species are the obligate tissue protozoan parasites of livestock causing clinical and subclinical disease resulting in downgrading of the meat and their products thereby leading to economic losses. The present study reveals the prevalence and distribution of sarcocystosis in water buffaloes () slaughtered at local abattoirs in A.P for a period of 1 year from June 2014 to May 2015. A total of 137 buffalo carcasses were screened grossly and microscopically organ wise viz., esophagus, tongue, heart, diaphragm and intercostal muscles. Out of 137 screened, 91 were infected with an overall prevalence of 66.42 %. Age wise analysis of 89 young male calves aged about 18-36 months old revealed 65.16 % (58/89) and 48 old she buffaloes (5-8 years) showed 68.75 % (33/48). The organ wise prevalence was highest in esophagus (51.82 %) followed by tongue (47.44 %), heart (29.92 %), diaphragmatic muscles (28.46 %) and intercostal muscles (18.24 %), respectively. Morphometric studies revealed the presence of two species, i.e., and infection with a prevalence of 43.79 and 22.62 %, respectively, along with mixed infection rate of 43.06 %. Microscopic studies of showed sarcocyst length/width/cyst wall thickness ranged between 0.31-0.69/0.09-0.12 mm/<1 µm, respectively, and bradyzoites with an average of 6.25 µm length/2.5 µm width. Similarly, cyst ranged between 2 and 8.5 mm/1-3 mm/2-5 µm and bradyzoites with an average of 10 µm length/2.5 µm width. The histopathological studies revealed congestion and degenerative changes of myocytes along with infiltration of mononuclear cells.
The field isolates of Trypanosoma evansi was collected from the infected cattle and it was propagated in rats. Trypanosoma evansi parasites were separated from the blood of infected rats by using diethylaminoethyl cellulose column chromatography. Whole cell lysate antigen (WCL) was prepared from purified trypanosomes by ultrasonication and centrifugation. The prepared WCL antigen was further purified by 50 % ammonium sulphate precipitation. Protein concentration of WCL antigen of T. evansi was 60 mg/ml. Protein concentration was adjusted to 1.0 mg/ml in PBS, pH 8.0 and stored at -20 0 C. Polypeptide profiles of WCL antigen of T. evansi was determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. A total of eight polypeptide bands of the size ranging from 25 to 85 kDa in WCL antigen of T. evansi were obtained. Five prominent bands with molecular weight of 74, 60, 53, 42 and 37 kDa and three light bands with molecular weight of 85, 34 and 25 kDa were observed.
The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in bovines. The optimum concentration of antigen, test sera and conjugate were determined as 5 lg per well, 1:10 and 1: 6,000 dilutions, respectively. Among 320 cattle and 382 buffaloes examined in different parts of Rayalaseema region of Andhra Pradesh for T. evansi infection, 36.12 and 31.87 percent were found positive by indirect ELISA, respectively.
Amphistomiasis, caused by species of Paramphistomatidae is an economically important disease in ruminants. is a very common amphistome in bile ducts and gall bladder of cattle and buffaloes worldwide where as in goats, it is exclusively found in Asian countries. Screening of livers from 100 sheep and 154 goats during slaughter at local slaughter houses from October, 2014 to April, 2015 in Andhra Pradesh (India) revealed presence of amphistomes in main bile ducts and gall bladder in three goats (1.9%) and were not observed in livers of sheep. Grossly, the affected livers were congested and the bile ducts were firm, thickened and occluded with amphistomes along with light yellowish foul smelling fluid. There were no apparent changes in gall bladder. Amphistomes were identified as by the standard staining technique using borax caramine. Microscopically, sections of liver revealed areas of haemarrhage, necrosis and infiltration of mononuclear cells in the parenchyma and surrounding the bile ducts. The wall of bile ducts revealed connective tissue proliferation with a characteristic mucosal plug of bile duct drawn into the acetabulum. There was hyperplasia of bile duct epithelium along with marked proliferation of mucosal glands and mononuclear cell infiltration. This paper appears to be the first report of in goats from Andhra Pradesh, India.
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