Effect of Different Concentrations of Orange Juice for in Vitro Regeneration and Multiplication of Cocoyam (Taro)

Abstract

Taro (Colocasia esculenta) and tannia (Xanthosoma sagittifolium) are commonly referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Traditionally, cocoyams are vegetatively propagated from tuber fragments, a practice that encourages pathogen distribution. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. In this study different concentrations of ripped sweet orange (Citrus sinensis) juice were screened for regeneration and multiplication of 2 months old in vitro cocoyam shoot explants. Among the concentrations, maximum numbers of roots (37 ± 5) were observed in Murashige and Skoog (MS) medium supplemented with 10% orange juice after 8 weeks in culture compared to 16 ± 4 (roots) observed in the control medium. On shoot multiplicity, 16 ± 3 shoots were induced in the control medium in contrast to 12 ± 0.8 shoots in the MS medium supplemented with 10% orange juice after 8 weeks of culture. Higher concentrations (25% and 35%) of the orange juice supplemented medium resulted in concomitant inhibition of all the growth parameters. The study successfully established that ripped orange juice could substitute the use of conventional growth hormones cytokinins (BAP) and auxins (NAA) in in vitro regeneration and rapid multiplication of cocoyam shoot explants.

Effect of types and concentrations of auxins on callus induction and primary somatic embryogenesis in low cyanide cassava cultivars ( <i>Manihot esculentum</i> Cranz)

“…There ficant differences among the concentrations in of callus induction after four weeks of culture. The most appropriate concentrations for callus induction and somatic in these varieties were 4mg/L nd 7mg/L picloram.. A number of studies have shown that picloram and 2,4-D are the growth regulators used most frequently to induce callus formation and embryogenesis Wongtiem et 2011;Ubalua and Mbanaso, 2014). primary somatic embryos r subsequent germination into plantlets requisite to the production of large numbers of plantlets.…”

“…By 28 days after inoculation, significant number of the explants formed callus in all the cassava varieties were significant differences among the genotypes, type and auxin concentrations in the frequency of callus induction after four weeks of culture. The most appropriate concentrations for callus induction and somatic embryogenesis in these varieties were 2,4-D and 7mg/L picloram.. A number of studies have shown that picloram and 2,4 are the growth regulators used most frequently to induce callus formation and embryogenesis in cassava (Hankoua et al, 2006;al., 2011;Ubalua and Mbanaso, 2014). Efficient induction of primary somatic embryos and their subsequent germination into plantlets is a pre-requisite to the production of large in vitro grown cassava genotypes; were used to determine the effect of different concentration callus induction and By 28 days after inoculation, significant number of the explants lus in all the cassava varieties.…”

“…Application of biotechnology in the propagation of cassava has provided a new strategy for mass production of planting materials. A number of protocols has been reported for regeneration of cassava et al, 2006;Hankoua et al, 2006;et al, 2011;Ubalua and Mbanaso, 2014) Successful micropropagation of low cyanide cassava requires optimization of the existing varieties. However, no such had been reported on the varieties cultivated in Nigeria.…”

“…Moreover, convention propagation method allows the generation to another. Application of biotechnology in the propagation of cassava has provided a new strategy for mass production of planting A number of protocols has been regeneration of cassava (Ibrahim, 2006;Wongtiem ., 2011;Ubalua and Mbanaso, 2014). micropropagation of low cyanide requires optimization of protocols for .…”