Research on new antimicrobial agents is needed, as more and more microorganisms that cause antibiotic-resistant diseases are emerging commercially. In this group, we can find strains of Staphylococcus aureus and Escherichia coli, which are highly opportunistic species. Faced with this perspective, research using essential oils present in plants is emerging as a therapeutic alternative for the treatment of antimicrobial infections. Many of these oils have, in their composition, monoterpene α-pinene, that shows to have antibacterial activity. The purpose of this research is to evaluate the antimicrobial activity of the positive enantiomer of α-pinene against strains of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The methodologies used were: Disc-diffusion test, broth microdilution and bacterial death kinetics, all of which were standardized by CLSI. As a result, inhibition halos of 11 mm was obtained for the gram-positive strain and 12 mm for the gram-negative strain, both at the same concentration, 160 µl / ml. In addition, it was possible to observe with the death curve that the concentrations (1.25 µl/mL and 2.5 µl/mL of the (+)-α-pinene were able to eliminate the formation of bacterial colonies at one time of exposure of 2 hours for the E. coli strain. However, the death curve of the S. aureus strain was characterized by non-elimination of bacterial colonies at a 24 hours exposure time used for the experiment. Only amikacin evidenced its bacterial killing rate of all colonies within two hours of exposure. At the end, it was possible to verify the activity of the phytoconstituent against Escherichia coli strains ATCC 25922 and Staphylococcus aureus ATCC 25923, recommending the continuity of the studies with the use of different methodologies so that (+) - α-pinene in the future can be a compound used in antimicrobial therapy.

Introduction: This increase in the prevalence of drug-resistant pathogens occurs at a time when the discovery and development of new antimicrobial agents occur slowly. In this context, the objective of this study was to investigate the antifungal activity of isoeugenol, a phenylpropanoid, by in vitro and in silico assays against Penicillium citrinum strains. Material and Method: For in silico analysis, the software PASS online, Molinspiration and Osíris were used. For the determination of Minimum Inhibitory Concentration (MIC) and Minimal Fungicide Concentration (MFC) of isoeugenol and voriconazole were carried out using the broth microdilution technique. PASS online has shown that isoeugenol has the opportunity to present antiseptic, antifungal, antibacterial, antimycobacterial activities. Molinspiration showed that the phytoconstituent has good potential for oral bioavailability. Conclusion: In the analysis with the Osiris program, it was demonstrated that isoeugenol has low irritant and tumorigenic risk. The MIC of isoeugenol varied between 256 and 32 µg/mL, MIC50 of 64 µg/mL and MIC90 was 128 µg/mL. The MFC50, MFC90 and MFC of the isoeugenol for P. citrinum species were 64, 256 and 518 μg/mL, respectively. After analysis, it was verified that the isoeugenol have bactericidal effect against the strains of P. citrinum. After these results, it is important to discover the mechanism of action involved in the antifungal action of the compound, as well as in vitro and in vivo toxicity tests.

Palavras-chave: Pterodon emarginatus, Plantas medicinais, Óleos essenciais.ABSTRACT: Evaluation of the antimicrobial activity of the essential oil of sucupira (Pterodon emarginatus). The use of plants for therapeutic purposes is one of the oldest forms of medical practice. This has aroused scientific interest through studies on the antimicrobial activity of extracts and essential oils from plants for the purpose of creating alternatives to combat microorganisms resistant to antibiotics. The objective of this study was to evaluate the potential antimicrobial activity of the essential oil of Pterodon emarginatus against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922. The essential oil of P. emarginatus was extracted by the method of hydrodistillation by steam distillation using a modified Clevenger apparatus. Antimicrobial activity was determined using the method of diffusion in solid medium by the pour plate technique using pure essential oil (100%) at the following concentrations: 32%, 16%, 8%, 4%, 2%, 1% and 0.5%. None of the concentrations formed zones of inhibition for Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922. The present findings suggest that the essential oil of P. emarginatus has no antimicrobial activity against S. aureus. P. aeruginosa and E. coli.

<p class="COcorpodoresumoCxSpFirst">Discorremos sobre as diferentes práticas pedagógicas que perpassam o projeto de extensão “Auto-organização de mulheres e saúde”, tendo em vista que a educação popular torna-se componente primordial e indispensável na realização das oficinas do projeto. Nestas práticas destacamos a utilização de recursos didáticos e metodologias como importantes instrumentos motivadores, facilitadores da aprendizagem em saúde. Desenvolvemos levantamento bibliográfico, análise dos relatórios de vigências anteriores do projeto, e análise da experiência da oficina “Saúde da mulher, corpo e sexualidade”. Abordamos a discussão acerca das práticas pedagógicas e da importância da educação popular em saúde. Concluímos que as práticas pedagógicas que norteiam as oficinas promovidas pelo projeto propiciam a todas as envolvidas a construção de saberes, tomando como ponto de partida o planejamento e as ações educativas sobre os temas abordados nas oficinas.</p><p class="COcorpodoresumoCxSpMiddle"> </p><p class="COcorpodoresumoCxSpLast">PALAVRAS-CHAVE: Educação popular; práticas pedagógicas; saúde.</p>

