Bantul is one of leptospirosis endemic areas which has been reported the cases every year and the presence of rats is an important risk factor relating to leptospirosis incidence in Bantul. Therefore, the role of rats as reservoir was examined in three types of ecosystems: forest, non-forest, and coastal ecosystems. Rat trapping was carried out using 100 single livetraps which were distributed in 9 locations: 2 points in the forest, 5 points in the non-forest, and 2 points in the coastal ecosystem. The rats were identified and their kidneys were collected and preserved in 70% alcohol medium. Leptospira were detected in the kidney by using PCR method. A number of 196 rats were obtained during the study. Most of them were commensal rats obtained from settlements ecosystems. Rattus tanezumi was the dominant species in the three ecosystems, but the highest infection rate of leptospira pathogen was in R.norvegicus. Coastal ecosystems were contained more infected rats, where the main habitat were mangrove forests. Based on these results, rats have great potency in leptospirosis transmission in Bantul, especially in settlements and coastal areas although leptospirosis cases were reported rarely in study area. Nonetheless, awareness of transmission should be disseminated since the presence of pathogenic leptospira in rats is very high. Keywords: leptospirosis, rat, reservoir, ecosystem, Bantul Abstrak Kabupaten Bantul merupakan salah satu daerah endemis leptospirosis yang sejak lama melaporkan kasus ini setiap tahunnya dan keberadaan tikus merupakan faktor risiko penting yang mempengaruhi kejadian leptospirosis di Kabupaten Bantul. Oleh karena itu peranan tikus sebagai hewan reservoir diteliti di tiga jenis ekosistem yaitu ekosistem hutan, non-hutan, dan pesisir. Penangkapan tikus dilakukan menggunakan perangkap tunggal sebanyak 100 buah yang disebar di 9 titik lokasi, meliputi dua titik di ekosistem hutan, lima titik di ekosistem non-hutan, dan dua titik di ekosistem pesisir. Tikus tertangkap diidentifikasi kemudian organ ginjalnya dikoleksi dan diawetkan dalam medium alkohol 70%. Bakteri leptospira dideteksi pada specimen ginjal dengan metode PCR dari total 196 tikus diperoleh selama penelitian. Sebagian besar tikus diperoleh dari ekosistem dekat pemukiman dan semuanya merupakan tikus komensal. Rattus tanezumi merupakan spesies dominan di ketiga ekosistem, namun persentase infeksi leptospira patogen paling tinggi terdapat pada R. norvegicus. Ekosistem pesisir mengandung tikus terinfeksi leptospira patogenik paling tinggi, dengan habitat utama hutan mangrove. Berdasarkan hasil ini maka tikus berpotensi besar menularkan leptospirosis di Kabupaten Bantul, terutama di daerah pemukiman dan pesisir. Meskipun kasus leptospirosis di daerah penelitian sedikit sekali dilaporkan, namun kewaspadaan terhadap penularan perlu dilakukan sedini mungkin karena leptospira patogenik yang terkandung dalam tikus tergolong tinggi. Kata kunci: leptospirosis, tikus, reservoir, ekosistem, Bantul
Leptospirosis is still a health problem in the word. Leptospirosis can be transmitted to humans trough contact with environment infected with pathogenic Leptospira. Efforts to control pathogenic Leptospira in the environment can be done one of them by disinfecting. Effective desinfectants for the control of pathogenic Leptospira include the active ingredients Calcium hypochlorite 60% and Sodium hypochlorite 5,25%. This study aims to determine the effectiveness of Calcium hypochlorite 60% and Sodium hypochlorite 5,25% against the life power of pathogenic Leptospira. This research is a laboratory study with pure experimental design. Serovar of pathogenic Leptospira used is Leptospira icterohaemorrhagiae. Leptospira icterohaemorrhagiae desnsity used as much as 5.7 x 106 Leptospira/ml. The concentrations