Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been
used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research
were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and
correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different
polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay.
Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and
its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane,
ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH 50 g/ml and it can be classified as
very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g)
and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was
given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative
correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were
contributor major in its antioxidant activities by DPPH method.
Introduction:The Morus nigra (Moraceae) known as black mulberry is a well-grown plant in Lembang, Indonesia. Previous studies showed that black mulberry fruit has activity as antibacterial and antioxidant. The aims of this study were to evaluate the antibacterial effect of ethanol extract from stem bark, fruit, and leaves of Morus nigra, MIC and morphological changes of the most active extract against Staphylococcus epidermidis and Propionibacterium acnes. Methods: Agar diffusion and microdilution assays were used to evaluate the antibacterial effect of the extract by measuring the zone of inhibition and the minimum inhibitory concentration (MIC) of the extract against the test bacteria. Scanning electron microscopy (SEM) was performed to evaluate the morphological changes induced by the extract in cellular membrane of the bacteria. Protein and ion leakage from the bacterial cells induced by the extract were also evaluated. Results: The antibacterial activity showed the most active extract was an ethanol extract of the stem bark against S. epidermidis and P. acnes with MIC value 4 mg/ml and 2 mg/ml, respectively. The extract also induced protein and ion leakage in test bacterial cells. SEM findings revealed that the extract induced potential cellular membrane damage in 4xMIC. Conclusion: The antibacterial activity was related to the damaged of bacterial cell wall.
<p>Umbi bawang dayak (<em>Eleutherine palmifolia (L.)</em> Merr.) memiliki kandungan fenol dan flavonoid yang berkhasiat sebagai antioksidan. Kandungan kimia dalam suatu tumbuhan dapat dipengaruhi dengan adanya proses pengolahan bahan baku. Salahsatu proses pengolahan baku tumbuhan yaitu dengan adanya fermentasi menggunakan pemanasan. Penelitian ini bertujuan mengetahui pengaruh fermentasi pada kandungan kimia dan aktivitas antioksidan pada ekstrak umbi bawang dayak. Penelitian ini dilakukan dengan fermentasi umbi bawang dayak dengan pemanasan selama 10, 20 dan 30 hari. Ekstraksi dilakukan menggunakan maserasi dengan pelarut etanol 70%. Penetapan kadar fenol total, flavonoid total dan aktivitas antioksidan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan, ekstrak umbi bawang dayak hasil fermentasi selama 30 hari memiliki kadar fenol total, kadar flavonoid total dan aktivitas antioksidan tertinggi, ditunjukkan secara berurutan (3,721 g GAE/100 g ekstrak ± 0,210; 0,378 g QE/100 g ekstrak ± 0,189, IC<sub>50</sub> peredaman DPPH 28,689 µg/mL± 0,144). Adanya proses fermentasi dan lama proses fermentasi dapat mempengaruhi kandungan kimia dan aktivitas antioksidan ekstrak umbi bawang dayak.</p>
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.