First Report of Tomato Yellow Leaf Curl Virus‐Israel Species Infecting Tomato, Pepper and Bean in Tunisia

Abstract

Tomato yellow leaf curl virus disease (TYLCVD) has been observed in Tunisia for more than 20 years. Until year 2004, only the Tomato yellow leaf curl Sardinia virus-Sicily (TYLCSV-[Sic]) was detected in tomato, pepper and bean crops. In the Sahel region, some tomato samples showing severe TYLCVD symptoms were collected from greenhouses in 2004 and 2005. Typing of these isolates revealed for the first time the presence of the TYLCV Israel in Tunisia. This result was confirmed by using several sets of specific primers and by sequencing. This species has also been detected on pepper and bean collected from fields in the same region. The sequencing of a tomato and a bean isolate showed that they both share more than 97% of sequence identity with the TYLCV from Dominican Republic (AF024715). The TYLCV has been found in single and mixed infection with the TYLCSV-[Sic].www.blackwell-synergy.com

Evaluation of tomato entries with different combinations of resistance genes to tomato yellow leaf curl disease in Tunisia

“…In our study, Ty-2 alone did not offer enough protection against the Tunisian TYLCV which suggests that TYLCSV might be predominant in Tunisia; this is not surprising since TYLCSV is widespread in Mediterranean countries particularly in Italy. Accordingly, TYLCSV occurrence in Tunisia was reported several years before TYLCV-Is (Fekih-Hassen et al 2003 andGharsallah-Chouchane et al 2007).…”

“…Begomoviruses are highly diverse and endemic in the Mediterranean region, the Middle East, sub-Saharan Africa, South and South-East Asia and the Americas (Moriones and Navas-Castillo 2000, Ueda et al 2004, Brown and Idris 2006, Rojas et al 2007. Two TYLCV variants, that is tomato yellow leaf curl Sardinia virus (TYLCSV) (Fekih-Hassen et al 2003) and tomato yellow leaf curl virus-Israel (TYLCV-Is) (Gharsallah-Chouchane et al 2007), as well as recombinant variants, have been detected in Tunisia, and a molecular tool (multiplex PCR) has been developed for simultaneous detection of both variants . TYLCD symptoms appear several weeks after infection and include severe stunting if plants are infected at an early stage, a marked reduction in leaf size, upward cupping and chlorosis of the leaf margins, mottling, and flower abortion leading to up to 100% crop losses when it occurs in the nursery or early after planting Antignus 1994, Moriones andNavas-Castillo 2000).…”

“…) and tomato yellow leaf curl virus‐Israel (TYLCV‐Is) (Gharsallah‐Chouchane et al. ), as well as recombinant variants, have been detected in Tunisia, and a molecular tool (multiplex PCR) has been developed for simultaneous detection of both variants (Mnari‐Hattab et al. ).…”

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

“…Tomato yellow leaf curl virus (TYLCV) is one of the most devastating plant viruses of tomato whereas in other crops such as cucurbits and peppers is asymptomatic [185]. The virus has been reported on pepper crops in some areas of the Mediterranean basin [186,187].…”

Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam

“…Isolates were selected based on crop, collection location and sequence of the 1.5 kb DNA‐A PCR product for full‐length sequencing. Based on the alignment of the 1.5 kb PCR products and nucleotide sequences obtained from GenBank (Table S1; Alabi et al , ; Barnabas et al , ; Blair et al , ; Durham et al , , Fernandes et al , ; Gharsallah Chouchane et al , ; Gilbertson et al , ; Hameed & Robinson, ; Morinaga et al , ; Pant et al , ; Rouhibakhsh et al , ; Singh et al , ; Usharani et al , ; Yadav et al , ; Zaim et al , ), abutting primer pairs were designed to amplify the full‐length DNA‐As and DNA‐Bs of MYMIV and MYMV isolates from Indonesia and Vietnam, respectively (Table S2). Full‐length viral DNA‐A and DNA‐B were amplified from each selected sample by PCR as previously described (Tsai et al , 2011 b ).…”