Effects of Complex Organic Extracts on Plantlet Regeneration from PLBs and Plantlet Growth in the Doritaenopsis Orchid

Rahman, Abu Reza Md. Mahfuzur; Islam, M. O.; ., A.K.M. Azad-ud-doula Prodhan; Ichihashi, Syoichi

doi:10.6090/jarq.38.55

Cited by 16 publications

(11 citation statements)

References 3 publications

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“…The addition of organic additives to the culture medium to promote in vitro growth and proliferation of orchid is a common practice (Arditti and Ernst 1993;Ichihashi and Islam 1999;Chai et al 2002;Islam et al 2003;Rahman et al 2004;Arditti 2008, George et al 2008. The presence of organic additives may contribute toward the development of a simple and economical plant culture media and at the same time minimize the used of exogenous PGRs, thereby possibly reducing the occurrence of undesired somaclonal variation (Lee and Phillips 1988;Islam et al 2003).…”

Section: Proliferation Of Secondary Plbsmentioning

confidence: 98%

In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies

Saleh

2010

Plant Cell Tiss Organ Cult

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Paphiopedilum orchids are among the world's most popular orchid due to their impressively beautiful flowers. Propagation of these orchid genera has been hampered by the naturally slow growth rate of the plant, which renders it very difficult to be propagated through conventional methods. In vitro culture techniques have provided a useful alternative technology for propagating this recalcitrant species. In this study, the propagation of P. rothschildianum was achieved through the in vitro formation of secondary protocorm-like bodies (PLBs) from the primary PLB that developed from stem-derived callus. The PLBs were cultured on half-strength MS medium supplemented with different concentrations (1.0, 2.0, 3.0, and 4.0 lM) of 6-benzyladenine (BA) and kinetin for the induction of secondary PLBs. The highest number of secondary PLBs formed was obtained on half-strength MS medium supplemented with 4.0 lM kinetin, with an average of 4.1 PLBs per explant after 8 weeks of culture. The secondary PLBs continued to proliferate further and formed 9.5-12.1 new PLBs per secondary PLB after being subcultured onto half-strength plant growth regulator-free MS medium supplemented with 60 g/L banana homogenate (BH). These tertiary PLBs were subcultured onto media containing different organic additives, such as BH, coconut water, potato homogenate, and tomato homogenate, for plantlet regeneration. Among the organic additives tested, the addition of 20% CW to half-strength MS medium resulted in the best average plantlet regeneration percentage from the PLBs, 67.9%, after 8 weeks of culture.

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Section: Proliferation Of Secondary Plbsmentioning

confidence: 98%

In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies

Saleh

2010

Plant Cell Tiss Organ Cult

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“…Orange juice, a complex organic extract used in this study contained carbohydrates, protein, several vitamins, lower levels of some amino acids and organic acids [31]. Any of the aforementioned components or other(s) yet unknown substances, alone or in combination might be a factor(s) enhancing plantlet regeneration compared to the control [31]. The present study revealed that 10% -15% orange juice concentrations in the MS media were more suitable for in vitro regeneration and multiplication of cocoyam plantlets.…”

Section: Resultsmentioning

confidence: 99%

Effect of Different Concentrations of Orange Juice for in Vitro Regeneration and Multiplication of Cocoyam (Taro)

Ubalua¹,

Ikpeama²,

Okeagu³

2015

AJPS

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Taro (Colocasia esculenta) and tannia (Xanthosoma sagittifolium) are commonly referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Traditionally, cocoyams are vegetatively propagated from tuber fragments, a practice that encourages pathogen distribution. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. In this study different concentrations of ripped sweet orange (Citrus sinensis) juice were screened for regeneration and multiplication of 2 months old in vitro cocoyam shoot explants. Among the concentrations, maximum numbers of roots (37 ± 5) were observed in Murashige and Skoog (MS) medium supplemented with 10% orange juice after 8 weeks in culture compared to 16 ± 4 (roots) observed in the control medium. On shoot multiplicity, 16 ± 3 shoots were induced in the control medium in contrast to 12 ± 0.8 shoots in the MS medium supplemented with 10% orange juice after 8 weeks of culture. Higher concentrations (25% and 35%) of the orange juice supplemented medium resulted in concomitant inhibition of all the growth parameters. The study successfully established that ripped orange juice could substitute the use of conventional growth hormones cytokinins (BAP) and auxins (NAA) in in vitro regeneration and rapid multiplication of cocoyam shoot explants.

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“…2). Previous studies (Rahman et al 2004) reported that the germination rate of orchid species in vitro is significantly influenced by basal medium used. In this study, the effects of three different basal media on seed germination of Phalaenopsis showed the highest (83.4%) and lowest germination rate (9.4%) on Chen and half MS medium, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze

Balilashaki

Naderi

Kalantari

et al. 2015

Plant Tissue Cult. & Biotech.

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Phalaenopsis orchids have high economic value in the floriculture industry as cut flowers and potted plants throughout the world. Plant tissue culture technology is being widely used for large scale plant multiplication of Phalaenopsis to feed into this industry. In order to increase the efficiency of this technology, four experiments were undertaken: Plantlet regeneration from seeds, nodes or leaves, and hardening of the regenerated plants. In the first experiment, seed germination was examined in three media (half MS, Chen and Vacin-Went) of which the Chen medium had the best result (83.4%) in comparison to the other two media. In the second experiment, nodes on the flower stalk were studied for their shoot formation potential to different concentrations of BA and NAA, The highest frequency of shoot regeneration was achieved on MS containing 4 mg/l BA and 1 mg/l NAA while in vitro derived leaves formed clusters of somatic embryos directly when cultured on MS containing TDZ at different concentrations (0.5, 1, 2 and 3 mg/l). The embryos turned green and developed into protocorm-like bodies after 7 weeks of culture followed by plantlet regeneration. The highest plantlet regeneration from the leaf-derived embryos was obtained from MS supplemented with 3 mg/l TDZ. Finally, regenerated plants from (seeds, nodal explants and leaves) were compared in two medium for hardening, regenerated plant from nodal explants showed the highest survival rate (100%) on the medium containing cocopeat, coal, industrial cartridge and the bites of yonolit (1 : 1 : 2 : 4).

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Effects of Complex Organic Extracts on Plantlet Regeneration from PLBs and Plantlet Growth in the Doritaenopsis Orchid

Cited by 16 publications

References 3 publications

In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies

In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies

Effect of Different Concentrations of Orange Juice for in Vitro Regeneration and Multiplication of Cocoyam (Taro)

Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze

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Effects of Complex Organic Extracts on Plantlet Regeneration from PLBs and Plantlet Growth in the Doritaenopsis Orchid

Cited by 16 publications

References 3 publications

In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies

In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies

Effect of Different Concentrations of Orange Juice for in Vitro Regeneration and Multiplication of Cocoyam (Taro)

Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze

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Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze