PCR-Based Methods for Tagging the Mi-1 Locus for Resistance to Root-Knot Nematode in Begomovirus-Resistant Tomato Germplasm

Mehrach, Khadija El; Chouchane, S. Gharsallah; Mejía, Luis C.; Williamson, Valerie M.; Vidavski, F.; Hatimi, Abdelhakim; Salus, Melinda S.; Martin, Christopher T.; Maxwell, D. P.

doi:10.17660/actahortic.2005.695.29

Cited by 35 publications

(14 citation statements)

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“…Allele 2 was present in SC, introgressed from S. chilense. Allele 3 was shown by LA3473;El Mehrach et al (2005) found 2 TaqI restriction sites in the amplification product of the REX-1 locus of this accession, which would result in the three bands observed in our results. LA3474 is a breeding line derived from an initial cross between S. lycopersicum cv.…”

Section: Discussionsupporting

confidence: 45%

“…None of the S. lycopersicum plant materials without Mi showed allele 2, except for SC, which carried Ty-1 but not Mi. Furthermore, it has been reported that begomovirus-resistant lines derived from S. habrochaites that are susceptible to M. incognita give false positive results for the REX-1 marker (El Mehrach et al, 2005). These authors studied the REX-1 marker at the sequence level, detecting the same single nucleotide polymorphism (SNP) associated with a TaqI restriction site in plant material with introgressions from S. peruvianum and S. habrochaites.…”

Section: Discussionmentioning

confidence: 99%

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Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

et al. 2007

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During the process of breeding programmes, several resistance genes have been introgressed into tomato (Solanum lycopersicum) cultivars from different wild tomato relatives. A number of these resistance genes have been mapped to chromosome 6. Among them, Ty-1 and Mi, which confer resistance to Tomato yellow leaf curl disease and to Meloidogyne spp., respectively, are in most cases incorporated in commercial hybrids. Several molecular markers tightly linked to Mi have been identified. This study was conducted in order to find an informative molecular marker linked to Ty-1. Six markers mapped in the same region as Ty-1 were analysed in plant material carrying different combinations of Ty-1 and Mi alleles. Three of the six markers revealed polymorphism among the assayed accessions. One allele of JB-1 marker showed association with Ty-1. Furthermore, the presence of Mi did not interfere with the results. The analysis of several accessions of wild tomato relatives with the three polymorphic markers allowed the establishment of the origin of the alleles found in cultivated plant material, showing that introgressions from S. lycopersicum, S. pimpinellifolium and S. habrochaites will not interfere with the results of this marker which tags Ty-1. Furthermore this analysis enabled the location of CT21, the RFLP marker from which JB-1 was designed.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

et al. 2007

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“…From these markers, the co-dominant marker PMiF3-R3 (El Mehrach et al 2005, Table 1) fits best to the requirement of a routine application, i.e. no a-specific bands and a clear separation of resistant (R) and susceptibility (S)-specific bands on agarose gels.…”

Section: Resultsmentioning

confidence: 91%

Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing

Arens¹,

Mansilla

Deinum³

et al. 2009

Theor Appl Genet

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Molecular markers linked to phenotypically important traits are of great interest especially when traits are difficult and/or costly to be observed. In tomato where a strong focus on resistance breeding has led to the introgression of several resistance genes, resistance traits have become important characteristics in distinctness, uniformity and stability (DUS) testing for Plant Breeders Rights (PBR) applications. Evaluation of disease traits in biological assays is not always straightforward because assays are often influenced by environmental factors, and difficulties in scoring exist. In this study, we describe the development and/or evaluation of molecular marker assays for the Verticillium genes Ve1 and Ve2, the tomato mosaic virusTm1 (linked marker), the tomato mosaic virus Tm2 and Tm22 genes, the Meloidogyne incognita Mi1-2 gene, the Fusarium I (linked marker) and I2 loci, which are obligatory traits in PBR testing. The marker assays were evaluated for their robustness in a ring test and then evaluated in a set of varieties. Although in general, results between biological assays and marker assays gave highly correlated results, marker assays showed an advantage over biological tests in that the results were clearer, i.e., homozygote/heterozygote presence of the resistance gene can be detected and heterogeneity in seed lots can be identified readily. Within the UPOV framework for granting of PBR, the markers have the potential to fulfil the requirements needed for implementation in DUS testing of candidate varieties and could complement or may be an alternative to the pathogenesis tests that are carried out at present.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-009-1183-2) contains supplementary material, which is available to authorized users.

