Carnosic acid inhibits the formation of osteoclasts through attenuation of expression of RANKL

Hagiwara, Hiromi; Basnet, Roshan; Wiyasihati, Sundari Indah; Nakata, Kyoko; Hagiwara, Kenta; Miyazaki, Hitoshi; Yoshida, Kaoru

doi:10.1016/j.phanu.2014.11.003

Cited by 6 publications

(8 citation statements)

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“…In addition, there have been some reports that H 2 O 2 suppresses differentiation markers such as alkaline phosphatase activity, type I collagen gene expression, and the mineralization of osteoblastic cells (Hosoya et al 1998 ; Lee et al 2006 ; Mody et al 2001 ). We also reported that carnosic acid attenuated H 2 O 2 levels and osteoblastic differentiation in osteoblastic MC3T3-E1 cells (Hagiwara et al 2015 ). In the present study, 4-HD significantly reduced intracellular H 2 O 2 level of MC3T3-E1 cells.…”

Section: Discussionmentioning

confidence: 66%

“…Quercetin (Notoya et al 2004 ) and curcumin (Notoya et al 2006 ) have been reported to inhibit cultured osteoblast metabolism. In addition, quercetin (Woo et al 2004 ) and carnosic acid (Hagiwara et al 2015 ) have been found to inhibit osteoclastogenesis. Recently, we showed that the olive polyphenols oleuropein and hydroxytyrosol (Hagiwara et al 2011 ) and apigenin (Goto et al 2015 ) inhibit the formation of osteoclasts and attenuate bone loss in OVX mice.…”

Section: Discussionmentioning

confidence: 99%

“…The formation and activation of osteoclasts are controlled by the combined actions of receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Here, we formed multinucleated osteoclasts from splenic cells by co-culture with stromal ST2 cells that had been stimulated by activated vitamin D 3 (Hagiwara et al 2008 , 2015 ; Notoya et al 2007 ). Bone formation involves a complex series of events that include the proliferation and differentiation of osteoprogenitor cells, resulting in the formation of a mineralized extracellular matrix.…”

Section: Introductionmentioning

confidence: 99%

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4-Hydroxyderricin inhibits osteoclast formation and accelerates osteoblast differentiation

et al. 2018

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4-Hydroxyderricin (4-HD) is a major polyphenol of Angelica keiskei (Japanese name Ashitaba), exhibiting anti-allergic, anti-diabetic, anti-oxidant, and antitumor effects. The present study was designed to evaluate the effects of 4-HD on bone formation and maintenance by using cultured osteoclasts and osteoblasts. 4-HD did not affect cell proliferation of stromal ST2 cells and preosteoblast MC3T3-E1 cells at concentrations of 1–10 μM. This compound inhibited the formation of multinucleated osteoclasts from mouse splenic cells, and we identified a molecular pathway of osteoclast differentiation mediated by 4-HD, which led to inhibition of the expression of receptor activator of nuclear factor-κB ligand and macrophage-colony stimulating factor in ST2 cells. By contrast, 4-HD enhanced indices of osteoblast differentiation, such as alkaline phosphatase activity and calcium deposition by osteoblastic MC3T3-E1 cells, at concentrations of 1–10 μM. Furthermore, we found that 4-HD at 1 μM attenuated H 2 O 2 levels in MC3T3-E1 cells. Our findings indicate that 4-HD may have critical effects on bone formation and maintenance.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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4-Hydroxyderricin inhibits osteoclast formation and accelerates osteoblast differentiation

et al. 2018

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“…Carnosic acid is a major bioactive component of rosemary ( Rosmarinus officinalis ) leaves and has been reported to show antioxidant and anti-inflammatory properties [ 43 , 44 , 45 ]. Moreover, it has anti-cancer properties, provides protection to neurons, and regulates cytoprotective responses to oxidative and electrophilic stress as well as enhancing bone formation and inhibiting bone resorption [ 46 ]. The above-cited authors hypothesized that although carnosic acid is degraded in the body, its inhibitory effects on the formation of multinucleated osteoclasts would support the osteoblastic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effects of Fish Oil and Dietary Antioxidant Supplementation on Bone Health of Growing Lambs

