Complete Nucleotide Sequence and Construction of an Infectious Clone of a Tunisian Isolate of Tomato yellow leaf curl Sardinia virus

Abstract

The full-length genome of a Tunisian isolate of Tomato yellow leaf curl Sardinia virus (TYLCSV) was engineered and submitted to sequence analysis. The Tunisian isolate has 99% sequence identity with TYL-CSV-Sicilian (Sic), results thus providing further evidence for the inclusion of this isolate in the TYL-CSV-Sic group. A 1.7-mer construct of the virus was obtained and efficiently agroinfiltrated into tomato and tobacco plants to induce symptoms indistinguishable from those of natural infection.www.blackwell-synergy.com

Molecular identification and the complete nucleotide sequence of TYLCV isolate from Shanghai of China

“…The primers were CoPR, The PCR amplifications were done in 50 ll reaction mixtures containing 4 ll of each set of primers (100 lL), 3 ll MgCl 2 (25 mmol/l), 1 ll dNTPs (10 mmol/l), 5 ll 109PCR buffer, and 0.4 ll Taq polymerase (5 U/ll) (Shanghai Sangon Biological Engineering Technology & Services Co., Ltd.). The PCR cycling parameters were as follows: 94°C for 4 min, 35 cycles of amplification at 94°C for 1 min, 50°C for 45 s, and 72°C for 45 s, followed by a final elongation step at 72°C for 10 min [12]. The amplified PCR products (15 ll volume) were separated on a 1.5% agarose gel and sequenced.…”

Επιδημιολογία και μοριακός χαρακτηρισμός των ιών του γένους begomovirus που προσβάλλουν την τομάτα στην Κύπρο και την Ελλάδα

“…O TYLCSV ανιχνεφτθκε ςε περιοριςμζνο αρικμό δειγμάτων ςτθν Κριτθ, τθ Λακωνία και τθν Αργολίδα το 2007. Θ ςφγκριςθ του γονιδιϊματοσ κατζδειξε ότι οι απομονϊςεισ αυτζσ ταυτίηονται με το ςτζλεχοσ TYLCSV-SIC που περιγράφθκε ςτθ Σικελία τθσ Λταλίασ (Kheyr-Pour et al, 1991) και το οποίο ζχει διαδοκεί ςε πολλζσ γεωγραφικζσ περιοχζσ του πλανιτθ (Chouchane et al, 2006;Tahiri et al, 2006). Ωςτόςο, ςφμφωνα με τα αποτελζςματα που λιφκθκαν μζςα από τισ ςυνεχείσ επιςκοπιςεισ, δεν υπιρξε διάδοςθ του ιοφ ςε άλλεσ περιοχζσ.…”