Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato

Abstract

During the process of breeding programmes, several resistance genes have been introgressed into tomato (Solanum lycopersicum) cultivars from different wild tomato relatives. A number of these resistance genes have been mapped to chromosome 6. Among them, Ty-1 and Mi, which confer resistance to Tomato yellow leaf curl disease and to Meloidogyne spp., respectively, are in most cases incorporated in commercial hybrids. Several molecular markers tightly linked to Mi have been identified. This study was conducted in order to find an informative molecular marker linked to Ty-1. Six markers mapped in the same region as Ty-1 were analysed in plant material carrying different combinations of Ty-1 and Mi alleles. Three of the six markers revealed polymorphism among the assayed accessions. One allele of JB-1 marker showed association with Ty-1. Furthermore, the presence of Mi did not interfere with the results. The analysis of several accessions of wild tomato relatives with the three polymorphic markers allowed the establishment of the origin of the alleles found in cultivated plant material, showing that introgressions from S. lycopersicum, S. pimpinellifolium and S. habrochaites will not interfere with the results of this marker which tags Ty-1. Furthermore this analysis enabled the location of CT21, the RFLP marker from which JB-1 was designed.

A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

“…Inoculations were performed under confined conditions in an insect-proof growth chamber (see growth conditions above) appropriate for exotic virus containment. In these inoculation studies, we included the commercial tomato hybrid cultivar Anastasia (Seminis Vegetable Seeds Iberica, Almería, Spain) with TYLCD resistance based on the Ty-1 resistance gene [34]. Plants inoculated with A. tumefaciens carrying the empty vector were used as the mock-inoculated control.…”

ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis)

“…Molecular markers have emerged as a fascinating technology for evaluating genetic diversity (Parsons et al 1997;Vijayan et al 2004;Kremer et al 2005), molecular phylogenetics (Droogenbroeck et al 2004), genetic fidelity (Ray et al 2006;Joshi and Dhawan 2007) and disease resistance (Morgan et al 1998;Farinho et al 2004;Blaszezyk et al 2005;Perez de Castro et al 2007). In addition, molecular marker techniques can also offer reliable tools for the early determination of sex in plants before they enter the reproductive stage.…”

Bemisia: Bionomics and Management of a Global Pest

“…Search for resistance in Solanum lycopersicum germplasm failed, so wild relatives (Solanum section Lycopersicon) have been screened intensively (Lapidot and Friedmann 2002;Picó et al 1996). This resistance results in a dramatic reduction in virus accumulation Zamir et al 1994) and is widely used in commercial hybrids (de Castro et al 2007). Variable levels of resistance have been found in wild Solanum species used for introgression of resistance into cultivated tomato (Friedman et al 1998;Rom et al 1993;).…”