ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis)

Sharma, Kuldeep; Agrawal, Veena; Gupta, Sarika; Kumar, Ravindra; Prasad, Manoj

doi:10.1007/s11816-008-0070-7

Cited by 57 publications

(46 citation statements)

References 33 publications

(39 reference statements)

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“…Thus, this PCR-based technique is more reproducible than RAPD as it can be performed with higher stringency (high annealing temperature). ISSR markers have been used widely for genetic diversity analyses of black gram, chick pea and jojoba plants (Souframanien and Gopalakrishna 2004;Rao et al 2007;Sharma et al 2008) and both types of marker have been useful for identifying relationships at the cultivar and species level (Karuppanapandian et al 2006a(Karuppanapandian et al ,b, 2007Rao et al 2007;Sharma et al 2008). Therefore, RAPD and ISSR techniques are widely applicable because they are rapid, inexpensive and simple to perform.…”

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confidence: 99%

Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Karuppanapandian

Wang²,

Karuppudurai

et al. 2010

Can. J. Plant Sci.

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, W. 2010. Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) varieties. Can. J. Plant Sci. 90: 443Á452. The DNA fingerprinting methodologies, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to estimate genetic diversity and relationships among 20 black gram (Vigna mungo L. Hepper) varieties. Thirty selected RAPD primers amplified 255 bands, 168 of which were polymorphic (66.5%). On average, these primers produced 8.5 bands, 5.6 of which were polymorphic. Polymorphic band number varied from 2 (A-05) to 10 (OPA-02), with sizes ranging from 100 to 2550 bp. Twenty-four selected ISSR primers produced 238 amplified products, 184 of which were polymorphic (77.8%). On average, these primers generated 9.8 bands, with 7.7 polymorphic bands ranging in number from 4 (ISSR-13) to 11 (ISSR-03), and size from 100Á2650 bp. Genetic relationships were estimated using similarity coefficient (Jaccard's) values between different accession pairs; these varied from 30.7 to 85.0 for RAPD, and from 37.2 to 88.4 with ISSR. UPGMA analysis indicated that the varieties ranged in similarity from 0.50 to 1.00 (mean of 0.75) for RAPD, and from 0.47 to 1.00 (mean of 0.76) with ISSR. Cluster analysis of RAPD and ISSR results identified three clusters with significant bootstrap values, which revealed greater homology between the varieties. Principal coordinates analysis also supported this conclusion. Among the black gram varieties, WBU-108 and RBU-38 were highly divergent, whereas LBG-648 and LBG-623 were genetically similar. The markers generated by RAPD and ISSR assays can provide practical information for the management of genetic resources and these results will also provide useful information for the molecular classification and breeding of new black gram varieties.

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confidence: 99%

Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Karuppanapandian

Wang²,

Karuppudurai

et al. 2010

Can. J. Plant Sci.

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“…Further researches developed male-specific 525 and 325 bp AFLP markers utilizing EcoRI-GC/MseI-GCG and EcoRI-TAC/MseI-GCG primer sets, respectively, and a femalespecific 270 bp AFLP marker utilizing EcoRI-TAC/MseI-GCG primer set (Agarwal et al 2011). Sharma et al (2008) produced a male-specific ISSR having sequence (AG) 8 T producing 1200 bp fragment in S. chinensis (Link) Schneider, whereas the same ISSR primer amplified of around 1.1 kb in male jojoba plants by Jangra et al (2014) and subsequently converted it into an SCAR marker (GenBank accession number HQ166029.1) producing 1000 bp fragment. Heikrujam et al (2014aHeikrujam et al ( , 2014b) successfully converted two male-specific ISSR markers viz.…”

Section: Jojoba [Simmondsia Chinensis (Link) Schneider]mentioning

confidence: 99%

Sex-oriented research on dioecious crops of Indian subcontinent: an updated review

2017

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A number of dioecious species are grown across India and some of those plants play a crucial role in the agrobased economy of the country. The diagnosis of sex is very difficult in the dioecious plant prior flowering wherein sex identification at the seedling stage is of great importance to breeders as well as farmers for crop improvement or production purpose. A comprehensive approach of sex determination comprising morphological, biochemical, cytological and molecular attributes is a must required for gender differentiation in dioecious plant species. In the present review, we highlighted the economical, medicinal as well as industrial importance of most of the dioecious species extensively grown in Indian subcontinent. In addition to that, the cytogenetic, genetic as well as molecular information in connection to their sex determination were critically discussed in this review.

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“…According to Falcão et al (2004), when ISSR molecular markers were used in different organisms, significant differences can be observed in the amplification, which can be used to detect the polymorphism among the organisms studied and assess the differences among these organisms. Sharma et al (2008) identified gender differences of the species Simmondsia chinensis (jojoba) before flowering by using ISSR markers. This identification was valuable because researchers did not have any other means to detect this difference.…”

Section: Genetic Variabilitymentioning

confidence: 99%

Genetic variability and resistance of cultivars of cowpea [Vigna unguiculata (L.) Walp] to cowpea weevil (Callosobruchus maculatus Fabr.)

Nova¹,

Leite²,

Houllou³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The cowpea weevil (Callosobruchus maculatus Fabr.) is the most destructive pest of the cowpea bean; it reduces seed quality. To control this pest, resistance testing combined with genetic analysis using molecular markers has been widely applied in research. Among the markers that show reliable results, the inter-simple sequence repeats (ISSRs) (microsatellites) are noteworthy. This study was performed to evaluate the resistance of 27 cultivars of cowpea bean to cowpea weevil. We tested the resistance related to the genetic variability of these cultivars using ISSR markers. To analyze the resistance of cultivars to weevil, a completely randomized test design with 4 replicates and 27 treatments was adopted. Five pairs of the insect were placed in 30 grains per replicate. Analysis of variance showed that the number of eggs and emerged insects were significantly different in the treatments, and the means were compared by statistical tests. The analysis of the large genetic variability in all cultivars resulted in the formation of different groups. The test of resistance showed that the cultivar Inhuma was the most sensitive to both number of eggs and number of emerged adults, while the TE96-290-12-G and MNC99-537-F4 (BRS Tumucumaque) cultivars were the least sensitive to the number of eggs and the number of emerged insects, respectively.

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ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis)

Cited by 57 publications

References 33 publications

Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Sex-oriented research on dioecious crops of Indian subcontinent: an updated review

Genetic variability and resistance of cultivars of cowpea [Vigna unguiculata (L.) Walp] to cowpea weevil (Callosobruchus maculatus Fabr.)

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