of desinfectans and bacteria are: 5 ppm, 50 ppm, 500 ppm, 1000 ppm, 2000 ppm, 5000 ppm, 10.000 ppm, and 25.000 ppm. Observations are made every ten minutes and twenty minutes with three repeats. The results showed that Leptospira icterohaemorrhagiae could not survive when tested on 60% calcium hypochlorite and 5.25% sodium hypochlorite, respectively, at a concentration of 2000 ppm and a concentration of 1000 ppm. Statistical results showed there was a significant difference in the concentration of each disinfectant active ingredient. Thus, a disinfectans of chlorine with the active ingredient Calcium hypochlorite 60% and Sodium hypochlorite 5,25% effectively affects the viability of the Leptospira icterohaemorrhagiae. Keywords : Sodium Hypochlorite, Calcium Hypochlorite, Leptospira, Disinfectant Abstrak Leptospirosis masih menjadi masalah kesehatan di dunia. Leptospirosis dapat menular ke manusia lewat kontak dengan lingkungan yang mengandung Leptospira patogenik. Upaya pengendalian Leptospira patogenik di lingkungan dapat dilakukan salah satunya dengan pemberian disinfektan. Disinfektan yang efektif untuk pengendalian Leptospira patogenik diantaranya adalah bahan aktif kalsium hipoklorit 60% dan sodium hipoklorit 5,25%. Penelitian ini bertujuan mengetahui efektivitas bahan aktif kalsium hipoklorit 60% dan sodium hipoklorit 5,25% terhadap daya hidup Leptospira patogenik. Penelitian ini merupakan penelitian skala laboratorium dengan rancangan eksperimental murni. Serovar Leptospira patogenik yang digunakan adalah Leptospira icterohaemorrhagiae. Kepadatan Leptospira icterohaemorrhagiae yang digunakan sebanyak 5,7 x 106 Leptospira/ml. Konsentrasi campuran disinfektan dan bakteri yaitu: 5 ppm, 50 ppm, 500 ppm, 1000 ppm, 2000 ppm, 5000 ppm, 10.000 ppm, dan 25.000 ppm. Pengamatan dilakukan tiap 10 menit dan 20 menit dengan tiga kali ulangan. Hasil penelitian menunjukkan Leptospira icterohaemorrhagiae tidak dapat hidup saat diujikan ke kalsium hipoklorit 60% dan sodium hipoklorit 5,25% berturut-turut pada konsentrasi 2000 ppm dan konsentrasi 1000 ppm. Hasil statistik menunjukkan ada perbedaan signifikan dari besaran konsentrasi masing-masing bahan aktif disinfektan. Dengan demikian, disinfektan klorin dengan bahan aktif calcium hypochlorite 60% dan sodium hypochlorite 5,25% efektif memengaruhi daya hidup Leptospira icterohaemorrhagiae. Kata kunci: Sodium hipoklorit, Kalsium hipoklorit, Leptospira, Disinfektan
Leptospirosis is a disease caused by pathogenic Leptospira and is still a health problem in the community. Until now, West Papua Province has not reported any cases of leptospirosis. This study aims to examine whether there is a grouping pattern of Leptospira harboring rats and identify the habitat types of positive rat Leptospira. This research was descriptive observational research using a cross-sectional approach. Research locations are in Manokwari, Fakfak, and Raja Ampat districts in West Papua Province. The catching of rats was carried out in locations of forest ecosystem settlements, near forest settlements, non-forested far from settlements, non-forests near settlements, coastal remote settlements, and beaches near settlements. Leptospira in rats was detected by examination of Polymerase Chain Reaction (PCR) and Microscopic Agglutination Test (MAT). The results showed 278 rats were caught. The number of Leptospira positive rats in three districts by PCR was 34 (12.2%) and MAT were 13 (4.7%). Leptospira positive habitat types were settlements, yards, and secondary forests. The SaTScan results were obtained by six positive Leptospira rat clusters. There were no significant results which showed that no spread of Leptospira in the clusters. The presence of Leptospira in rats should be a warning for leptospirosis transmission risk at the study site.
