Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

Sivajothi, S.; Rayulu, V. C.; Malakondaiah, P.; Sreenivasulu, D.

doi:10.1007/s12639-014-0465-z

Cited by 9 publications

(7 citation statements)

References 9 publications

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“…Different diagnostic methods were developed to diagnose T. evansi in the animals at the field level also (Sivajothi et al 2012;2013b;2014c). The variation of the antigen can cause development of variable severity of the infection and changes in the development of the symptoms.…”

Section: Resultsmentioning

confidence: 99%

“…Protein concentration of the WCL Ag of T. evansi prepared in the present study was estimated (60 mg/ml). Protein concentration was adjusted to 1.0 mg/ml in PBS, pH 8.0 and stored at -20°C in 1.0 ml aliquots (Sivajothi et al 2014c). Polypeptide profile of WCL of T. evansi was determined by SDS-PAGE as described by Laemmli (1970).…”

Section: Preparation Of Wcl Ag Of T Evansimentioning

confidence: 99%

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Polypeptide profiles of South Indian isolate of Trypanosoma evansi

Sivajothi¹,

Rayulu²,

Reddy

et al. 2014

J Parasit Dis

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The field isolates of Trypanosoma evansi was collected from the infected cattle and it was propagated in rats. Trypanosoma evansi parasites were separated from the blood of infected rats by using diethylaminoethyl cellulose column chromatography. Whole cell lysate antigen (WCL) was prepared from purified trypanosomes by ultrasonication and centrifugation. The prepared WCL antigen was further purified by 50 % ammonium sulphate precipitation. Protein concentration of WCL antigen of T. evansi was 60 mg/ml. Protein concentration was adjusted to 1.0 mg/ml in PBS, pH 8.0 and stored at -20 0 C. Polypeptide profiles of WCL antigen of T. evansi was determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. A total of eight polypeptide bands of the size ranging from 25 to 85 kDa in WCL antigen of T. evansi were obtained. Five prominent bands with molecular weight of 74, 60, 53, 42 and 37 kDa and three light bands with molecular weight of 85, 34 and 25 kDa were observed.

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Wcl Ag Of T Evansimentioning

confidence: 99%

Polypeptide profiles of South Indian isolate of Trypanosoma evansi

Sivajothi¹,

Rayulu²,

Reddy

et al. 2014

J Parasit Dis

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“…Clinical signs may vary based on the severity of the infection. Prevalence of T.evansi was recorded in different species of animals by different diagnostic methods in India [2,3]. Haematological and biochemical abnormalities were also recorded in experimental animal infection with T.evansi [4].…”

Section: Introductionmentioning

confidence: 99%

Haematological changes in Trypanosoma evansi infected cattle

Sivajothi

Reddy²,

Kumari³

et al. 2014

International Journal of Scientific World

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During the three years period of study twelve cross bred cattle were identified that they were suffering with clinical Trypanosomiasis. Up on clinical examination cattle had anorexia, fever, ocular discharges, dullness and enlarged lymph nodes. Stained peripheral blood examination revealed the presence of Trypanosoma evansi. Haematological abnormalities include decreased TEC, PCV, Hb and TLC values (P<0.01). The present leucopaenia was characterized by neutrophilia, eosinophilia and lymphocytopaenia (P>0.05).

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“…; Sivajothi et al . ). In our previous work, ELISA, IFM, gold‐based immunochromatographic strip assay and lanthanide‐labelled immunochromatographic strip testing, all based on polyclonal antibodies, were proposed and successfully used for the detection of ancient proteinous fibres and fabrics (Liu et al .…”

Section: Introductionmentioning

confidence: 97%

Development of an enzyme‐linked immunosorbent assay based on the monoclonal antibody of Bombyx mori to detect ancient silk

Zheng

Dai

et al. 2019

Archaeometry

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Identifying the traces and residues of unearthed silk fibres is crucial for further analysis and study. In this study, an enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies specific to Bombyx mori fibroin was developed in order to for detect ancient silk. Five monoclonal antibodies were successfully prepared based on fibroin hydrolysate, and four had a high specificity for mulberry silk. Furthermore, they could be used to discern B. mori fibre from tussah fibre and eri fibre. The ELISA standard curve of the most sensitive monoclonal antibody, anti-Silk Fibroin (SF)-3, was y = 0.022x + 0.177 (R 2 = 0.998), and the detection limit was 4.65 ng/ml. Using this antibody, we successfully identified five ancient mulberry silk samples from different time periods. The results suggest that ELISA assays based on monoclonal antibodies can be used as an immunological testing method for unearthed silk fibre.

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Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

Cited by 9 publications

References 9 publications

Polypeptide profiles of South Indian isolate of Trypanosoma evansi

Polypeptide profiles of South Indian isolate of Trypanosoma evansi

Haematological changes in Trypanosoma evansi infected cattle

Development of an enzyme‐linked immunosorbent assay based on the monoclonal antibody of Bombyx mori to detect ancient silk

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