Tomato yellow leaf curl virus disease (TYLCVD) has been observed in Tunisia for more than 20 years. Until year 2004, only the Tomato yellow leaf curl Sardinia virus-Sicily (TYLCSV-[Sic]) was detected in tomato, pepper and bean crops. In the Sahel region, some tomato samples showing severe TYLCVD symptoms were collected from greenhouses in 2004 and 2005. Typing of these isolates revealed for the first time the presence of the TYLCV Israel in Tunisia. This result was confirmed by using several sets of specific primers and by sequencing. This species has also been detected on pepper and bean collected from fields in the same region. The sequencing of a tomato and a bean isolate showed that they both share more than 97% of sequence identity with the TYLCV from Dominican Republic (AF024715). The TYLCV has been found in single and mixed infection with the TYLCSV-[Sic].www.blackwell-synergy.com
The full-length genome of a Tunisian isolate of Tomato yellow leaf curl Sardinia virus (TYLCSV) was engineered and submitted to sequence analysis. The Tunisian isolate has 99% sequence identity with TYL-CSV-Sicilian (Sic), results thus providing further evidence for the inclusion of this isolate in the TYL-CSV-Sic group. A 1.7-mer construct of the virus was obtained and efficiently agroinfiltrated into tomato and tobacco plants to induce symptoms indistinguishable from those of natural infection.www.blackwell-synergy.com
Tomato yellow leaf curl disease (TYLCD) and salinity stress adversely affect tomato production worldwide by causing extensive damages. In Tunisia, identifying TYLCD resistant cultivars selected in different environments is useful to devise counter-measures. To this end, 20 tomato commercial cultivars were screened for different Ty gene alleles' combinations and evaluated either for TYLCD incidence or salinity constraint. We built a biological multi-layer network for integrating, visualizing and modelling generated data. It is a simple representation view linking allelic combinations to tomato cultivars behaviour under viral and salt stresses. In addition, we analyzed differential expression of transcriptions factors (TFs) belonging to WRKY and ERF families in selected resistant (R) and susceptible (S) tomato cultivars. Gene expression was evaluated for short-and long stress exposure to either TYLCSV infection or to both viral and salinity stresses. Evidence is that TFs promote resistance to abiotic and biotic stresses through a complex regulatory network.
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