Control of Growth and Development of Protocorm like Body Derived from Callus by Carbon Sources in Phalaenopsis.

Abstract

Protocorm like bodies (PLBs) derived from callus of Phalaenopsis utilized sucrose , maltose and sorbitol for their growth in vitro. These carbon sources affected differently and could control PLB growth . On sucrose supplemented medium a few PLBs produced plantlets and most others regenerated yellowish or greenish callus like body (CLB). Almost 80% of unrooted and 58% of rooted plantlets developed yellowish green CLB at the base of plantlets. On maltose supplemented medium, PLBs regenerated PLBs and a few plantlets. In subsequent culture, about 44% of unrooted and 24% of rooted plantlets initiated green PLBs at the base of cultured plantlets. On sorbitol supplemented medium, most of the PLBs developed plantlets and a few additional PLBs. Among the carbon sources tested, sorbitol supported plantlet development the best in vitro and proved to be the most suitable carbon source for plantlet initiation and development from PLB.

IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

“…var suavis from Bali in the New Phalaenopsis (NP) medium (Islam et al, 1998) added with coconut water and/or tomato juice. The objective of the research was to investigate the effect of coconut water and / or tomato juice on the growth of protocorm of V. tricolor Lindl.…”

Agrobacterium-Mediated Transformation of Indonesian Orchids for Micropropagation

“…A problem in this respect is that seedlings initially form only a single vegetative shoot (Dressler, 1981). However, additional shoots induced from cut protocorm-like bodies (PLBs) can be efficiently obtained using new Phalaenopsis medium, which contains a high concentration of nitrogen (Islam et al, 1998).…”

“…Orchid pods were washed, sterilized three times by dipping in 70% ethanol, flamed, and then put gently on sterilized petri dishes in a laminar air flow hood. Seeds were then sown in New Phalaenopsis (NP) medium (Islam et al, 1998) enriched with 100 g l -1 tomato extract as used by Semiarti et al (2010). Five weeks after sowing, protocorms were collected to be used for target transformation.…”

“…Islam et al (1998) used New phalaenopsis (NP) medium for callus induction of Phalaenopsis. Semiarti et al (2007) also used the NP medium for growing Phalaenopsis orchids before and after genetic transformation treatment using A. tumefaciens.…”

“…Adult plants of P. amabilis (L.) Blume from Java were obtained from Royal Orchids (Prigen, East Java, Indonesia). Seeds were derived from cross-pollinated plants that had been sown on modified, new Phalaenopsis (NP) medium (Islam et al, 1998) and maintained under continuous white light. Adult plants were maintained in a glasshouse at room temperature.…”