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Background: Streptozotocin (STZ)-induced free radical oxidant activity resulted in muscle wasting due to protein carbonyl (PC), glucose transporter-4 (Glut-4), and interleukin-6 (IL-6) protein alteration. Antioxidant ingredient in the golden sea cucumber extract was found in promising level to inhibit free radical activity. Aim: This study was aimed to investigate the effect of golden sea cucumber extract on PC, IL-6, and Glut-4 level of STZ-induced diabetes mouse. Materials and Methods: This study was performed using mice, which were grouped into non-diabetes, diabetes, and diabetes-treated extract groups. The golden sea cucumber was extracted using 70% ethanol, which was administered by oral gavage twice a day for 5 consecutive days. Results: The extract reduced PC level and improved muscle Glut-4 and IL-6 protein level of diabetic mouse. Conclusion: The extract of golden sea cucumber revived muscle Glut-4 and IL-6 protein level in protection against muscle wasting.

AbstrakSeiring dengan peningkatan industrialisasi dan transportasi, polusi logam berat yang ditimbulkan pun semakin meningkat. Timbal merupakan toksin yang paling bermakna di antara logam berat lain yang memiliki kecenderungan untuk mengkatalisis reaksi oksidasi dan menimbulkan terbentuknya Reactive Oxygen Species (ROS). Bayam merah (Amaranthus tricolor L) mengandung komponen antioksidan yang berpotensi menurunkan kadar timbal dalam darah dan mencegah toksisitasnya. Penelitian dilaksanakan di Laboratorium Biokimia Fakultas Kedokteran Universitas Airlangga selama bulan Agustus-September 2012. Penelitian eksperimental laboratoris ini menggunakan posttest only control group design. Tiga puluh ekor mencit dibagi menjadi tiga kelompok: K 0 sebagai kelompok kontrol yang diberikan akuades dan plasebo, K 1 sebagai kelompok perlakuan yang diberikan timbal asetat 0,75 mg/10 gBB dan plasebo, K 2 sebagai kelompok perlakuan yang diberikan timbal asetat 0,75 mg/10 gBB dan 382,2 mg/10 gBB jus bayam merah selama 28 hari. Darah diambil dari vena ekor tikus untuk pemeriksaan superoxide dismutase (SOD) dan malondialdehyde (MDA). Hasil pemeriksaan kadar SOD rata-rata darah mencit pada K0 (n=10) 55,91 ± 9,61%, K1 (n=10) 31,29 ± 12,87%, dan K2 (n=10) 34,83 ± 10,12% (p=0,000). Nilai kadar MDA rata-rata darah mencit pada K0 9,9 nmol/mL, K1 12,04 nmol/mL, dan K2 11,05 nmol/mL (p=0,002). Berdasarkan uji ANOVA, disimpulkan bahwa bayam merah dapat meningkatkan SOD dan menurunkan MDA plasma pada toksisitas yang diinduksi timbal asetat pada mencit. The Potency of Red Spinach (Amaranthus tricolor L) as an Antioxidant for Lead-Induced Toxicities in Mice AbstractAlong with the increasing industrialization and transportation, the generated heavy metal pollution is increasing. Lead is the most significant toxin among heavy metals and has a tendency to catalyze the oxidation reaction and lead to the formation of Reactive Oxygen Species (ROS). Red spinach (Amaranthus tricolor L) contains antioxidant properties which have a potential to reduce the blood lead level and prevent the lead-induced toxicities. The study was conducted at the Laboratory of Biochemistry Faculty of Medicine, Universitas Airlangga, during AugustSeptember 2012. This laboratory experimental study used pos-ttest only control group design. Thirty mice were divided into 3 groups: K0 as the control group with aquadest and placebo; K1 as the treatment group with lead acetate 0.75 mg/10 gBW and placebo treatment; and K2 as the treatment group with lead acetate 0.75 mg/10 gBW and 382.2 mg/10 gBW red spinach juice treatment for 28 days. Blood was drawn from the mouse's tail vein for examination of superoxide dismutase (SOD) and malondialdehyde (MDA). Mean blood SOD value in K0 (n=10) was 55.91 ± 9.61%, K1 (n=10) was 31.29 ± 12.87%, and K2 (n=10) was 34.83 ± 10.12% (p=0.000). Mean blood MDA value for K0 was 9.9 nmol/mL, K1 was 12.04 nmol/mL, and K2 was 11.05 nmol/mL (p=0.002). Based on ANOVA test, it is concluded that the administration of red spinach can increase SOD and decrease MDA p...

Introduction: In Indonesia, there has been a 10% increase in the span of five years for exclusive breastfeeding, however this is still far from the national target. One of the factors that can cause low rates of national exclusive breastfeeding is that not all babies have received an early initiation of breastfeeding. The purpose of this study was to determine the relationship between early initiation of breastfeeding and exclusive breastfeeding for babies aged 7-12 months in the work area of Wiyung Public Health Center, Surabaya. Methods: This study was a quantitative research with a cross-sectional approach. The study used a questionnaire as the research instrument given to 30 respondents, namely mothers who had 7-12 month old babies in the work area of Wiyung Public Health Center, Surabaya. Results: The percentage of early initiation of breastfeeding in babies aged 7-12 months was 53.33% meanwhile the percentage of exclusive was 73.33%. Using Chi Square Fisher Exact Test, it was found that there is no relationship between early initiation of breastfeeding and exclusive breastfeeding for babies aged 7-12 months in the working area of the Wiyung Public Health Center in Surabaya. (The value of p = 0.574 with a value of α = 0.05, p>α). Conclusion: The early initiation of breastfeeding is not the only factor influencing exclusive breastfeeding for babies aged 7-12 months in the work area of the Wiyung Public Health Center in Surabaya. Further research is needed on other factors that can relate to and influence exclusive breastfeeding.

