Metabolic Responses of Sugarcane Plants Upon Different Plant–Pathogen Interactions

Legaz, Marı́a-Estrella; Sánchez-Elordi, Elena; Santiago, Rocío; Armas, R. de; Fontaniella, Blanca; Millanes, Ana M.; Bałanda, Maria; Vicente, Carlos

doi:10.1016/b978-0-12-812689-9.00013-3

Cited by 7 publications

(9 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in the lignification of smut-resistant plants has been detected by measuring cinnamyl alcohol dehydrogenase levels and by the overexpression of genes in RNAseq experiments of resistant varieties in the early moments of interaction [ 25 , 81 ]. The increase in lignin content after whip emission is probably not related to a protective host response; instead, it is likely a stage in the formation of the whip, which is composed of lignified plant tissue [ 82 ]. Recently, a proteomic approach developed after whip emission revealed 53 proteins related to lignin accumulation and oxidative stress at this stage of disease symptoms [ 83 ].…”

Section: Resultsmentioning

confidence: 99%

RNAseq Transcriptional Profiling following Whip Development in Sugarcane Smut Disease

et al. 2016

View full text Add to dashboard Cite

Sugarcane smut disease is caused by the biotrophic fungus Sporisorium scitamineum. The disease is characterized by the development of a whip-like structure from the primary meristems, where billions of teliospores are produced. Sugarcane smut also causes tillering and low sucrose and high fiber contents, reducing cane productivity. We investigated the biological events contributing to disease symptoms in a smut intermediate-resistant sugarcane genotype by examining the transcriptional profiles (RNAseq) shortly after inoculating the plants and immediately after whip emission. The overall picture of disease progression suggests that premature transcriptional reprogramming of the shoot meristem functions continues until the emergence of the whip. The guidance of this altered pattern is potentially primarily related to auxin mobilization in addition to the involvement of other hormonal imbalances. The consequences associated with whip emission are the modulation of typical meristematic functions toward reproductive organ differentiation, requiring strong changes in carbon partitioning and energy production. These changes include the overexpression of genes coding for invertases and trehalose-6P synthase, as well as other enzymes from key metabolic pathways, such as from lignin biosynthesis. This is the first report describing changes in the transcriptional profiles following whip development, providing a hypothetical model and candidate genes to further study sugarcane smut disease progression.

show abstract

Section: Resultsmentioning

confidence: 99%

RNAseq Transcriptional Profiling following Whip Development in Sugarcane Smut Disease

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Similar results on cytoagglutination were obtained using Herbaspirillum rubrisubalbicans treated with sugarcane glycoproteins of mid-(MMMG) and high molecular mass (HMMG). MMMG were preferentially desorbed from the bacterial cell wall with sucrose and galactitol, whereas HMMG were mainly desorbed with glucose and mannose [30]. This would indicate that, against this bacterium, MMMG behaves as signal molecules that bind to their receptor, or receptors, using their polysaccharide moiety, whereas, on the contrary, HMMG would use their peptide moiety for binding to different receptors, similar to the action mode of the lectin ConA [24], from Canavalia ensiformis.…”

Section: Receptorsmentioning

confidence: 96%

“…At this pole, filaments must be anchored to cell membrane, since invaginations are observed (2). Finally, depolymerization of F-actin is achieved at the opposite pole, the repolymerization of which leads to cell advancement (3) [30,52].…”

Section: Cytoskeleton Reorganization In S Scitamineum Cells In Respomentioning

confidence: 99%

“…GTP causes an enhancement of the inhibition exerted by blebbistatin during migration. However, a large reversion is achieved by addition of GTPγS, the poorly hydrolysable GTP analogue [30,55] or GDPβS, a deactivator of Rho [56] GTPases, and a total reversion of inhibition can be observed by combining GTP and GTPγS. GTP slightly reverts latrunculin A effect probably at the Rho pathway.…”

Section: Cytoskeleton Reorganization In S Scitamineum Cells In Respomentioning

confidence: 99%

See 1 more Smart Citation

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Sánchez-Elordi¹,

Díaz²,

Vicente³

et al. 2020

Parasitology and Microbiology Research

Self Cite

View full text Add to dashboard Cite

This study demonstrates the involvement of the cytoskeleton in the movement of cyanobacteria and fungal spores to their hosts to establish a state of symbiosis or pathogenicity. The term symbiosis sensu lato is referred not only to commensalism and mutualism but also to the parasitic aberrations. The establishment of association implies that the endohabitant can move on a wet surface until finding an entry point in the exohabitant surface. In aqueous media, the exohabitant secretes glycoproteins that form a chemoattraction gradient for the invading cells. In lichens, the gradient consists of fungal lectins whose function is to recognize a compatible green alga or cyanobacterium. In the case of pathogens, the secreted proteins usually are a mixture that includes false quorum and chemoattractant signals, and cell wall digestive enzymes. The results indicate that fungal lectins and defense proteins bind to specific cell wall receptors for signaling the activation of cytoskeleton, causing successive cycles of cell contraction-relaxation that permits the migration of the endohabitant. In this study, different biochemical and microscopy techniques have been used. The mechanisms through which the cytoskeleton carries out these cycles of cell contractionrelaxation are described, being this a remarkable advance compared to previous results.

show abstract

“…Xanthomonas albilineans is the only xanthomonad that produces albicidin, a phytotoxin responsible for a white leaf stripe with necrotic zones at leaf margin, rapid wilting and plant death. Albicidin is a DNA gyrase inhibitor that blocks chloroplast DNA replication and differentiation (Legaz et al, 2018). The disease can appear in three distinct forms: chronic, acute and latent.…”

Section: Introductionmentioning

confidence: 99%

Research Article Early down-regulation of chorismate mutase 2 gene expression in a sugarcane cultivar tolerant to <i>Xanthomonas</i> <i>albilineans</i> infection

Rosa-Santos¹,

Nogueira²,

Paz³

et al. 2019

Genet. Mol. Res.

View full text Add to dashboard Cite

Xanthomonas albilineans causes leaf scald disease, one of the most economically important diseases of sugarcane. Leaf scald is a systemic and vascular disease that can severely reduce productivity. The best and primary method of leaf scald control is the use of tolerant cultivars. Knowing that cell wall lignification is a plant defense mechanism and that chorismate mutase is relevant in this process, we analyzed the expression pattern of the chorismate mutase 2 (SoCM2) gene in two contrasting sugarcane cultivars by RT-qPCR. A similar pattern of downregulation for the SoCM2 gene was observed in both cultivars; however, after 72h, the tolerant cultivar (RB867515) showed repression of the SoCM2 gene, while for the susceptible cultivar (SP78-4467), SoCM2 gene downregulation occurred only after 144h. Here we propose that early T.M. repression of the SoCM2 gene is responsible for tolerance to X. albilineans infection in the sugarcane cultivar RB867515.

show abstract

Metabolic Responses of Sugarcane Plants Upon Different Plant–Pathogen Interactions

Cited by 7 publications

References 124 publications

RNAseq Transcriptional Profiling following Whip Development in Sugarcane Smut Disease

RNAseq Transcriptional Profiling following Whip Development in Sugarcane Smut Disease

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Research Article Early down-regulation of chorismate mutase 2 gene expression in a sugarcane cultivar tolerant to <i>Xanthomonas</i> <i>albilineans</i> infection

Contact Info

Product

Resources

About