In Indonesia, leptospirosis often causes outbreaks. Three important sources of transmission of leptospirosis are the environment contaminated with leptospira, the presence of rats and presence of livestock as reservoirs. This study aims to determine the reservoir that plays a role in the leptospirosis transmission in increased case areas of leptospirosis in the Pagedangan Ilir Village, Kronjo Sub-district, Tangerang. Rat traping was done twice. Each trapping was done two times, in consecutive days. Livestock sampling was also conducted in the study area. Rat and livestock serum specimens were collected for leptospirosis detection using MAT, while rat kidneys were collected for PCR test. Leptospirosis detection was performed by PCR and MAT. The results of the first and second rat traping showed relative density of 17% and 20% in the houses, as well as 14% and 10% in outdoor area. Rat traped consist of two species, Rattus norvegicus and Rattus tanezumi. The prevalence of pathogenic leptospira in rat (with PCR assay) was 33% in the first trap and 26% in the second trap. The MAT test showed a positive 14% on R. norvegicus in the first trap, but negative in the second trap. While livestock did not show PCR positive, but one serological positive sample (by MAT) with low titers (1:20) was found against the serovar Robinsoni
Leptospirosis merupakan penyakit zoonosis disebabkan bakteri Leptospira patogenik dan berpotensi menyebabkan kematian pada manusia. Tikus sebagai reservoir utama Leptospira kebanyakan diteliti di kawasan perkotaan atau pedesaan di sekitar permukiman. Sebaliknya, penelitian leptospirosis pada tikus endemis jumlahnya sedikit. Penelitian ini bertujuan untuk mengidentifikasi jenis tikus endemis Sulawesi sebagai reservoir Leptospira di Kabupaten Bulukumba, Pangkep dan Luwu Timur. Penelitian ini merupakan bagian dari Rikhus Vektora tahun 2017 dengan menggunakan perangkap hidup untuk menangkap tikus di enam ekosistem berbeda pada tiap kabupaten. Seluruh tikus tertangkap diidentifikasi secara morfologis yang selanjutnya diuji Microscopic Agglutination Test (MAT) dan Polymerase Chain Reaction (PCR). Hasilnya diperoleh berbagai tikus endemis seperti Rattus marmosurus, Rattus hoffmanni, Bunomys chrysocomus, Bunomys andrewsi dan Bunomys coelestis terinfeksi leptospirosis. Hasil penelitian menunjukkan catatan baru beberapa jenis tikus endemis Sulawesi terinfeksi leptospirosis. Kondisi ini perlu diwaspadai karena terdapat potensi penularan leptospirosis di habitat hutan dari tikus endemis tersebut.
Beside as seed dispersal and high valued plant pollinator, frugivorous bat (Pteripodidae) also has a role as reservoir of Leptospira. This study was aimed to survey the the prevalence of Leptospira among Pteripodidae in three regencies of Central Java Province namely Purworejo, Pati and Pekalongan, and their potential as reservoir of Leptospira. This study used four mist nets and a harp trap to collect bat at each site of each regency which has six sites. The caught bats were identified morphologically and followed by serological examination by Microscopic Aglutinasion Test (MAT). A total of 278 Pteripodidae was examined by MAT consisting of five genera with eight species. The result of MAT for Leptospira showed that there are no examined bats were positif (0%). It indicated there is no sufficient evidence that Pteripodidae as potential reservoir of Leptospira in Central Java.
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