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Frost stress is one of the major abiotic stresses causing seedling death and yield reduction in winter pea. To improve the frost tolerance of pea, field evaluation of frost tolerance was conducted on 672 diverse pea accessions at three locations in Northern China in three growing seasons from 2013 to 2016 and marker-trait association analysis of frost tolerance were performed with 267 informative SSR markers in this study. Sixteen accessions were identified as the most winter-hardy for their ability to survive in all nine field experiments with a mean survival rate of 0.57, ranging from 0.41 to 0.75. Population structure analysis revealed a structured population of two sub-populations plus some admixtures in the 672 accessions. Association analysis detected seven markers that repeatedly had associations with frost tolerance in at least two different environments with two different statistical models. One of the markers is the functional marker EST1109 on LG VI which was predicted to co-localize with a gene involved in the metabolism of glycoproteins in response to chilling stress and may provide a novel mechanism of frost tolerance in pea. These winter-hardy germplasms and frost tolerance associated markers will play a vital role in marker-assisted breeding for winter-hardy pea cultivar.

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The squat exercise was usually performed with varying feet and hip angles by different populations. The objective of this study was to compare and contrast the three-dimensional knee angles, moments, and forces during dynamic squat exercises with varying feet and hip angles. Lower extremity motions and ground reaction forces for fifteen healthy subjects (9 females and 6 males) were recorded while performing the squat with feet pointing straight ahead (neutral squat), 30º feet adduction (squeeze squat) and 30º feet abduction (outward squat). Nonparametric procedures were used to detect differences in the interested measures between the conditions. No significant difference in three-dimensional peak knee angles was observed for three squat exercises (p>0.05), however, the overall tendency of knee rotations was affected by varying feet and hip positions. During the whole cycle, the outward squat mainly displayed adduction moments, while the neutral and squeeze squat demonstrated abduction moments. Peak abduction moments were significantly affected by feet positions (p<0.05). Moreover, the tibiofemoral and patellofemoral joint forces progressively increased as knee flexed and decreased as knee extended, yet peak forces were not affected by varying feet positions (p>0.05). In conclusion, a neutral position is recommended to perform the squat exercise, while the squeeze squat and outward squat might contribute to the occurrence of joint pathologies.

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