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A new sugarcane (Saccharum officinarum L.) fructanase which hydrolyzes both high molecular weight polysaccharides IFructose4:Galactitol5l,, (SP) and moderate-sized carbohydrates IFructose2:Galactitol33l,, (MMWC) has been purified from sugarcane juice. The Km, value has been estimated to be 33.7 micrograms per milliliter and 20 micrograms per milliliter for SP and MMWC, respectively. The optimal pH and temperature values are 6.0 and 300C, respectively. Purified protein has a pi value of 6.35 and a molecular weight of 13.2 kilodaltons. Fructanase activity appears to be Mn2 -dependent.

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Accurate and very sensitive HPLC methods which allow the simuJtaneous determination of the diamines putrescine (PUT) and cadaverine (CAD), resulting from ornithine decarboxylase (ODC) and lysine decarboxylase (LDC) activities or naturally occurring in sugarcane juices, are presented. Diamines were detected as their dansyl derivatives by fluorescence emission. The corresponding limits of detection were 21 ng mL 1 for PUTand 5 ng mL 1 for CAD. HPLC methods were compared with routine spectrophotometric assays.

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