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Protocorm like bodies (PLBs) derived from callus of Phalaenopsis utilized sucrose , maltose and sorbitol for their growth in vitro. These carbon sources affected differently and could control PLB growth . On sucrose supplemented medium a few PLBs produced plantlets and most others regenerated yellowish or greenish callus like body (CLB). Almost 80% of unrooted and 58% of rooted plantlets developed yellowish green CLB at the base of plantlets. On maltose supplemented medium, PLBs regenerated PLBs and a few plantlets. In subsequent culture, about 44% of unrooted and 24% of rooted plantlets initiated green PLBs at the base of cultured plantlets. On sorbitol supplemented medium, most of the PLBs developed plantlets and a few additional PLBs. Among the carbon sources tested, sorbitol supported plantlet development the best in vitro and proved to be the most suitable carbon source for plantlet initiation and development from PLB.

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The isolation, culture, and plant regeneration of protoplasts derived from callus of Phalaenopsis was established. Callus was obtained by the culture of a lateral bud on a young flower stalk. Protoplasts were isolated enzymatically from callus which was precultured on P basal medium (without coconut water (CW) and sucrose) for 30 days. The first cell division was observed after 7-10 days of culture in the basal medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) or CW. After 60 days of culture, the protoplasts cultured in the medium supplemented with 0. 05-0.

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Potato extract (PE), corn extract (CE) and papaya extract (PAE) significantly enhanced plantlet regeneration from PLBs (protocorm-like bodies) when 50 mL L ) of extract in the medium inhibited the growth of the plantlets. Among these extracts, PE at 100 mL L -1showed the optimum effect on the acceleration of shoot growth while CE at the same concentration was optimum for root growth.