Leptospirosis is still a health problem in the word. Leptospirosis can be transmitted to humans trough contact with environment infected with pathogenic Leptospira. Efforts to control pathogenic Leptospira in the environment can be done one of them by disinfecting. Effective desinfectants for the control of pathogenic Leptospira include the active ingredients Calcium hypochlorite 60% and Sodium hypochlorite 5,25%. This study aims to determine the effectiveness of Calcium hypochlorite 60% and Sodium hypochlorite 5,25% against the life power of pathogenic Leptospira. This research is a laboratory study with pure experimental design. Serovar of pathogenic Leptospira used is Leptospira icterohaemorrhagiae. Leptospira icterohaemorrhagiae desnsity used as much as 5.7 x 106 Leptospira/ml. The concentrations of desinfectans and bacteria are: 5 ppm, 50 ppm, 500 ppm, 1000 ppm, 2000 ppm, 5000 ppm, 10.000 ppm, and 25.000 ppm. Observations are made every ten minutes and twenty minutes with three repeats. The results showed that Leptospira icterohaemorrhagiae could not survive when tested on 60% calcium hypochlorite and 5.25% sodium hypochlorite, respectively, at a concentration of 2000 ppm and a concentration of 1000 ppm. Statistical results showed there was a significant difference in the concentration of each disinfectant active ingredient. Thus, a disinfectans of chlorine with the active ingredient Calcium hypochlorite 60% and Sodium hypochlorite 5,25% effectively affects the viability of the Leptospira icterohaemorrhagiae. Keywords : Sodium Hypochlorite, Calcium Hypochlorite, Leptospira, Disinfectant Abstrak Leptospirosis masih menjadi masalah kesehatan di dunia. Leptospirosis dapat menular ke manusia lewat kontak dengan lingkungan yang mengandung Leptospira patogenik. Upaya pengendalian Leptospira patogenik di lingkungan dapat dilakukan salah satunya dengan pemberian disinfektan. Disinfektan yang efektif untuk pengendalian Leptospira patogenik diantaranya adalah bahan aktif kalsium hipoklorit 60% dan sodium hipoklorit 5,25%. Penelitian ini bertujuan mengetahui efektivitas bahan aktif kalsium hipoklorit 60% dan sodium hipoklorit 5,25% terhadap daya hidup Leptospira patogenik. Penelitian ini merupakan penelitian skala laboratorium dengan rancangan eksperimental murni. Serovar Leptospira patogenik yang digunakan adalah Leptospira icterohaemorrhagiae. Kepadatan Leptospira icterohaemorrhagiae yang digunakan sebanyak 5,7 x 106 Leptospira/ml. Konsentrasi campuran disinfektan dan bakteri yaitu: 5 ppm, 50 ppm, 500 ppm, 1000 ppm, 2000 ppm, 5000 ppm, 10.000 ppm, dan 25.000 ppm. Pengamatan dilakukan tiap 10 menit dan 20 menit dengan tiga kali ulangan. Hasil penelitian menunjukkan Leptospira icterohaemorrhagiae tidak dapat hidup saat diujikan ke kalsium hipoklorit 60% dan sodium hipoklorit 5,25% berturut-turut pada konsentrasi 2000 ppm dan konsentrasi 1000 ppm. Hasil statistik menunjukkan ada perbedaan signifikan dari besaran konsentrasi masing-masing bahan aktif disinfektan. Dengan demikian, disinfektan klorin dengan bahan aktif calcium hypochlorite 60% dan sodium hypochlorite 5,25% efektif memengaruhi daya hidup Leptospira icterohaemorrhagiae. Kata kunci: Sodium hipoklorit, Kalsium hipoklorit, Leptospira, Disinfektan
Abstract Malaria is a vector-borne disease that is still a health problem in Indonesia. Control of vector mosquito larvae using Bacillus thuringiensis (Bt H-14) is an alternative biolarvicide. B2P2VRP has developed Salatiga Bt H-14 isolate in powder preparation which need to be tested for effectiveness stability. This study aims to determine the effectiveness stability of Salatiga powder Bt H-14 isolate against larvae of Anopheles spp. Preparation of Salatiga powder Bt H-14 isolate and its bioassay test against larvae of Anopheles aconitus were carried out at laboratory of the B2P2VRP. The stability test was carried out at the laboratory of UII Yogyakarta. The stability effectiveness test was carried out in the field in Purworejo using Anopheles spp. larvae taken in the field. The bioassay test (LC90 ) at initial and after stored at 54 o C ± 2 o C for two weeks were 6.485 ppm and 13.009 ppm, respectively. The study showed the effectiveness of stability decreased on days 2 and 3 (<70%) at dose of 26,018 ppm because of rain. Statistical test showed a difference in the effectiveness of reducing larvae mortality at days 2 and 3. The study confirmed the reduction of effectiveness of Salatiga powder Bt H-14 Isolate after stability test. Keywords: Anopheles larvae, Bacillus thuringiensis, effectiveness, Purworejo, stability Abstrak Malaria merupakan salah satu penyakit tular vektor yang masih menjadi masalah kesehatan di Indonesia. Pengendalian jentik nyamuk vektor menggunakan Bacillus thuringiensis (Bt H-14) merupakan salah satu biolarvisida alternatif. B2P2VRP telah mengembangkan Bt H-14 isolat Salatiga sediaan bubuk maka perlu diuji stabilitas. Penelitian ini bertujuan menentukan efektivitas stabilitas Bt H-14 isolat Salatiga sediaan bubuk terhadap jentik Anopheles spp di lapangan. Pembuatan Bt H-14 Isolat Salatiga sediaan bubuk dan uji bioassay terhadap jentik Anopheles aconitus dilakukan di laboratorium B2P2VRP. Uji stabilitas dilakukan di laboratorium UII Yogyakarta. Uji efektivitas stabilitas dilakukan di Purworejo menggunakan jentik Anopheles spp. yang diambil di lapangan. Hasil uji bioassay (LC90 ) sebelum dan setelah penyimpanan suhu 54 o C ± 2 o C selama 2 minggu adalah 6,485 ppm dan 13,009 ppm. Hasil menunjukkan efektivitas stabilitas menurun pada hari ke 2 dan 3 (<70%) pada dosis 26,018 ppm adanya pengaruh hujan. Uji statistik menunjukkan adanya perbedaan efektivitas penurunan kematian jentik saat hari ke 2 dan 3. Hasil penelitian menunjukkan adanya penurunan efektivitas dari Bt H-14 Isolat Salatiga sediaan bubuk setelah dilakukan uji stabilitas. Kata kunci : Anopheles larvae, Bacillus thuringiensis, efektivitas, Purworejo, stabilitas
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