Determination by high performance liquid chromatography of ornithine and lysine decaboxylases in sugar cane juices

Abstract

Accurate and very sensitive HPLC methods which allow the simuJtaneous determination of the diamines putrescine (PUT) and cadaverine (CAD), resulting from ornithine decarboxylase (ODC) and lysine decarboxylase (LDC) activities or naturally occurring in sugarcane juices, are presented. Diamines were detected as their dansyl derivatives by fluorescence emission. The corresponding limits of detection were 21 ng mL 1 for PUTand 5 ng mL 1 for CAD. HPLC methods were compared with routine spectrophotometric assays.

Effect of leaf scald (Xanthomonas albilineans) on polyamine and phenolic acid metabolism of two sugarcane cultivars

“…In previous studies, it has been found that several enzymes related to polyamine biosynthesis occurred in sugarcane juice (Legaz et al 2001). Arginase was shown to be a glycoprotein synthesized by sugarcane plants in response to U. scitaminea infection (Millanes et al 2005).…”

“…Assay of lysine decarboxylase LDC activity was assayed spectrophotometrically according to the method of Phan et al (1982) with the modifications described by Legaz et al (2001). The method is based upon the principle that CAD, a product of LDC activity, reacts with TNBS to give a product soluble in toluene.…”

“…Assay of ornithine decarboxylase ODC activity was assayed spectrophotometrically according to the method of Ngo et al (1987) with the modifications described by Legaz et al (2001). The method is based on the reaction of PUT, the product of ODC, with 2,4,6-trinitrobenzenesulfonic acid (TNBS) to give a coloured product, trinitrophenylputrescine (TNP-PUT) that was recovered in an organic, 1-pentanol phase.…”

Sugarcane glycoproteins bind to surface, specific ligands and modify cytoskeleton arrangement ofUstilago scitamineateliospores

“…Since the most active fraction of sugarcane glycoproteins is related to polyamine biosynthesis, we assesses arginase activity of sugarcane juice contains several enzymes related to polyamine biosynthesis, mainly ornithine decarboxylase (Legaz et al 2001), using ornithine as a substrate produced by enzymatic hydrolysis of arginine. Moreover, glycosilated arginase produced by lichenized and pathogenic ascomycetes binds to the algal partner and alters the level of putrescine produced by the host cell (Molina et al 1998).…”

Glycoproteins from sugarcane plants regulate cell polarity of Ustilago scitaminea teliospores

“…Arginase activity was measured since sugarcane juice contains several enzymes related to polyamine biosynthesis, mainly ornithine decarboxylase (Legaz et al, 2001), which uses ornithine produced by enzymatic hydrolysis of arginine as a substrate. Moreover, glycosilated arginase produced by lichenized and pathogenic ascomycetes binds to the algal partner or changes the level of putrescine produced by the invaded cell (Molina et al, 1998).…”