Developing stripe rust resistant wheat (Triticum aestivum L.) lines with gene pyramiding strategy and marker-assisted selection

Liu, Rong; Lü, Jing; Zhou, Min; Zheng, Shigang; Liu, Zehou; Zhang, Chihong; Du, Mei; Wang, Minxiu; Li, Yunfang; Wu, Yu; Zhang, Lei

doi:10.1007/s10722-019-00868-5

Cited by 56 publications

(32 citation statements)

References 37 publications

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“…These mutants can clarify the role of the TF under consideration [150]. Marker assisted breeding (MAB) has a wide variety of applications in stacking of multiple genes in crop plants for various purposes and had been widely used in studying wheat rust [154]. All we need to find out for marker-assisted breeding is the tight linkage of a molecular marker (with TF as our interest).…”

Section: Crop Improvement Techniques and Tfsmentioning

confidence: 99%

“…Another area of improvement is development of functional marker systems, i.e., SSR markers, SNPs, or dCAPS for MAB of different TFs. Only a few examples exist in literature for development of functional marker systems for characterization of TFs [154][155][156]. Functional markers will help identification of TFs in successive generations for marker-assisted crop improvement.…”

Section: Future Prospects Challenges and Opportunitiesmentioning

confidence: 99%

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Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

Shahzad¹,

Jamil²,

Ahmad³

et al. 2020

Preprint

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Crop plants should be resilient to climatic factors in order to feed ever-increasing populations. Plants have developed stress-responsive mechanisms by changing their metabolic pathways and switching the stress-responsive genes. The discovery of plant transcriptional factors (TFs) as key regulators of different biotic and abiotic stresses have opened up new horizons for plant scientists. TFs perceive the signal and switch certain stress-responsive genes on and off by binding to different cis-regulatory elements. The above 50 species of plant TFs have been reported in nature. DREB, bZIP, MYB, NAC, Zinc-finger, HSF, Dof, WRKY, and NF-Y are important with respect to biotic and abiotic stresses whereas the role of many TFs is yet to explore. In this review, we summarize the role of different stress-responsive TFs with respect to biotic and abiotic stresses. Further, challenges and future opportunities linked with TFs for developing climate-resilient crops are also elaborated.

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Section: Crop Improvement Techniques and Tfsmentioning

confidence: 99%

Section: Future Prospects Challenges and Opportunitiesmentioning

confidence: 99%

Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

Shahzad¹,

Jamil²,

Ahmad³

et al. 2020

Preprint

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“…CY12 is developed by our research group and highly susceptible to currently predominant stripe rust. L58 is a pyramiding line (Yr10, Yr15, Yr30 and Yr65) with CY12 as background which shows high resistance to new Pst-race CYR34 in our previous research [39]. Artificial inoculation at Seedling stage was conducted under a controlled greenhouse condition in Gansu Academy of Agricultural Sciences (GAAS).…”

Section: Plant Materials and Fungal Treatmentmentioning

confidence: 99%

“…Different responses to the Pst infection were observed in CY12 (susceptible) and L58 (resistance) at seedling stage and adult-plant stage ( Figure 1). L58 is offspring of CY12, and in previous study we use molecular assistant selection to detected four Yr genes (Yr10, Yr15, Yr30 and Yr65) in L58 which located on B chromosome [39].…”

Section: Phenotype Identification Of Two Wheat After Pst Infectionmentioning

confidence: 99%

Transcriptomic analysis reveals the molecular mechanisms of wheat seedling resistance to Puccinia striiformis f. sp. tritici