This study aims to determine the potency of CV 12 acupuncture as prevention of weight gain in ovariectomizedmice (OVX). Female mice strain BALB/c, 28 mice, 3-4 weeks, 25-35 grams were divided into four groups; namely K1 (n = 8, sham and vehiculum injection), K2 (n = 8, OVX and vehiculum injection), K3 (n = 7, OVX and injection of estrogen intraperitoneal 10 mg/kg/day) and K4 (n = 6, OVX and CV12 acupuncture with a frequency of 2 Hz in 10 mA of continuous spike current wave).Injection and acupuncture given every day for four weeks. Weight change in K1 (0.75 ± 2.8) grams, K2 (2.71 ± 2.4) grams, K3 (0.71 ± 0.95) g and K4 (0.93 ± 2.40) gram with a significant difference in the K2 and K4 (p = 0.011). a receptor expression in K1 (12.25 ± 2.96), K2 (5.43 ± 2.64), K3 (18.86 ± 1.57), K4 (17 ± 2.00) with a significant difference to the K2 K1, K3 and K4 with p<0.05. b receptor expression in K1 (11.38 ± 1.92), K2 (4 ± 1.41), K3 (16.86 ± 1.86), K4 (14.17 ± 5.24) with significantly different a receptor expression in visceral fat mass on the group K2 to K1, K3 and K4 with p<0.05. CV 12 acupuncture increases the expression of a and b estrogen receptor in visceral adipose tissue of OVX mice equivalently and able to prevent the increase of weight due to the estrogen withdrawal. This point could potentially be used as one of the management ofobesity prevention in menopause.

Background: Cardiac arrest is one of the leading cause of death in the world with steadily increasing number over the years. Basic life support has been proven to lower the risks of tissue damage and further complication.Several study shows that BLS skill are lacking in some doctors and nurses. Objective: Therefore, the purpose of this study is knowing the retention of basic life support in medical student of Airlangga University. Methods: This descriptive study used cross sectional design. Subjects were students at faculty of medicine in Airlangga University who trained one year ago and completed their study of Basic Life Support. Fifty two subject were asked to practice their BLS techniques then scored based on modified American Hearth Assosiation (AHA)'s Basic life support skill checklist. Results: Based on the data, the percentage of students who did the retest correctly: response checked by shouting 92.31% students, response checked by tapping 69.23% students, call for help 44.23% students, airway checked 48.08% students, breathing checked 46.15% students, correct hand placement 100% students, compression rate 78.85% students, compression ratio 75% students, position 94.23% students, and compression depth 78.85% students. Conclusion:One year post training and test, 14 from 52 students could complete all the steps in basic life support skill. Less than a half of the students successfully done 3 out of 10 points that were being tested. Meanwhile, many aspects in circulation point show higher results all above 75%. Latar Belakang: Henti jantung merupakan salah atau penyebab kematian tertinggi di dunia dan jumlahnya meningkat dari tahun ke tahun. Basic life support atau bantuan hidup dasar (BHD) terbukti dapat menurunkan kerusakan jaringan dan komplikasi lebih lanjut. Beberapa penelitian menunjukkan ketrampilan BHD pada tenaga medis, baik dokter maupun perawat masih kurang. Tujuan: Melalui penelitian ini dapat diketahui retensi mengenai ketrampilan BHD pada mahasiswa Fakultas Kedokteran Universitas Airlangga. Metode: Penelitian ini merupakan penelitian deskriptif dengan pendekatan cross-sectional. Subjek merupakan mahasiswa Fakultas Kedokteran Universitas Airlangga yang telah menjalani pelatihan BHD dan lulus dalam ujian setelahnya. Lima puluh dua mahasiswa diminta untuk mempraktekkan kembali teknik pemberian BHD dan dinilai menggunakan daftar penilaian modifikasi dari penilaian ketrampilan BHD milik American Heart Association (AHA). Hasil: Persentase mahasiswa yang melakukan dengan benar, antara lain: periksa kesadaran-verbal

Insulin resistance in skeletal muscle is important since it is normally responsible for most of the insulin-mediated glucose disposal and contains the largest glycogen storage regulated by insulin sensitivity. A high-calorie diet is known to be a risk factor for insulin resistance. Lifestyle modification by regulating caloric intake through intermittent fasting (IF) is recommended to prevent insulin resistance. This study aims to analyze the effect of eight weeks of intermittent fasting on skeletal muscle glycogen stores in rats with a four-week high-calorie diet. A total 24 healthy male Wistar rats were divided into four groups, Ad-libitum with a standard diet (AL-SD); Ad-libitum with a high-calorie diet (AL-HCD); IF with a standard diet (IF-SD); IF with a high-calorie diet (IF-HCD). Muscles were obtained and stained with PAS stain to evaluate glycogen expression based on color intensity. The results of the glycogen expression score were obtained as follows, AL-SD=2.113±0.589; AL-HCD=1.827±0.375; IF-SD=2.109±0.499; IF-HCD=1.979±0.334. Although there was no significant difference between groups (p> 0.05), IF can prevent excessive reduction of glycogen stores in subjects with a HCD compared to the AL-HCD group. In conclusion, this study suggests that IF might improve the reduction of glycogen stores in muscles due to a high-calorie diet.