Elective surgery was used as a model of severe non-thyroidal illness (SNTI) to study the inter-relation between changes in serum thyroid hormones, thyroid stimulating hormone (TSH), cortisol, and interleukin 6 concentrations. The study was designed to determine whether the expected interleukin 6 increases after surgery are the cause of decreased serum tri-iodothyronine (T,) Total T, free T, free T4, and TSH concentrations were measured using an "Amerlite" (Kodal Clinical Diagnostics, Amersham, UK) automated enzyme immunoassay (luminescence signal), the lower limit of detection (LLD) for TSH being 0 03 mU/l. Serum interleukin 6 concentrations were measured using an ELISA ("Biotrak"; Kodak) with a LLD of 1 pg/ml and coefficients of variation of 3-5% (intra-assay) and 7 0% (interassay). Cortisol concentrations were measured using a ("Amerlex"; Kodak) radioimmunoassay.Results
Nineteen patient blood samples each with modified hematocrit concentrations of approximately 20, 30, 40, 50, and 60%, were assayed for their glucose concentration by the Glucometer II. Blood removal from the test strip was by the one- and two-blot techniques. The reference method was the Yellow Springs Instruments (YSI) blood glucose analyzer. Glucometer II results were falsely high for the single blot (13-59%, mean 33%) and double blot (12-41%, mean 19%) at 20% hematocrit and falsely low at 60% hematocrit for the single blot (22-44%, mean 37%) and the double blot (26-49%, mean 43%). At 40-50% hematocrit, Glucometer II and YSI results agreed only for the one-blot technique.
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