Complete genome and phylogenetic analysis of Agrimonia pilosa ldb

Abstract

Agrimonia pilosa Ldb., a herbaceous plant, has been used in Chinese medicines as anti-haemorrhagic, anthelmintic and anti-inflammatory agent. In this study, the complete chloroplast (cp) genome sequence of A. pilosa was first reported. The cp genome is 155,188 bp in length, with two inverted repeat (IR) regions of 25,965 bp, the large single copy (LSC) region of 84,521 bp and the small single copy (SSC) region of 18,737 bp. A total of 129 genes were predicted, including 84 protein-coding genes, 37 tRNA genes and 8 rRNA genes. The phylogenetic analysis suggested that A. pilosa is more closely related to Sanguisorba filiformis with strong bootstrap values belonging to the subfamily Rosoideae.

The first complete chloroplast genome sequence of Paris polyphylla var. emeiensis, a rare and endangered species

“…The total DNA was extracted using the DNeasy plant mini kit (QIAGEN), and sequenced by Illumina NovaSeq system (Illumina, San Diego, CA, USA). About 7.30 Gb of raw data (51, 901, 266 reads) were assembled by NOVOPlasty (Park et al 2019;Liu et al 2020), and the assembled cp genome was annotated by GeSeq with default sets (Tillich et al 2017;Qian et al 2020). The annotated cp genome was submitted to the GenBank with the accession number of MT381945.…”

“… 2019 ; Liu et al. 2020 ), and the assembled cp genome was annotated by GeSeq with default sets (Tillich et al. 2017 ; Qian et al.…”

The complete chloroplast genome of Agrimonia pilosa Ledeb. isolated in Korea (Rosaceae): investigation of intraspecific variations on its chloroplast genomes

“… 2017 ) and its complete chloroplast genome was also published (Liu et al. 2020 ) but the sequence is not available as of May 2020.…”

Characterization of the complete chloroplast genome of Salvia tiliifolia Vahl (Lamiaceae)

“…Next-generation sequencing was carried out by an Illumina NovaSeq system (San Diego, CA). About 5.6 Gb of raw data (40,013,252 reads) was assembled by NOVOPlasty (Park et al 2019;Liu et al 2020), and the assembled cp genome was annotated by GeSeq with default sets (Tillich et al 2017;Yang et al 2020). The annotated cp genome was submitted to the GenBank with the accession number of MT381946.…”