Purification and Partial Characterization of a Fructanase which Hydrolyzes Natural Polysaccharides from Sugarcane Juice

Abstract

A new sugarcane (Saccharum officinarum L.) fructanase which hydrolyzes both high molecular weight polysaccharides IFructose4:Galactitol5l,, (SP) and moderate-sized carbohydrates IFructose2:Galactitol33l,, (MMWC) has been purified from sugarcane juice. The Km, value has been estimated to be 33.7 micrograms per milliliter and 20 micrograms per milliliter for SP and MMWC, respectively. The optimal pH and temperature values are 6.0 and 300C, respectively. Purified protein has a pi value of 6.35 and a molecular weight of 13.2 kilodaltons. Fructanase activity appears to be Mn2 -dependent.

Glycoproteins from sugarcane plants regulate cell polarity of Ustilago scitaminea teliospores

“…This could imply changes in the quantitative composition of glycoproteins. In fact, the size of the polysaccharide moiety of MMMG is lower than that of HMMG, as was found by Legaz et al (1990). These differences could affect the binding behaviour of sugarcane glycoproteins, which could be partially depending on the size of the molecule, although studies of the topography of the ligand distribution on the teliospore cell wall and of the size and polymerization status of the sugarcane glycoproteins are required to confirm this hypothesis.…”

“…Evidence of a selective binding of some of these glycoproteins, obtained from smut-sensitive Jaronu cv., to teliospores of U. scitaminea has recently been provided (Fontaniella et al, 2002). Homotypic adhesion in teliospores is produced by HMMG and MMMG, whose glycosidic moiety mainly contains b-1,2 fructan domains separated by galactitol units in ether linkage (Legaz et al, 1990). In a similar way, HMMG and MMMG obtained from smut-resistant Mayari cv (Fig.…”

Fructokinase, Fructans, Intestinal Permeability, and Metabolic Syndrome: An Equine Connection?

“…However, mammals do not produce fructanases, and hence fructans are considered indigestible polysaccharides. In contrast, fructanases are produced by some plants, fungi, yeast and bacteria [32, 34]. The primary bacteria producing fructanases are in the Firmicute phyla, and consist primarily of gram positive bacteria including Streptococcus salivarus , Strep mutans , Bacillus sp , Clostridia sp , Bifidobacterium , and others [32].…”

Bacteriological Study for Detection The Role of Fructan Produced from Pseudomonas putida as Enhancer for Lactobacillus acidophilus Growth

“…Microbial fructan is synthesized by recurring transfer of fructose from a fructosyl donor to the growing fructan chain, with much higher degree of polymerization (up to 100,000) than plant fructan (up to 150) [5]. Fructan producing bacteria were reported from the following genera: Pseudomonas, Zymomonas, Xanthomonas, Azotobacter, Erwinia, Streptococcus, Bacillus, and Arthobacter [6,7]. Fructan can be hydrolyzed by fructanases (inulinase and levanase) to generate fructose [8], mammals do not produce fructanases, and hence fructan is considered indigestible polysaccharides [6,9], therefore serve as substrate for utilization by the intestinal microflora and undergo fermentation in the colon by the colonic microflora resulting in the formation of acetate, lactate, and short chain of fatty acid which benefit the host health by simplifying the absorption of minerals and improving the host health [10].…”