Mosquito is one of insects spreading the vector of serious diseases to human being through its bite such as Culex which spreads the vector of filariasis and brain inflammatory diseases. Some people have used plants as insecticides traditionally, such as fern (Diplazium esculentum (Retz.) Sw.). D. esculentum (Retz.) Sw. popularly known as edible fern which almost all Asian people, especially Indonesian consumed it as vegetable, and some people also used it for medicinal purposes. This study was performed to investigate the secondary metabolites from D. esculentum leaves and its larvicidal activity against Culex. The phytochemical screening was undertaken on the leaves and methanol extract of D. esculentum, the results showed the presence of alkaloids, steroids, phenols, and tannins on leaves, while methanol extract exhibited existence of steroids, saponins, phenols and tannins. Investigation of larvicidal activity of methanol extract was carried out against Culex larvaes for various concentrations which observed for 48 hours. Percentage of mortality was analyzed statistically using Bill Test method that showed active larvicidal activity with a LC50 value of 149.279 ppm. This study indicates that fern plant have the potential as larvicidal against Culex and need to perform the further research to develop the new natural insecticides.
Temurui ( Murraya koenigii [Linn.] Spreng) is a local plant of Aceh. The leaves of M. koenigii are used in most of the local foods as spices. Nowadays, cancer is claimed as the second deadly disease in the world where the number of sufferers increases every year. Cervical cancer (HeLa) is one of the most dominant cancers that happen in developing country, including Indonesia. Some chemotherapeutic agents using synthetic drugs have been used to treat cancer, but they are relatively expensive and cause poisoning that limits their use. Based on literatures, M. koenigii serves the potential secondary metabolites that could be developed as anticancer agent. This study aims to evaluate the cytotoxic activity from three extracts of M. koenigii leaves against HeLa cell line, including hexane, ethyl acetate, and methanol. Methodology used included extraction process, phytochemical screening, and cytotoxicity evaluation. The result showed that all the three extracts demonstrated a potent cytotoxic activity for HeLa cancer cells. Hexane and ethyl acetate showed a very strong cytotoxic effect with CD 50 values <1 μg/mL, whereas methanol extract showed cytotoxic effect with CD 50 value of 2.25 μg/mL. These results prove the potential of M. koenigii as an anticancer agent.
Beta Karoten merupakan provitamin A yang dapat diubah didalam tubuh menjadi vitamin A setelah mengalami proses metabolisme. Beta karoten merupakan salah satu parameter yang menentukan kualitas CPO dalam perdagangan internasional. Penelitian ini bertujuan untuk mengetahui kandungan beta karoten pada sampel CPO (Crude Palm Oil) menggunakan metode spektrofotometri UV-Vis. Metode yang digunakan dalam analisis beta karoten adalah metode standar MPOB (Malaysian Palm Oil Board). Hasil analisis menggunakan spektrofotometer UV-Vis menunjukkan bahwa kandungan beta karoten dari masing-masing sampel berturut-turut adalah sebagai berikut: Sampel A(330,00 ppm), Sampel B (346,26 ppm), Sampel C(358,70 ppm), Sampel D(549,05 ppm), dan Sampel E (533,75 ppm). Berdasarkan hasil analisis UV Vis terhadap sampel dapat disimpulkan bahwa sampel D memiliki kandungan beta karoten yang paling tinggi dibandingkan dengan sampel CPO lainnya.
Waste cooking oil contains carcinogenic compounds which are formed during the frying process. The content change makes it is not worth for reuse cooking oil. One of the damages to cooking oil is caused by heating. The quality of useable oil refers to SNI 01-3741-2013. The sample used in this research is waste cooking oil that is used for 5 times of frying. The quality of the oil can be improved using Nano-Montmorillonite adsorbent. The results of PSA and FTIR characterization show that Nano-Montmorillonite has a nano-scale particle size of 15.3 nm with a percentage of 6%. The rest are particles that have a size> 100 nm with a total percentage of 94% with a respective size of 383.9 nm with a percentage of 59% and 6647.1 nm with a percentage of 35% and have functional groups, namely hydroxyl (-OH), Si-O-Si and Al-Al-OH. Testing of waste cooking oil quality includes organoleptic, acid numbers, peroxide numbers, and saponification numbers using Nano-Montmorillonite. Based on the results, the more Nano-Montmorillonite adsorbent was added, the lower the numbers of acid, peroxide, and saponification in waste cooking oil and the better the quality of flavor and color. Based on this analysis, Nano-Montmorillonite adsorbent can improve the quality of waste cooking oil.Abstrak: Minyak jelantah mengandung senyawa yang bersifat karsinogenik yang terbentuk selama proses penggorengan. Perubahan sifat ini menjadikan minyak goreng tersebut tidak layak digunakan kembali sebagai bahan makanan. Salah satu kerusakan pada minyak goreng disebabkan oleh pemanasan. Kualitas minyak layak pakai merujuk pada SNI 01-3741-2013. Sampel minyak yang digunakan dalam penelitian ini adalah minyak jelantah dengan 5 kali penggorengan. Kualitas minyak tersebut dapat ditingkatkan menggunakan adsorben Nano-Montmorillonit. Berdasarkan hasil karakterisasi PSA dan FTIR menunjukkan bahwa Nano-Montmorillonit memiliki ukuran partikel berskala nano yaitu 15,3 nm dengan persentase sebesar 6% dan sisanya merupakan partikel yang memiliki ukuran > 100 nm dengan total persentase 94% dengan ukuran masing-masing sebesar 383,9 nm dengan persentase 59% dan 6647,1 nm dengan persentase 35%serta memiliki gugus-gugus fungsional yaitu hidroksil (-OH), Si-O-Si dan Al-Al-OH. Pengujian kualitas minyak jelantah meliputi organoleptik, bilangan asam, bilangan peroksida dan bilangan penyabunan menggunakan Nano-Montmorillonit. Berdasarkan hasil pengujian maka semakin banyak jumlah adsorben Nano-Montmorilonit yang ditambahkan maka bilangan asam, bilangan peroksida, bilangan penyabunan mengalami penurunan serta kualitas aroma dan warna pada minyak jelantah menjadi lebih baik. Berdasarkan analisis tersebut adsorben Nano-Montmorillonit dapat meningkatkan kualitas minyak jelantah.
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