O Manual de métodos de análise microbiológica de alimentos e água é um manual de laboratório ilustrado contendo os métodos recomendados por órgãos internacionais (APHA, FDA, USDA, AOAC, ISO) aceitos pela Agência Nacional de Vigilância Sanitária (ANVISA). Cada capítulo traz uma revisão profunda e atualizada sobre o(s) microrganismo(s) tratado(s), incluindo posição taxonômica, mudanças na nomenclatura, características morfológicas e bioquímicas e epidemiologia. Oferece também comparações esquemáticas entre os métodos disponíveis, destacando suas diferenças e similaridades. A apresentação didática do passo a passo dos métodos em figuras esquemáticas permite uma rápida apreensão dos procedimentos, facilitando sua execução no dia a dia dos laboratórios.
Background/Aims:Cronobacter spp. have been identified as being of considerable risk to neonates. The occurrence of organisms in infant formulas is therefore of considerable interest. Methods: The occurrence of Cronobacter spp. in infant feeds (formulas and fortified cow's milk) was determined using most probable number (MPN) analysis, and from formula preparation utensils. Ninety-nine samples were analyzed, of which 42 were unopened cans of powdered infant formula (PIF), 25 reconstituted infant formulas in feeding bottles, 27 utensils used in the preparation of infant formula and 5 samples of fortified cow's milk. Presumptive Cronobacter spp. isolates were identified using the 7 allele multilocus sequence typing (MLST) scheme. Results: C. sakazakii, C. malonaticus and C. muytjensii were recovered from PIF. Although the incidence of Cronobacter in PIF was 29% (12/42), the level was low with an average of 0.54 MPN/100 g. According to MLST profiling, C. sakazakii was the most frequently isolated Cronobacter species, and C. sakazakii ST4 (associated with neonatal meningitis) was recovered from 2/42 PIF samples at 0.51 and 0.92 MPN/100 g. Conclusions:Cronobacter spp. can be isolated from PIF and therefore strict hygienic practices during PIF preparation are important to minimize neonate exposure and reduce the risk of severe infections.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.