Background: The increasing and inappropriate use of antibiotics has increased the number of multidrug-resistant microorganisms to these drugs, causing the emergence of infections that are difficult to control and manage by health professionals. As an alternative to combat these pathogens, some monoterpenes have harmful effects on the bacterial cell membrane, showing themselves as an alternative in combating microorganisms. Therefore, the positive enantiomer α-pinene becomes an alternative to fight bacteria, since it was able to inhibit the growth of the species Escherichia coli ATCC 25922, demonstrating the possibility of its use as an isolated antimicrobial or associated with other drugs. Aims: The aim of this study is to evaluate the sensitivity profile of E. coli ATCC 25922 strain against clinical antimicrobials associated with (+) -α-pinene and how it behaves after successive exposures to subinhibitory concentrations of the phytochemicals. Methods: The minimum inhibitory concentration (MIC) was determined using the microdilution method. The study of the modulating effect of (+) -α-pinene on the activity of antibiotics for clinical use in strains of E. coli and the analysis of the strain's adaptation to the monoterpene were test using the adapted disk-diffusion method. Results: The results demonstrate that the association of monoterpene with the antimicrobials ceftazidime, amoxicillin, cefepime, cefoxitin and amikacin is positive, since it leads to the potentiation of the antibiotic effect of these compounds. It was observed that the monoterpene was able to induce cross-resistance only for antimicrobials: cefuroxime, ceftazidime, cefepime and chloramphenicol. Conclusion: It is necessary to obtain more concrete data for the safe use of these combinations, paying attention to the existence of some type of existing toxicity reaction related to the herbal medicine and to understand the resistance mechanisms acquired by the microorganism.

RESUMOOs universitários são uma classe de jovens que possui uma realidade muito específica, por causa do ambiente acadêmico e fatores sociais, e vem chamando a atenção de muitos pesquisadores por apresentarem um aumento significativo no desenvolvimento das doenças neuropsíquicas, em destaque a depressão, que em casos graves levam ao suicídio. Essas enfermidades estão cada vez mais provocando um impacto na saúde mundial. Esse trabalho tem como objetivo identificar os fatores associados ao suicídio em universitários, por meio de uma pesquisa bibliográfica realizada em julho de 2017, com artigos científicos em inglês, português e espanhol, sendo selecionados 11 para compor o estudo. Os resultados encontrados foram que fatores intrínsecos a instituição de ensino superior como também fatores pessoais podem causar a depressão evoluindo para o suicídio desses estudantes. Portanto, é de extrema importância a identificação desses fatores pois assim será possível a realização de medidas de auxílio e prevenção pelas instituições de ensino superior, para abrandar e posteriormente sanar essa enfermidade social que vem destruindo a vida de muitos jovens. PALAVRAS-CHAVE: Suicídio; universitários; fatores. BREAKING TABOOS: THE FACTORS THAT LEAD TO SUICIDE AMONG COLLEGE STUDENTS.ABSTRACT University students are a class of young people who have a very specific reality, because of the academic environment and social factors, and has attracted the attention of many researchers for presenting a significant increase in the development of neuropsychic diseases, in particular depression, which in cases Lead to suicide. These diseases are increasingly having an impact on global health. This work aims to identify the factors associated with suicide in university students, through a bibliographic research conducted in July 2017, with scientific articles in English, Portuguese and Spanish, being selected 11 to compose the study. The results found were that factors intrinsic to the institution of higher education as well as personal factors can cause depression evolving to the suicide of these students. Therefore, it is

Aims:The hospital environment can act as a reservoir for microorganisms, which in turn can contaminate a range of hospital equipment and survive for long periods of time. One of these environments the Neonatal Intensive Care Unit (NICU), for the initial post partum period serving as a home for newborns of low birth weight and needing invasive procedures for administration of nutritional and medicinal substances, which makes the NICU a critical area for housing individuals with immune system. Thus, the aim of this study was to evaluate the phenotypic appearance of resistance of the Staphylococcus spp. compared to erythromycin and clindamycin, originating from

OBJETIVO: Propor reflexividade a respeito do uso de fitoterápicos em saúde mental sob à luz da teoria de Orem. MÉTODO: Consiste em um estudo descritivo-exploratório, com abordagem qualitativa e reflexiva, utilizando documentação indireta. A pesquisa foi realizada na Biblioteca Virtual em Saúde, utilizando os descritores fitoterápicos; saúde mental. Ainda foi realizado uma investigação complementar com base na teoria do autocuidado proposta por Orem. A obra foi estruturada com 26 obras, que respondiam aos critérios propostos, nos idiomas inglês, português e espanhol. RESULTADOS: A saúde mental é uma área que vem sendo cada vez mais explorada e discutida. Na atualidade, as emoções estão sendo classificadas socialmente cada vez mais como doenças, necessitando de medicalização. O uso de fitoterápicos com a finalidade psicomoduladora através da automedicação é cada vez mais realizado, exaltando a problemática da banalização desses medicamentos e da dificuldade de enfrentamento, fragilizando cada vez mais a saúde mental. A teoria do autocuidado propõe o desenvolvimento de ações apoio-educativas que instruam a população para boas práticas em saúde, contornado essa realidade. CONCLUSÃO: O trabalho de Orem guia a aplicação de uma terapêutica que auxilie os clientes na manutenção da saúde mental, com o enfrentamento das emoções, e na utilização adequada de fitoterápicos. Para tanto é necessário a implementação de atividades educativas, para a população e profissionais da saúde, especialmente enfermeiros de Estratégia de Saúde da Família. Assim, os pacientes serão mais empoderados quanto a saúde e os profissionais terão subsídio para capacitar seu público e prescrever adequadamente a fitoterapia. Palavras chave: Autocuidado; Fitoterapia; Saúde Mental.