Spores of Bacillus subtilis were filtered on membrane flters and then exposed to solutions of cetrimide. The spores, and the membranes themselves, even after being washed retained inhibitory concentrations of cetrimide. This might present a problem in counting bacterial survivors of cetrimide action by a membrane technique. The inhibitory effect of the low cetrimide concentrations retained by the membranes and the spores can be eliminated by washing with a Lubrol-lecithin solution.H
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