Skiba

Raj

Sobol

et al. 2021

Animals

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The aim of the present study was to assess the effects of partial replacement of rapeseed oil (RO) with fish oil (FO) combined with dietary supplementation of various antioxidants on the characteristics of lamb femur. Thirty male lambs were assigned to five dietary treatments and fed isoproteinous and isoenergetic diets for 35 days. The control diet was enriched with 3.0% RO, while the experimental diets were enriched either only with 2.0% RO and 1.0% FO or additionally with 0.1% carnosic acid, 0.1% carnosic acid and 0.35 ppm Se as selenized yeast, or 0.1% carnosic acid and 0.35 ppm Se as sodium selenite. After 35 days, the lambs were slaughtered, and the femur was dissected from the carcass of each animal and analyzed for morphometric, geometric, densitometric, and biomechanical properties. The dietary modifications, specifically the supplementation of FO and selenized yeast, significantly improved the geometric, densitometric, and biomechanical properties of lamb femur.

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“…show antioxidant and anti-inflammatory properties [41,42]. Moreover, it enhanced bone formation and inhibited bone resorption [43]. Above cited authors hypothesised that although carnosic acid is degraded in the body, its inhibitory effects on the formation of multinucleated osteoclasts would support the osteoblastic differentiation.…”

Section: Carnosic Acid Is a Major Bioactive Component Of Rosemary Andmentioning

confidence: 99%

Effects of Fish Oil and Dietary Antioxidant Supplementation on Bone Health

Skiba

Raj

Sobol

et al. 2020

Preprint

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Background: Lambs or sheep are considered an excellent animal model for humans owing to advantages related to bone anatomy, formation, biomechanical characteristics; bone strength; and absorption of minerals and vitamins. Moreover, bone healing in many animal species is faster than that in humans, whereas bone healing, turnover, and remodelling in sheep and humans are comparable. In this context, it would be interesting to examine the effects of bioactive components of diet (including Se and carnosic acid) on bone mineralisation and strength, since these are the most important indicators of bone status. This knowledge may be useful in the context of orthopaedic research, recovery after orthopaedic surgeries, and prevention of skeletal diseases in humans.Results: The aim of the present study was to assess the effects partial replacement of rapeseed oil (RO) with fish oil (FO) combined with dietary supplementation of various antioxidants on the characteristics of lamb femur. Thirty male lambs were assigned to five dietary treatments and fed isoproteinous and isoenergetic diets for 35 days. The control diet was enriched with 3.0% RO, while the experimental diets were enriched either only with 2.0% RO and 1.0% FO or additionally with 0.1% carnosic acid, 0.1% carnosic acid and 0.35 ppm Se as selenised yeast, or 0.1% carnosic acid and 0.35 ppm Se as sodium selenite. After 35 days, the lambs were slaughtered, and the femur was dissected from the carcass of each animal and analysed for morphometric properties.Conclusions: The present study indicated that dietary bioactive components may improve bone health by promoting bone mineralisation in lambs. Partial replacement of RO with FO combined with dietary supplementation of carnosic acid and organic Se improved the geometric, densitometric, and biomechanical properties of lamb femur.

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Carnosic acid inhibits the formation of osteoclasts through attenuation of expression of RANKL

Cited by 6 publications

References 31 publications

4-Hydroxyderricin inhibits osteoclast formation and accelerates osteoblast differentiation

4-Hydroxyderricin inhibits osteoclast formation and accelerates osteoblast differentiation

Effects of Fish Oil and Dietary Antioxidant Supplementation on Bone Health of Growing Lambs

Effects of Fish Oil and Dietary Antioxidant Supplementation on Bone Health

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