The isolation, culture, and plant regeneration of protoplasts derived from callus of Phalaenopsis was established. Callus was obtained by the culture of a lateral bud on a young flower stalk. Protoplasts were isolated enzymatically from callus which was precultured on P basal medium (without coconut water (CW) and sucrose) for 30 days. The first cell division was observed after 7-10 days of culture in the basal medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) or CW. After 60 days of culture, the protoplasts cultured in the medium supplemented with 0. 05-0.
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