Monitoring and analysis of blood pack faults has resulted in the identification and rectification of specific problems associated with blood pack manufacture and use. Close collaboration with manufacturers has enabled effective remedial action. Wider collation of data would provide an early warning of potential areas of concern.
The changes in electrode potential recorded by a platinum foil electrode, and in pH, during the incubation of chstridium butyricum spores in a medium continuoudy sparged with a sterile gas mixture have been correlated with the biological events occurring simultaneously. The marked fall in Eh which occurs during incubation is recorded by this type of electrode w occurring during the period of rapid cell replication; this observation may be an artefact associated with foil electrodes and the authors feel that results obtained with such electrodes should be interpreted with caution. The stimulating effect of GO, and the increasingly inhibitory effect on germination of 0,, as the concentration of the latter in the gas mixture is increased, have been noted. The inhibitory effect is thought t o be due to the Oa itself and not t o the high En produced in the medium by the 0,.
Spores of Bacillus subtilis were filtered on membrane flters and then exposed to solutions of cetrimide. The spores, and the membranes themselves, even after being washed retained inhibitory concentrations of cetrimide. This might present a problem in counting bacterial survivors of cetrimide action by a membrane technique. The inhibitory effect of the low cetrimide concentrations retained by the membranes and the spores can be eliminated by washing with a Lubrol-lecithin solution.H
Methods have been investigated for isolating and enumerating, by a membrane filtration technique, E. coli inoculated into white soft paraffin. Of three solvents used to prepare the ointment base for filtration, the best recovery (54 %) was obtained with light liquid paraffin. Superior recovery levels (ca 75%) were obtained by homogenizing the ointment base with a Tween 80/peptone water mixture. Recovery levels were greatly affected by the weight of ointment base taken but the Tween 80/peptone water system permitted the estimation of the contamination in up to 0.25 g of white soft paraffin.
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