Heat and Gamma-Radiation Resistance of <i>Bacillus Megaterium</i> Spores

Tallentire, A.; Chiori, C. O.

doi:10.1111/j.2042-7158.1963.tb11203.x

Cited by 3 publications

(3 citation statements)

References 2 publications

(2 reference statements)

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“…5) were due to the loss of cations. Since other investigators have indicated that various spore ions have no apparent role in spore resistance to -y-radiation (11) or to ultraviolet irradiation (10), it was therefore not surprising that the mild treatment with thioglycolate had no effect on this facet of spore behavior.…”

Section: Methodsmentioning

confidence: 99%

Changes in Spores of Bacillus megaterium Treated with Thioglycolate at a Low p H and Restoration of Germinability and Heat Resistance by Cations

Rowley

Levinson

1967

J Bacteriol

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Spores of Bacillus megaterium QM B1551 treated with thioglycolate (0.4 M, pH 2.6) at 50 C for 30 min remained refractile, but they became stainable, lysozymesensitive, and nonviable, and they lost dipicolinic acid (DPA). The loss of DPA and of viability were functions of the time and temperature of exposure to thioglycolate. Spores treated with thioglycolate at a lower temperature and for a shorter time (30 C, 5 min) retained DPA, viability, and nonstainability. Although these spores also retained their resistance to y radiation and to lysozyme, they lost thermoresistance. Their percentage of germination over a 2-hr period in glucose was markedly reduced. Germinability and heat resistance were restored by exogenous cations, suggesting that the thioglycolate treatment (30 C, 5 min) resulted in the loss of spore ions essential for normal germination in glucose and for heat resistance.

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Section: Methodsmentioning

confidence: 99%

Changes in Spores of Bacillus megaterium Treated with Thioglycolate at a Low p H and Restoration of Germinability and Heat Resistance by Cations

Rowley

Levinson

1967

J Bacteriol

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“…The novel ingredients of this medium are manganous sulphate, magnesium sulphate and sodium pyruvate. Mn2f has been reported to be essential for sporulation of bacilli (Charney, Fisher & Hegarty, 1951) as has Mg2+ (Tallentire & Chiori, 1963). Mg2+ has also been implicated in determining the heat resistance of spores of B. naegateriurn (Tallentire & Chiori, 1963), and is present in Ellner's medium (1956).…”

Section: Sporulation Of Clostridia In Prepared Media and In Refortijementioning

confidence: 99%

“…Mn2f has been reported to be essential for sporulation of bacilli (Charney, Fisher & Hegarty, 1951) as has Mg2+ (Tallentire & Chiori, 1963). Mg2+ has also been implicated in determining the heat resistance of spores of B. naegateriurn (Tallentire & Chiori, 1963), and is present in Ellner's medium (1956). The peptone used by d'Aubert was replaced by Trypticase (BBL) at the same concentration, which in our experience supports better growth of most clostridia than do commercially available dehydrated peptones.…”

Section: Sporulation Of Clostridia In Prepared Media and In Refortijementioning

confidence: 99%