First report of tomato spotted wilt virus infecting Chrysanthemum in Ecuador

Abstract

The natural occurrence of Tomato spotted wilt virus infecting chrysanthemum was initially detected by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and further confirmed by reverse transcription polymerase chain reaction (RT-PCR) using tospovirus universal primers. The sequence analysis of the PCR products showed 95.6 % and 95.0 of maximum identity at nucleotide and amino acid levels respectively with TSWV chrysanthemum isolate from Ecuador (KT590402). This is the first report of the natural occurrence of TSWV on chrysanthemum in Ecuador.

Immunocapture of virions with virus-specific antibodies prior to high-throughput sequencing effectively enriches for virus-specific sequences

“…The virus was later renamed potato yellowing virus [29, 30, 31]. In subsequent studies, PYV was reported on potato and pepper plants from Chile and Ecuador with only partial sequence information provided [32, 33] covering less than 1 kb of the genome. Our PYV isolate (PV-0706) was obtained from a pepino fruit ( Solanum muricatum ) of unknown geographic origin purchased in a German supermarket and was propagated on D .…”

Occurrence of potato yellowing virus in naranjilla (Solanum quitoense Lam.) in Ecuador

“…The RNA dependent RNA polymerase gene (RdRp) was used for a phylogenetic analysis using BEAST under the LG+G model and a strict molecular clock. The phylogeny showed that the PYV isolate from naranjilla clustered with an isolate described in pepper from the same location in Tumbaco (KP772263) (Sivaprasad et al 2015), but was distantly related with isolates from Solanum phureja from other locations in Ecuador (Silvestre et al 2011) Moreover, the PYV RNAs have 80.1, 78.1 and 73.5 identity with Fragaria chiloensis latent virus, (NC_006566, NC_006567, NC_006568) which confirms that PYV as a distinct Ilavirus (family Bromoviridae). To our knowledge, this is the first report of PYV infecting S. quitoense as a new host.…”

“…Starting in 2015, generalized chlorosis, inter veins-space yellowing or chlorotic areas and young leaf curling symptoms were detected in plants of S. quitoense in Tumbaco, Pichincha, in plants of common origin at the INIAP Experimental Farm, Ecuador. The occurrence of potato yellowing virus (PYV) was confirmed in symptomatic leaves, from INIAP experimental farm, by DAS-ELISA with PYV specific antiserum (Sivaprasad et al 2015), eight out of ten plants tested positive. High throughput sequencing (HTS) was carried out on a pool of RNA from leaves of three different symptomatic plants .…”

Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (Solanum lycopersicum)

“…S RNA is a double-sense and antisense RNA that encodes nucleocapsid proteins (N) and a sense RNA that encodes non-structural proteins (NSs) [12]; both types of proteins play crucial roles in the TSWV infection cycle [13][14][15]. The TSWV has an extremely wide range of susceptible hosts, including many important agricultural and field crops (infecting more than 1090 plant species), especially tomato, pepper, potato, and tobacco [16][17][18][19][20]. TSWV diseases are widely distributed, and the most severe cases are found in temperate and subtropical areas (Figure 1).…”