A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum

Mi, Yaolei; Liu, Yu; Qian, Guangtao; Vanhaelewyn, Lucas; Meng, Xiangying; Liu, Tingxia; Yang, Wei; Shi, Yuhua; Ma, Pengda; Tul-Wahab, Atia; Viczián, András; Chen, Shilin; Sun, Wei; Zhang, Dong

doi:10.1016/j.plaphy.2022.12.016

Cited by 5 publications

(2 citation statements)

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“…The number of MYB -related transcription factors was the highest in the rhizomes of P. cyrtonema , accounting for 5.24% of the total TFs. In addition, MYB family members have been found to regulate flavonoid biosynthesis pathways in most species, including Arabidopsis thaliana [ 40 ], Fagopyrum tataricum [ 29 ], Petunia hybrida [ 35 ], Citrus [ 32 ], Fragum [ 41 ], Prunus avium L. [ 25 ], and other species. Therefore, we speculate that MYB transcription factors are significant regulators of the flavonoid biosynthesis pathways of P. cyrtonema , constituting a significant role for promoting the development of the rhizomes of P. cyrtonema , improving resistance and secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Transcription factors (TFs) are key agents that regulate the expression of structural genes at the transcriptional level. Studies have shown that MYB , bHLH , WD40 , NAC , and AP2/ERF are involved in flavonoid biosynthesis [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. The MYB family of transcription factors is one of the largest families of TFs.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis Reveals the Flavonoid Biosynthesis Pathway Involved in Rhizome Development in Polygonatum cyrtonema Hua

Wan,

Ban,

Yang

et al. 2024

Plants

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Polygonatum cyrtonema Hua (P. cyrtonema) rhizomes are rich in flavonoids and other secondary metabolites, exhibiting remarkable antioxidant, anti-tumor, and immunomodulatory effects. Polygonatum flavonoid-biosynthesis-related genes have been characterized already. However, a comprehensive overview of Polygonatum flavonoid biosynthesis pathways is still absent. To articulate the accumulation of the flavonoid biosynthesis pathways, we examined transcriptome changes using Illumina HiSeq from five different tissues and the RNA-seq of 15 samples had over 105 Gb of a clean base, generating a total of 277,955 unigenes. The cDNA libraries of the fruits (F), leaves (L), roots (R), stems (S), and rhizomes (T) of three-year-old P. cyrtonema plants generated 57,591, 53,578, 60,321, 51,530, and 54,935 unigenes. Comparative transcriptome analysis revealed that 379 differentially expressed genes (DEGs) were in the group of F _vs_ T, L _vs_ T, R _vs_ T, and S _vs_ T, and the transcripts of flavonoid-biosynthesis-related DEGs were principally enriched in rhizomes. In addition, combined with WGCNA and the FPKM of five tissues’ transcription, nine differentially expressed transcription factor families (MYB, WRKY, AP2/ERF, etc.) were characterized in the red module, the red module positively correlated with rhizome flavonoid accumulation. Quantitative real-time PCR (qRT-PCR) further indicated that BZIP1, C3H31, ERF114, and DREB21 are differentially expressed in rhizomes, accompanied in rhizome development in P. cyrtonema. Therefore, this study provides a foundation for further research into uncovering the accumulation of flavonoid biosynthesis in the rhizomes of P. cyrtonema.

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