrogenase (LDH) and aspartate aminotransferase (AspAT), and for the activity of acrosin inhibitors (AP). The mean concentration of sperm was 625.1 million/cm 3 , and ejaculate volume averaged 1.6 cm 3 . Seminal plasma was characterized by the highest activity of alkaline phosphatase (3.43×10 3 U/l) and lowest activity of acrosin inhibitors (4.55×10 3 U/l).After equilibration, the supernatants showed the highest activity of acid phosphatase (94.9 U/l) and after freeze-thawing, they showed a high activity of lactate dehydrogenase (535.8 U/l) and aspartate aminotransferase (577.1 U/l), which indicates that these proteins had leaked from spermatozoa into the extracellular medium during the biotechnique of semen cryopreservation. In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity.

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“…These isoenzymes have different roles based on glucoprotein type in their structures (23). In male reproduction tract, ALP is secreted in prostate and testes (24) Seminal fluid quality could be determined by isoenzymes of ALP (25). Effectiveness role of alkaline phosphatase is seen in Leyding cell, transitional and Fibroblast in seminiferous tubules (26).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Testosterone and Alkaline Phosphatase Activity Changes in Epidydimis of Toxoplasma gondii Infected Rats

Afshari¹,

Imani²,

Asl³

et al. 2013

IJWHR

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Characterization and localization of alkaline phosphatase in canine seminal plasma and gonadal tissues

Cited by 27 publications

References 17 publications

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

Use of biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)

Evaluation of Testosterone and Alkaline Phosphatase Activity Changes in Epidydimis of Toxoplasma gondii Infected Rats

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