The increase in bacterial resistance to antibiotics is a global problem for public health. In our previous works, it was shown that the application of AgNPs in cow mastitis treatment increased S. aureus and S. dysgalactiae susceptibility to 31 antibiotics due to a decrease in the bacterial efflux effect. The aim of the present work was to shed light on whether the change in adhesive and anti-lysozyme activities caused by AgNPs also contribute to the restoration of bacterial susceptibility to antibiotics. In vivo sampling was performed before and after cow mastitis treatments with antibiotics or AgNPs. The isolates were identified, and the adhesive and anti-lysozyme activities were assessed. These data were compared with the results obtained for in vitro pre-treatment of reference bacteria with AgNPs or antibiotics. The present study revealed that bacterial treatments in vitro and in vivo with AgNPs: (1) decrease the bacterial ability to adhere to cells to start an infection and (2) decrease bacterial anti-lysozyme activity, thereby enhancing the activity of lysozyme, a natural “antibiotic” present in living organisms. The obtained data contribute to the perspective of the future application of AgNPs for recovering the activity of antibiotics rapidly disappearing from the market.
For surface-enhanced Raman spectroscopy (SERS) sensing
of small
molecules in the presence of living cells, biofouling and blocking
of plasmonic centers are key challenges. Here, we have developed a
mesoporous Au (AuM) film coated with a Ag nanoparticles (NPs) as a
plasmonic sensor (AuM@Ag) to analyze aromatic thiols, which is an
example of a small molecule, in the presence of a living cell strain
(e.g., MDA-MB-231) as a model living system. The resulting AuM@Ag
provides 0.1 nM sensitivity and high reproducibility for thiols sensing.
Simultaneously, the AuM@Ag film filters large biomolecules, preventing
Raman signals from overlapping produced by large biomolecules. After
analysis, the AuM@Ag film undergoes recycling by the full dissolution
of the Ag-thiol layer and removal of thiols from AuM. Furthermore,
fresh AgNPs are formed for further SERS analysis, which circumvents
the Ag oxidation issue. The ease of the AgNPs deposition allows up
to 12 cycles of on-demand recycling and sensing even after utilization
as a sensor in multicomponent media without enhancement and sensitivity
loss. The reported mesoporous film with surface filtering ability
and prominent recycling procedure promises to offer a new strategy
for the detection of various small molecules in the presence of living
cells.
Modified nanostructured sorbents are widely used in water treatment processes. In this work, aluminum and iron particles prepared by electro-spark dispersion have been applied as modifiers. Zeolite samples from "Holinskoe" mineral deposit (Russia, Republic of Buryatia), with a size smaller than 0.1 mm, have been modified with aluminum and iron particles, using a sol-gel process. The properties of the modified materials have been determined by means of sorption test, when removing ions Pb{2+}, Fe{3+} and Cd{2+} from the model solutions in static conditions. Using the method of thermal desorption of nitrogen (BET) it has been shown that increasing the iron content in the samples of nanostructured modified sorbents does not affect the increase in specific surface area and pore volume of the samples. Ions concentrations have been analyzed by stripping voltammetry and photocolorimetry. As a result, modified sorbents revealed a high efficiency sorption of heavy metals
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