Agrimonia pilosa Ldb., a herbaceous plant, has been used in Chinese medicines as anti-haemorrhagic, anthelmintic and anti-inflammatory agent. In this study, the complete chloroplast (cp) genome sequence of A. pilosa was first reported. The cp genome is 155,188 bp in length, with two inverted repeat (IR) regions of 25,965 bp, the large single copy (LSC) region of 84,521 bp and the small single copy (SSC) region of 18,737 bp. A total of 129 genes were predicted, including 84 protein-coding genes, 37 tRNA genes and 8 rRNA genes. The phylogenetic analysis suggested that A. pilosa is more closely related to Sanguisorba filiformis with strong bootstrap values belonging to the subfamily Rosoideae.
Picosecond lasers with high average power and high beam quality have been widely used for precision processing and space exploration. In this study, we report a high-power picosecond green laser using a multistage Yb-doped rod-shaped photonic crystal fiber as an amplifier combined with a beam combination. The single amplification module achieves a 1,030 nm laser output of 146.8 W, and the maximum second harmonic generation (SHG) power is 92 W with a frequency conversion efficiency of 63.5%. The combined beam of the two SHGs resulted in a final output of 178 W with a repetition frequency of 24.07 MHz, pulse width of 50.1 ps, and beam quality factor of M2 = 1.16. Furthermore, an adaptive filter control method of a two-axis fast-steering mirror was applied to suppress the beam jitter to up to 45 Hz.
Tartary buckwheat ( Fagopyrum tataricum) is rich in flavonoids,
which not only play an important role in plant-environment interaction,
but are also beneficial to human health. Rutin is a therapeutic flavonol
which is massively accumulated in Tartary buckwheat. It has been
demonstrated that transcription factors (TFs) control rutin
biosynthesis. However, the transcriptional regulatory network of rutin
is not fully clear. In this study, through transcriptome and target
metabolomics profiling analysis together with homolog prediction, we
identified and validated the role of FtMYB102 and FtbHLH4 TFs at the
different developmental stages of Tartary buckwheat. The elevated
accumulation of rutin in the sprout appears to be closely associated
with the expression of FtMYB102 and FtHLH4. Yeast two-hybrid, transient
luciferase activity and co-immunoprecipitation analysis demonstrated
that FtMYB102 and FtbHLH4 can interact and form a transcriptional
complex. Moreover, yeast one-hybrid showed that both FtMYB102 and
FtbHLH4 directly bind to the promoter of chalcone isomerase (
CHI), and they can coordinately induce CHI expression as
shown by transient luciferase activity assay. Finally, we transferred
the FtMYB102 and FtbHLH4 into the hairy roots of Tartary buckwheat and
found that they both can promote the accumulation of rutin. Our results
indicate that FtMYB102 and FtbHLH4 can form a transcriptional complex by
inducing CHI expression to coordinately promote the accumulation
of rutin.
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