Effects of Sucrose, Maltose and Sorbitol on Callus Growth and Plantlet Regeneration in Phalaenopsis, Doritaenopsis and Neofinetia.

Islam, O. M.; Ichihashi, Syoichi

doi:10.2503/jjshs.68.1124

Cited by 22 publications

(13 citation statements)

References 10 publications

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“…Phalaenopsis has highly economical value in flower markets in the world. Therefore, lots of in vitro culture protocols have been developed in this genus (Tanaka et al 1975, Arditti and Ernst 1993, Tokuhara and Masahiro 1993, Ernst 1994, Chen and Piluek 1995, Duan et al 1996, Ishii et al 1998, Islam and Ichihashi 1999, Chen et al 2000, Young et al 2000, Tokuhara and Mii 2001, Park et al 2002. However, only two among them described somatic embryogenesis (Ishii et al 1998, Tokuhara andMii 2001).…”

Section: Introductionmentioning

confidence: 95%

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

Chen¹,

Chang²

2006

Biologia plant.

104

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Leaf explants of Phalaenopsis amabilis var. formosa formed clusters of somatic embryos directly from epidermal cells without an intervening callus within 20 -30 d when cultured on 1/2-strength modified Murashige and Skoog medium supplemented with 0.1, 1 and 3 mg dm -3 TDZ. Repetitive production of embryos involved secondary embryogenesis could be obtained by culturing segments of embryogenic masses on TDZ-containing media. Plantlet conversion from embryos was successfully achieved on regulator-free growth medium.Additional key words: moth orchid, naphthaleneacetic acid, thidiazuron.

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Section: Introductionmentioning

confidence: 95%

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

Chen¹,

Chang²

2006

Biologia plant.

104

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“…Half strength MS medium is also used successfully for orchid culture (Chen and Chang 2000). Carbon sources are critical components in these culture media (Islam and Ichihashi 1999) and sucrose is the most frequently used. However, other sugars used for orchid culture include glucose, fructose, sorbitol and maltose (Islam et al 1998;Islam and Ichihashi 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Carbon sources are critical components in these culture media (Islam and Ichihashi 1999) and sucrose is the most frequently used. However, other sugars used for orchid culture include glucose, fructose, sorbitol and maltose (Islam et al 1998;Islam and Ichihashi 1999). Another component is often the biodegradable polymer chitosan, a deacetylated form of chitin that is present in various organisms, including fungi, crustaceans, insects and some algae.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of the Thai orchid Grammatophyllum speciosum blume

Sopalun

Thammasiri

Ishikawa³

2010

Plant Cell Tiss Organ Cult

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Protocorm-like bodies (PLBs) were induced from shoot tips of Grammatophyllum speciosum, a Thai orchid. The highest frequency of PLBs (93%) were observed on explants incubated on 1/2-Murashige and Skoog (MS) liquid medium containing 2% (w/v) sucrose without any plant growth regulators (PGRs). Tests with different carbon sources compared to sucrose revealed that maltose promoted the highest relative growth of G. speciosum PLBs (7-fold increase), while trehalose and sucrose yielded 5-fold and 4-fold increases, respectively. In 1/2 MS liquid medium, addition of 15 mg/l of chitosan promoted a 7-fold increase in PLB growth while 25 mg/l promoted a 4-fold increase. However, the relative growth rate in solid culture was significantly lower than that in liquid culture. In addition, chitosan supplementation in solid medium promoted shoot formation but not rooting. Plantlet regeneration was induced using a combination of NAA and BA supplementation in 1/2 MS solid medium with optimum induction shoot and root formation at 2.0 mg/l NAA and 1.0 mg/l BA. Using this protocol, approximately 8 months was required to obtain a hundred plantlets from one shoot tip. The plantlets showed no changes in ploidy when tested by flow cytometry.

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“…This genus is very popular and has high economical value in flower markets. A number of in vitro culture protocols have been developed in Phalaenopsis (Tanaka et al, 1975;Arditti and Ernst, 1993;Mii, 1993, 2001;Ernst, 1994;Chen and Piluek, 1995;Duan et al, 1996;Ishii et al, 1998;Islam and Ichihashi, 1999;Chen et al, 2000;Young et al, 2000;Park et al, 2002;Chen and Chang, 2004). However, only two among them described indirect somatic embryogenesis (Ishii et al, 1998;Tokuhara and Mii, 2001).…”

Section: Introductionmentioning

confidence: 99%

Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’

Kuo

Chen

Chang

2005

In Vitro Cell. Dev. Biol. - Plant

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SummaryLeaf segments of the orchid sp. Phalaenopsis 'Little Steve' were used as explants testing the effects of 2,4-dichlorophenoxyacetic acid (2,4-D; 0.45, 2.26, 4.52 mM), 6-furfurylaminopurine (kinetin; 2.32, 4.65, 13.95 mM), N 6 -benzyladenine (BA; 2.22, 4.44, 13.32 mM), and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ; 2.27, 4.54, 13.62 mM) on the induction of direct somatic embryogenesis. After 20 -30 d of culture in darkness, clusters of somatic embryos formed from leaf surfaces and wounded regions of explants on half-strength Murashige and Skoog medium supplemented with BA and TDZ. However, kinetin had no response on direct embryo induction. In addition, 2,4-D highly retarded the frequency of embryogenesis that was induced by TDZ. Generally, adaxial surfaces near wounded regions had the highest embryogenic competency compared to other regions of explants. Histological sections revealed that somatic embryos mostly arose from epidermal cell layers of the explants. Secondary embryogenesis occurred at basal parts of embryos, and originated from outer cell layers. Following transfer of regenerated embryos onto growth regulator-free medium for 3.5-4 mo., plantlets with three to four leaves and several roots were obtained. This protocol provides a simple way to regenerate plants through direct somatic embryogenesis, and is suitable for further studies on embryo development and genetic transformation of Phalaenopsis.

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Effects of Sucrose, Maltose and Sorbitol on Callus Growth and Plantlet Regeneration in Phalaenopsis, Doritaenopsis and Neofinetia.

Cited by 22 publications

References 10 publications

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

Micropropagation of the Thai orchid Grammatophyllum speciosum blume

Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’

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