The paper presents the results of investigations of genotoxicity and morphological characteristics of nanoscale gold particles prepared by the electric spark dispersion method in a water medium. To investigate the properties we used comet assay, transmission electron microscopy, thermal desorption of nitrogen (BET). It was revealed, gold particles did not cause DNA damage at low concentrations (0.01 - 0.1 mg/ml) and detectable level of genotoxicity of gold nanoparticles is observed at concentrations of 0.3 mg/ml and above.

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Objective: Important trend in the global agribusiness is restrictions of antibiotics content in meat and poultry products. Widespread of antibioticresistant microorganisms leads to huge losses of the effectiveness of the drugs. The aim of the present work was an investigation of the biological activity of antibacterial complex-silver methenamine poultry farming. Methods:The study of biochemical and hematological indices of blood we performed one day after single and course of oral drug administration on broilers Ross 308. Evaluation of biological action in industrial poultry farming was carried out on a group of 30 thousand broilers after a course of oral administration.Results: revealed the silver complex (in form of water solution) has no toxic effects on blood parameters. It was shown the positive influence on survival and productivity of broilers. Conclusion:Methenamine silver has certain potential as prospect drug and food additive.

Background: Multiple drug resistance of tumor cells is a serious challenge in modern medicine. Neoplastic diseases are often complicated by persistent bacterial infections, which impairs efficacy and the outcome of anticancer therapy. The development of new bifunctional drugs for the treatment of tumor growth and bacterial complications is an urgent task. The objective of this study was to evaluate the complex antiblastic and antibacterial properties of interferon-platinum conjugate in vitro. Material and Methods: The coordination complex of dichloro-N,N,N,N-tetrakis-(2-aminoethyl)-1,6-hexamethylenediaminobisplatin dichloride was used for production of conjugate with human α-interferon. The binding of a metal complex with protein was established by means of radiochemical neutron activation analysis. As test objects, we used lympholeukemic Jurkat cells line, human lymphocytes in reaction of lymphocyte blastic transformation and different strains of microorganisms, including Staphylococcus aureus and Pseudomonas aeruginosa. Results: Binuclear platinum human α-interferon conjugate has a significant dose-dependent antibacterial and antiproliferative suppressive effect on target cells and bacteria. Conclusion: The tested preparation can be of a certain interest in drug designing for rational antiblastic therapy, especially in the case of infectious complications. This conjugate can be a convenient platform for creating drugs for the complex therapy of oncological diseases with bacterial complications.

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Objective: Developing of novel pharmaceutical agents is an actual topic for chemotherapy. Heavy metals possess significant cytotoxic properties and some of them are widely introduced in medical practice, e. g. cisplatin.Methods: Silver salt of methenamine was suggested as prospective substance and tested was tested on black-motley cattle with bovine leukemia to reveal influence on haematological parameters. The preparation was applied in form of 0.5% solution in different doses and was administered as a single intravenous injection. Blood parameters were determined by triple haematological monitoring. Investigation of cytostatic activity was conducted on the leukemic cells of HUT-102 line in vitro in the concentration range of 0.03-3.0 mg/ml. Results:The results showed normalization of blood parameters in a dose-dependent manner after intravenous injection of methenamine silver salt. It was revealed a pronounced inhibitory effect of the silver-based drug on the leukemic cells both in vitro and in vivo. Control blood test one month after experimental therapy confirmed the stability of the achieved results. Conclusion:The tested drug showed a moderate cytostatic effect. Methenamine silver nitrate can be considered as prospective pharmacological agent. Bovine leukaemia was shown as a convenient model for the development of new treatment of blood diseases. Continuation of the work is of interest for both veterinary and medical pharmacotherapy. Designing of novel cytostatic agents for leucosis treatment is a challenging task for modern pharmacology and medicine. Especially acute problem is a resistant form of decease [1][2]. A lot of modern approaches are used now for development delivery systems and screening of novel agents, e. g. virtual ligand screening [3][4]. Due to drug-resistivity, recently interest revived in the metal preparations of a new generation with cytostatic, antiviral and antibacterial activities [5][6][7][8]. Malignant and infectious diseases is a huge problem in veterinary practice as well, e. g. bovine leucosis causing enormous economic losses, and required new eradication approaches [9][10]. Currently, there are well-developed methods of laboratory diagnosis of bovine leukemia [11], but methods of treatment are not developed at all. Specific prophylaxis is only developed, but not introduced [12]. Bovine leukemia virus (BLV) is a retrovirus closely related to the human T-lymphotropic virus type 1 HTLV-I. Bovine leukemia virus (BLV) is a valuable model system for investigation of human lymphotropic viruses [13]. Here we considered viral bovine leukemia as a very convenient model for testing of promising antiviral and cytostatic agents for the medical practice, where leukemia is still a major problem [14]. The aim of this work is to study the cytostatic activity of stabilized solution of methenamine silver nitrate under in vitro and in vivo conditions. Previously, it was shown a wide range of anti-infective activity of methenamine silver nitrate [15].Investigation of antiblastic activity o...