Serological and Molecular Identification of Cucumber Mosaic Virus (Cmv) Infecting Banana Crops in Ecuador

Yeturu, Sivaprasad; Méndez, Karla; Garrido, Patricia; Serrano, S Sánchez; Garrido, Ana

doi:10.36331/revista.v3i1.22

Cited by 2 publications

(3 citation statements)

References 4 publications

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“…The positive reaction of all the virus isolates with cucumovirus polyclonal antiserum further affirmed they belong to the genus Cucumovirus.The production of 500 base pair amplicons using cucumovirus specific primers by all the virus isolates in this study further confirmed that they were all CMV isolates. Yeturu, et al [33] had reported the detection of CMV using cucumovirus specific primers with the resultant production of amplicons of 500 base pairs. Characterization and identification of plant viruses using gene sequence data has become the ultimate in recent times [34][35][36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Biological, Serological and Molecular Characterization of Cucumber mosaic virus (CMV) Infecting Cucurbita moschata (Duchene ex. Poir) (Summer Squash) in Itu and Uyo Local Governments Areas, Akwa Ibom State, Nigeria

Usanga,

Owolabi,

Eyong

2024

AJBGMB

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Cucurbita moschata is an edible member of the family Cucurbitaceae. The plant is commonly observed showing typical virus-like symptoms during the growing season in Akwa Ibom State, Nigeria. A total of 7 leaf samples showing typical virus-like symptoms were collected from C. moschata in different locations in Itu and Uyo Local Government, Akwa Ibom State. All the virus isolates used in this study were sap transmissible with a wide host range. The virus isolates irrespective of the place of collection were transmitted in a fore-gut manner by A. spiraecola. Aphis citricida did not transmit the viruses. All the virus isolates reacted positively to cucumovirus polyclonal antibody in antigen coated plate-ELISA (ACP-ELISA). Gel electrophoresis of the cDNA of the virus isolates produced 500 base pairs typical of Cucumber mosaic virus. The gene sequence analysis revealed that all the isolates had sequence homology of 95% and above with Cucumber mosaic virus (CMV) and are therefore considered strains of CMV. The results also showed that CMV isolates investigated in this study shared common ancestry, though belonging to different clusters. The results also indicated that CMV could be widespread and therefore a threat to C. moschata production in Itu and Uyo Local Government Areas of Akwa Ibom State. This is the first report of CMV infecting C. moschata in Akwa Ibom State, Nigeria. Further work should be carried out to cover the entire state.

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Section: Discussionmentioning

confidence: 99%

Biological, Serological and Molecular Characterization of Cucumber mosaic virus (CMV) Infecting Cucurbita moschata (Duchene ex. Poir) (Summer Squash) in Itu and Uyo Local Governments Areas, Akwa Ibom State, Nigeria

Usanga,

Owolabi,

Eyong

2024

AJBGMB

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“…Mosaic patterns or discontinuous linear streaking bands, extending from leaf margin to midrib are the characteristic symptoms of infectious chlorosis. Curling of leaves, rosette appearance of leaf arrangement and dead or dying suckersare noticed in advanced cases (Niblett et al, 1994;Rodoni et al, 1997;Sivaprasad et al, 2016;Tripathy, 2016). Banana mosaic is categorised as cosmopolitan and is found wherever bananas are grown.…”

Section: Symptoms Developed By the Virusmentioning

confidence: 99%

“…Cucumber mosaic virus isolates were phylogenetically analysed and the subgroup I have subdivided into IA and IB. Among the subgroups, subgroup 1B of CMV is limited to Asia, and the other two subgroups (i.e., 1A and 2) are distributed worldwide (Sivaprasad et al, 2016).…”

Section: Distribution Of the Virusmentioning

confidence: 99%

The Virus Causing Infectious Chlorosis in Banana (Musa sp.): A Review

Antony¹,

Shaana²,

Visakh³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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Banana fruit crops are high in potassium, contain a high level of protein and dietary fiber which makes it world's most popular. The crop gives a maximum return to the farmers when good cultivation practices are followed, which include using diseasefree planting material. The crop is infected by several viruses viz., Banana bunchy top virus, Cucumber mosaic virus, Banana streak virus, Banana bract mosaic virus and Banana mild mosaic virus (Tripathi et al., 2016). Among these viruses, CMV causes a devastating effect on tissue culture banana plants. Various symptoms of CMV were reported under natural conditions like diamond-shaped discontinuous lesions, severe mosaic with extreme distortion and reduction of leaf lamina. KAU (2016) opined that infection due to CMV was observed in widely cultivated banana varieties in Kerala, such as Nendran, Palyankodan, Karpooravally and Poovan (Rasthali). Hence, virus indexing is a must to select healthy planting materials. Cucumber mosaic virus is transmitted through more than 60 species of aphids, including Aphis gossypii and Myzuspersicaeetc. The serological methods are widely used in the detection of Cucumber mosaic virus from the field.This review paper is focused on various aspects of novel detection methods of CMV infecting banana.

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Serological and Molecular Identification of Cucumber Mosaic Virus (Cmv) Infecting Banana Crops in Ecuador

Cited by 2 publications

References 4 publications

Biological, Serological and Molecular Characterization of Cucumber mosaic virus (CMV) Infecting Cucurbita moschata (Duchene ex. Poir) (Summer Squash) in Itu and Uyo Local Governments Areas, Akwa Ibom State, Nigeria

Biological, Serological and Molecular Characterization of Cucumber mosaic virus (CMV) Infecting Cucurbita moschata (Duchene ex. Poir) (Summer Squash) in Itu and Uyo Local Governments Areas, Akwa Ibom State, Nigeria

The Virus Causing Infectious Chlorosis in Banana (Musa sp.): A Review

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