1973
DOI: 10.1111/j.2042-7158.1973.tb09157.x
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The recovery of bacteria from white soft paraffin

Abstract: Methods have been investigated for isolating and enumerating, by a membrane filtration technique, E. coli inoculated into white soft paraffin. Of three solvents used to prepare the ointment base for filtration, the best recovery (54 %) was obtained with light liquid paraffin. Superior recovery levels (ca 75%) were obtained by homogenizing the ointment base with a Tween 80/peptone water mixture. Recovery levels were greatly affected by the weight of ointment base taken but the Tween 80/peptone water system perm… Show more

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Cited by 12 publications
(4 citation statements)
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“…Meetings are facilitated by a nonprofessional volunteer who has undergone an online training course providing the required knowledge of the program’s foundations (Horvath & Velten, 2000). As of 2012, 698 SMART meetings operated in the United States, Canada, Australia, and Great Britain (Allwood & White, 2013).…”
Section: Smart Recovery: Principles Philosophy and Mechanisms For Cmentioning
confidence: 99%
“…Meetings are facilitated by a nonprofessional volunteer who has undergone an online training course providing the required knowledge of the program’s foundations (Horvath & Velten, 2000). As of 2012, 698 SMART meetings operated in the United States, Canada, Australia, and Great Britain (Allwood & White, 2013).…”
Section: Smart Recovery: Principles Philosophy and Mechanisms For Cmentioning
confidence: 99%
“…Mixing cream with isopropyl myristate gave bacterial recoveries of 30-73%. Allwood & Hambleton (1973) and Walsh & Allwood (1973) reported that improved recovery could be obtained by combining membrane filtration with a medium containing 1 % peptone and 1 % Tween-80. This raised recovery rates for Escherichia coli to 74-82 % compared with 5-11 % with isopropyl myristate.…”
Section: Discussionmentioning
confidence: 99%
“…The difficulty of quantitative bacterial recovery from ointments is well documented (Allwood & Hambleton, 1973;Sokolski & Chidester, 1964 . Although preparations containing paraffin have a low water miscibility and represent particular problems, our cream was water soluble and so should have been easier to sample.…”
Section: Recovery Of Test Bacteriamentioning
confidence: 99%
“…The publication of the United States Pharmacopoeia officialized this method in 1970 [2]. Since then, several authors have reported the sensitivity of microorganisms to isopropyl myristate [3][4][5][6], particularly Pseudomonas aeruginosa, the bacterium most sensitive to this fatty acid ester [7]. Thus, the use of isopropyl myristate in the sterility test of ointments can produce false-negative results and cause health * Corresponding author.…”
Section: Introductionmentioning
confidence: 99%