Plant diseases have a direct impact on the productivity of crops, and therefore the early detection of diseases is crucial.

The natural occurrence of Tomato spotted wilt virus infecting chrysanthemum was initially detected by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and further confirmed by reverse transcription polymerase chain reaction (RT-PCR) using tospovirus universal primers. The sequence analysis of the PCR products showed 95.6 % and 95.0 of maximum identity at nucleotide and amino acid levels respectively with TSWV chrysanthemum isolate from Ecuador (KT590402). This is the first report of the natural occurrence of TSWV on chrysanthemum in Ecuador.

The serologic survey and the confirmatory molecular diagnostic suggested evidence of a fairly presence of ILT infection in the main poultry farms of Ecuador. The current work highlights the need to apply a control program of ILT based on vaccination and standard biosecurity measures in the poultry farms of Ecuador.

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Peanut bud necrosis virus (PBNV), genus Tospovirus (family Bunyaviridae), is an important virus infecting peanut and other crops in South India. PBNV isolates naturally infecting groundnut, brinjal, tomato, black gram, field bean, cowpea, cotton, jute, taro and Calotropis plants were collected from different regions of South India and characterized. Infection was confirmed by direct antigen‐coating enzyme‐linked immunosorbent assay (DAC‐ELISA) using PBNV‐specific antiserum. The coat protein gene was further amplified using PBNV coat protein‐specific primers. The amplicon (830 bp) was cloned and sequenced; sequence analysis revealed that the N gene shared 93–100% and 95–100% sequence identity with PBNV at the nucleotide and amino acid levels, respectively.

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