Leptospirosis has become an important public health problem in Demak District, Central Java, Indonesia. Early diagnosis and appropriate treatments have been carried out to prevent an increase of leptospirosis cases in this region. However, these efforts are still hampered due to the lack of knowledge of leptospirosis, especially in the primary health care level. It is necessary to take effective clinical Leptospirosis training approaches to improve primary health care workers' competency on leptospirosis surveillance. The aim of this study was to strengthen the capacity of the primary health care workers to improve their knowledge on clinical leptospirosis. The quasi-experimental study (one group pre-post test without control) was conducted among 34 participants from all subdistrict health offices in Demak District, Central Java. The training was administered for one day with assessment at the baseline and the post-training. The four training outcomes were score changes in workers' knowledge of the clinical manifestations of leptospirosis suspected cases, probable cases; the appropriate time of using Rapid diagnostic tests for diagnosing leptospirosis suspects; and the implementation of active and passive case detection. The result showed a statistically significant difference in knowledge achievement between pre-training (66.46±2.16) and post-training (80.78±1.82). The percentage of the total knowledge between the baseline and the post-training increased significantly by 22.39%. These findings are expected to give a contribution to workers' knowledge to improve the competency of public health care workers on clinical leptospirosis and recommended to be disseminated in similar settings to strengthen leptospirosis surveillance.
Leptospirosis is a zoonosis with a wide spread, both in Indonesia and globally. Leptospirosis risk factors are mainly behavioral and environmental factors. This study aims to describe the knowledge, behavior and environment of leptospirosis infected areas in Karangkandri Village, Cilacap Regency, Central Java. The research design was cross sectional with descriptive analysis. Respondents in the study were residents who lived in areas where leptospirosis cases occurred. Data collection was carried out by interview with a structured questionnaire instrument. The results showed that respondents who had knowledge of leptospirosis in the less category were 71.43%, 25.71% in the moderate category and 2.86% in the good category. The behavior of respondents in the less category was 85.71% and 14.29% was moderate, there was no good category. Environmental conditions in 14.29% for the less category and the medium category for 85.71%, there was no good category. Between knowledge and education there was a significant relationship (p <0.05), while knowledge with behavior and knowledge with the environment has an insignificant relationship (p> 0.05). Keywords: Leptospirosis, knowledge, behavior, environment Abstrak Leptospirosis merupakan zoonosis dengan penyebaran yang luas, baik di Indonesia maupun secara global. Faktor risiko terkait dengan kejadian leptospirosis terutama faktor perilaku dan lingkungan. Penelitian ini bertujuan untuk mendeskripsikan pengetahuan, perilaku dan lingkungan wilayah tertular leptospirosis di Desa Karangkandri, Kabupaten Cilacap, Jawa Tengah. Rancangan penelitian ini adalah cross sectional dengan analisis secara deskriptif. Responden penelitian adalah warga yang bertempat tinggal di wilayah terjadi kasus leptospirosis. Pengumpulan data dilakukan dengan wawancara dengan instrumen kuesioner terstruktur. Hasil penelitian menunjukkan bahwa responden yang memiliki pengetahuan tentang leptospirosis kategori kurang sebesar 71,43%, kategori sedang sebesar 25,71% dan 2,86% kategori baik. Perilaku responden kategori kurang sebesar 85,71% dan sedang sebesar 14,29%, kategori baik tidak ada. Kondisi lingkungan kategori kurang 14,29% dan kategori sedang sebesar 85,71% tidak ada kategori baik. Antara pengetahuan dengan pendidikan terdapat hubungan yang signifikan (p<0,05), sedang pengetahuan dengan perilaku dan pengetahuan dengan lingkungan terdapat hubungan yang tidak signifikan (p>0,05). Kata kunci: Pengetahuan, perilaku, lingkungan, leptospirosis.
