The natural occurrence of Tomato spotted wilt virus infecting chrysanthemum was initially detected by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and further confirmed by reverse transcription polymerase chain reaction (RT-PCR) using tospovirus universal primers. The sequence analysis of the PCR products showed 95.6 % and 95.0 of maximum identity at nucleotide and amino acid levels respectively with TSWV chrysanthemum isolate from Ecuador (KT590402). This is the first report of the natural occurrence of TSWV on chrysanthemum in Ecuador.
The serologic survey and the confirmatory molecular diagnostic suggested evidence of a fairly presence of ILT infection in the main poultry farms of Ecuador. The current work highlights the need to apply a control program of ILT based on vaccination and standard biosecurity measures in the poultry farms of Ecuador.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.