The natural occurrence of Tomato spotted wilt virus infecting chrysanthemum was initially detected by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and further confirmed by reverse transcription polymerase chain reaction (RT-PCR) using tospovirus universal primers. The sequence analysis of the PCR products showed 95.6 % and 95.0 of maximum identity at nucleotide and amino acid levels respectively with TSWV chrysanthemum isolate from Ecuador (KT590402). This is the first report of the natural occurrence of TSWV on chrysanthemum in Ecuador.
The serologic survey and the confirmatory molecular diagnostic suggested evidence of a fairly presence of ILT infection in the main poultry farms of Ecuador. The current work highlights the need to apply a control program of ILT based on vaccination and standard biosecurity measures in the poultry farms of Ecuador.
En este estudio se contrastan dos métodos de diagnostico morfológico utilizando como modelo el fitopatógeno Thecaphora solani: tecnicas histológicas con sección y procesamiento de esporas del agente infeccioso y microscopia confocal de estas estructuras. La microscpia confocal demostró proporcionar un nivel de detalle similar al obtenido por técnicas histológicas, con la ventaja adicional de ser una herramienta rápida e igualmente efectiva para este tipo de estudios.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.