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“…Em geral, para isso, são realizados vários cruzamentos e posterior fenotipagem dos indivíduos obtidos por meio de inoculações. Para diminuir essas dificuldades em fenotipar e selecionar os indivíduos desejados, principalmente quanto à resistência às doenças de natureza virótica (Menezes et al, 2004;El Mehrach et al, 2005), a identificação de marcadores moleculares ligados a alelos de resistência é um dos principais objetivos dos atuais programas de melhoramento para a cultura do tomateiro (Barone, 2009). A identificação de alelos de resistência a geminivírus, associados a marcadores moleculares, pode contribuir para estabelecer melhores estratégias de melhoramento e seleção de genótipos resistentes.…”

Section: Introductionunclassified

Marcador microssatélite associado ao alelo Ty-1 de resistência a Begomovirus em tomateiro

Nogueira

Maluf²,

Nogueira

et al. 2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi associar um marcador microssatélite ao alelo Ty-1 de resistência a Begomovirus em tomateiro, e avaliar a eficiência desta associação na seleção de linhagens resistentes ao vírus. Os marcadores SSR-47 e SSR-48 foram testados em linhagens isogênicas contrastantes quanto à presença do alelo Ty-1 (LA-3473, LA-3474, LA-3475). O marcador SSR-47, por ter detectado polimorfismo nas linhagens, foi o único utilizado nas etapas subsequentes da pesquisa. Detectada a associação entre o SSR-47 e o alelo Ty-1, testou-se sua eficiência na seleção de genótipos avançados de tomateiro. Para confirmar a eficiência da seleção, foi realizada a avaliação fenotípica das plantas com padrões contrastantes de bandas para SSR-47, quanto à resistência a Begomovirus. Plantas que apresentaram banda única de 191 pb foram resistentes ao Begomovirus, pelo teste de inoculação por enxertia; aquelas com banda única de 180 pb foram suscetíveis; e as plantas com bandas de 191 e 180 pb foram resistentes. A distância máxima entre o Ty-1 e o marcador SSR-47 foi de 2,7 cM. Este marcador foi efetivo em caracterizar genótipos portadores do alelo Ty-1. As respostas das plantas à infecção pelo Begomovirus, induzida via enxertia, são consistentes com as reações previstas com o uso do marcador molecular SSR-47.Termos para indexação: Geminiviridae, Lycopersicon esculentum, Solanum lycopersicum, QTL, seleção assistida por marcadores. Microsatelite marker associated with the Ty-1 allele for resistance to Begomovirus in tomatoAbstract -The objective of this work was to associate a microsatellite marker with the Ty-1 allele that controls resistance to Begomovirus in tomatoes, and to assess the eficiency of this association in the selection of virus-resistant lines. Microsatellite markers SSR-47 and SSR-48 were tested in near isogenic tomato lines (LA-3473, LA-3474, LA-3475) with contrasting genotypes for the Ty-1 allele. The marker SSR-47 detected polymorphism in the lines, and was the only one used in the subsequent research phases. An association between the SSR-47 marker and the Ty-1 locus was detected, and its efficiency for selection of begomovirus-resistant genotypes in tomato was assessed. To confirm the selection efficiency, plants with contrasting banding patterns for the SSR-47 marker were tested for their reaction to Begomovirus. All plants with a single 191-bp band were resistant to Begomovirus, through inoculation test by grafting. Plants with a single 180-bp band were susceptible, and plants with both 191bp and 180 bp bands were resistant. The maximum distance between Ty-1 and the SSR-47 marker was 2.7 cM. This marker was efficient to identify genotypes bearing the Ty-1 allele. Plant responses to infection by Begomovirus inoculated through grafting were consistent with reactions predicted with the use of the SSR-47 marker.

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PCR-Based Methods for Tagging the Mi-1 Locus for Resistance to Root-Knot Nematode in Begomovirus-Resistant Tomato Germplasm

Cited by 35 publications

References 0 publications

Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing

Marcador microssatélite associado ao alelo Ty-1 de resistência a Begomovirus em tomateiro

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