Cultivation of Daemonorops draco is one of the conservation strategies to conserve its population in nature. Complete data and information about morphology are needed to support cultivation techniques. There has been much research on the morphology of D. draco, but the research of rattan spines is lack. Rattan spines have an important function in protecting themselves against herbivores or seed predators. Growing media is one of the factors that influence the growth of rattan spines. We studied the growth of rattan spines from several compositions of organic media in nurseries. The experiment was arranged in a completely randomized design, with five treatments and three replications. The treatments were compositions of organic media content, i.e.: 0%, 25%, 50%, 75%, and 100%. The variables observed were the number of rattan spines, spines color, and spines angle. Our results showed that the media composition of 75% organic matter tends to show higher spines growth response even though statistically not significantly different with 100% mineral soils. Spine’s color was light green to yellow. Spines angle was pointed downward towards the grounds.
Abstract Malaria is a vector-borne disease that is still a health problem in Indonesia. Control of vector mosquito larvae using Bacillus thuringiensis (Bt H-14) is an alternative biolarvicide. B2P2VRP has developed Salatiga Bt H-14 isolate in powder preparation which need to be tested for effectiveness stability. This study aims to determine the effectiveness stability of Salatiga powder Bt H-14 isolate against larvae of Anopheles spp. Preparation of Salatiga powder Bt H-14 isolate and its bioassay test against larvae of Anopheles aconitus were carried out at laboratory of the B2P2VRP. The stability test was carried out at the laboratory of UII Yogyakarta. The stability effectiveness test was carried out in the field in Purworejo using Anopheles spp. larvae taken in the field. The bioassay test (LC90 ) at initial and after stored at 54 o C ± 2 o C for two weeks were 6.485 ppm and 13.009 ppm, respectively. The study showed the effectiveness of stability decreased on days 2 and 3 (<70%) at dose of 26,018 ppm because of rain. Statistical test showed a difference in the effectiveness of reducing larvae mortality at days 2 and 3. The study confirmed the reduction of effectiveness of Salatiga powder Bt H-14 Isolate after stability test. Keywords: Anopheles larvae, Bacillus thuringiensis, effectiveness, Purworejo, stability Abstrak Malaria merupakan salah satu penyakit tular vektor yang masih menjadi masalah kesehatan di Indonesia. Pengendalian jentik nyamuk vektor menggunakan Bacillus thuringiensis (Bt H-14) merupakan salah satu biolarvisida alternatif. B2P2VRP telah mengembangkan Bt H-14 isolat Salatiga sediaan bubuk maka perlu diuji stabilitas. Penelitian ini bertujuan menentukan efektivitas stabilitas Bt H-14 isolat Salatiga sediaan bubuk terhadap jentik Anopheles spp di lapangan. Pembuatan Bt H-14 Isolat Salatiga sediaan bubuk dan uji bioassay terhadap jentik Anopheles aconitus dilakukan di laboratorium B2P2VRP. Uji stabilitas dilakukan di laboratorium UII Yogyakarta. Uji efektivitas stabilitas dilakukan di Purworejo menggunakan jentik Anopheles spp. yang diambil di lapangan. Hasil uji bioassay (LC90 ) sebelum dan setelah penyimpanan suhu 54 o C ± 2 o C selama 2 minggu adalah 6,485 ppm dan 13,009 ppm. Hasil menunjukkan efektivitas stabilitas menurun pada hari ke 2 dan 3 (<70%) pada dosis 26,018 ppm adanya pengaruh hujan. Uji statistik menunjukkan adanya perbedaan efektivitas penurunan kematian jentik saat hari ke 2 dan 3. Hasil penelitian menunjukkan adanya penurunan efektivitas dari Bt H-14 Isolat Salatiga sediaan bubuk setelah dilakukan uji stabilitas. Kata kunci : Anopheles larvae, Bacillus thuringiensis, efektivitas, Purworejo, stabilitas
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