Liu¹,

Lu²,

Du³

et al. 2020

Preprint

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Background: Stripe rust or yellow rust (Yr), caused by Puccinia striiformis f. sp. Tritici (Pst), is one of the most globally devastating fungal disease that significantly reduces yield and quality in wheat (Triticum aestivum). Although some Yr genes have been successfully used in wheat breeding and a little number of them have been cloned, large of the regulating networks and the molecular mechanisms of Pst resistance remains unknown. In this study, a pair of Yr-gene pyramiding line L58 and its background parent cv. Chuanyu12 (CY12) were used to study the transcriptome profiles after inoculated with Pst physiological race CYR34. Results: The results revealed that the different expression genes (DEGs) were significantly enriched in phenylpropanoid biosynthesis, phenylalanine metabolism, plant-pathogen interaction and MAPK signaling pathways after Pst-CYR34 inoculation. Compared with CY12, L58 showed greater up-regulated DEGs in those pathways by Pst infection at 24hpi. However, these DEGs became lower expression in L58 and opposite expression in CY12 at 7dpi. Besides, the activities of enzymes (PAL, POD) and products of phenylpropanoid pathway (lignin content) were significantly increased in both CY12 and L58, and the increase was greater and faster in the resistant line L58. Some candidate genes and transcription factors (TFs) associated with Pst resistance were identified, including LRR receptor-like serine/threonine protein kinase, disease resistance protein, MYB, NAC and WRKY transcription factors involved in the fine-tuning of Pst infection responses. Conclusions: Our results give insights into the regulating networks of Pst resistance and pave the way for durable resistant breeding in bread wheat.

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“…Many studies have been done on stripe rust resistance using GWAS and identi ed many QTLs controlling the resistance distributing across the whole genome [29][30][31]. In addition, many simple sequence repeats (SSR), sequence-Tagged site (STS), sequence-characterized ampli ed region (SCAR), diversity arrays technology (DArT), and Kompetitive allele-speci c PCR (KASP) markers have been reported as good markers for MAS for stripe rust resistance genes in wheat [32][33][34][35]. Furthermore, Maccaferri et al, (2015) reported association mapping of the resistance to the American race of P. striiformis using a world-wide collection of spring wheat genotyped by DArT markers.…”

Section: Introductionmentioning

confidence: 99%

Genomic regions associated with stripe rust resistance against the Egyptian race revealed by genome-wide association study

Mohamed

Mourad

2020

Preprint

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Background: Wheat stripe rust (caused by Puccinia striiformis f. sp. Tritici), is a major disease that causes huge yield damage. New pathogen races appeared in the last few years and caused a broke down in the resistant genotypes. In Egypt, some of the resistant genotypes began to be susceptible to stripe rust in recent years. This situation increases the need to produce new genotypes with durable resistance. Besides, looking for a new resistant source from the available wheat genotypes all over the world help in enhancing the breeding programs. Results: In the recent study, a set of 103-spring wheat genotypes from different fourteen countries were evaluated to their field resistant to stripe rust for two years. These genotypes included 17 Egyptian genotypes from the old and new cultivars. The 103-spring wheat genotypes were reported to be well adapted to the Egyptian environmental conditions. Out of the tested genotypes, eight genotypes from four different countries were found to be resistant in both years. Genotyping was carried out using genotyping-by-sequencing and a set of 26,703 SNPs were used in the genome-wide association study. Five SNP markers, located on chromosomes 2A and 4A, were found to be significantly associated with the resistance in both years. Three gene models associated with disease resistance and underlying these significant SNPs were identified. One immune Iranian genotype, with the highest number of different alleles from the most resistant Egyptian genotypes, was detected. Conclusion: the high variation among the tested genotypes in their resistance to the Egyptian stripe rust race confirming the possible improvement of stripe rust resistance in the Egyptian wheat genotypes. The identified five SNP markers are stable and could be used in marker-assisted selection after validation in different genetic backgrounds. Crossing between the immune Iranian genotype and the Egyptian genotypes will improve stripe rust resistance in Egypt.

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Developing stripe rust resistant wheat (Triticum aestivum L.) lines with gene pyramiding strategy and marker-assisted selection

Cited by 56 publications

References 37 publications

Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

Transcriptomic analysis reveals the molecular mechanisms of wheat seedling resistance to Puccinia striiformis f. sp. tritici

Genomic regions associated with stripe rust resistance against the Egyptian race revealed by genome-wide association study

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